Strain Fitness in Escherichia coli BW25113 around b1268

Experiment: LB + carbenicillin Time5hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciV and yciO overlap by 4 nucleotidesyciO and yciQ are separated by 27 nucleotidesyciQ and rluB are separated by 210 nucleotides b1266: yciV - hypothetical protein (NCBI), at 1,321,244 to 1,322,125 yciV b1267: yciO - orf, hypothetical protein (VIMSS), at 1,322,122 to 1,322,742 yciO b1268: yciQ - predicted inner membrane protein (NCBI), at 1,322,770 to 1,324,665 yciQ b1269: rluB - 23S rRNA pseudouridylate synthase (NCBI), at 1,324,876 to 1,325,751 rluB Position (kb) 1322 1323 1324 1325Strain fitness (log2 ratio) -2 -1 0 1 2at 1321.935 kb on - strand, within yciVat 1322.096 kb on + strandat 1322.096 kb on + strandat 1322.161 kb on - strandat 1322.231 kb on + strand, within yciOat 1322.708 kb on + strandat 1322.713 kb on + strandat 1322.766 kb on - strandat 1322.814 kb on + strandat 1322.880 kb on + strandat 1322.880 kb on + strandat 1323.017 kb on + strand, within yciQat 1323.232 kb on + strand, within yciQat 1323.240 kb on - strand, within yciQat 1323.364 kb on + strand, within yciQat 1323.420 kb on + strand, within yciQat 1323.420 kb on + strand, within yciQat 1323.421 kb on + strand, within yciQat 1323.421 kb on + strand, within yciQat 1323.428 kb on - strand, within yciQat 1323.428 kb on - strand, within yciQat 1323.491 kb on + strand, within yciQat 1323.543 kb on + strand, within yciQat 1323.571 kb on - strand, within yciQat 1323.650 kb on - strand, within yciQat 1323.742 kb on + strand, within yciQat 1323.755 kb on - strand, within yciQat 1323.792 kb on - strand, within yciQat 1323.819 kb on + strand, within yciQat 1323.827 kb on - strand, within yciQat 1323.827 kb on - strand, within yciQat 1323.849 kb on + strand, within yciQat 1323.849 kb on + strand, within yciQat 1323.855 kb on - strand, within yciQat 1323.881 kb on - strand, within yciQat 1323.883 kb on + strand, within yciQat 1323.883 kb on + strand, within yciQat 1323.891 kb on - strand, within yciQat 1323.891 kb on - strand, within yciQat 1323.891 kb on - strand, within yciQat 1323.891 kb on - strand, within yciQat 1323.891 kb on - strand, within yciQat 1324.018 kb on + strand, within yciQat 1324.018 kb on + strand, within yciQat 1324.019 kb on - strand, within yciQat 1324.026 kb on - strand, within yciQat 1324.064 kb on - strand, within yciQat 1324.131 kb on + strand, within yciQat 1324.139 kb on - strand, within yciQat 1324.139 kb on - strand, within yciQat 1324.235 kb on + strand, within yciQat 1324.236 kb on + strand, within yciQat 1324.236 kb on + strand, within yciQat 1324.243 kb on - strand, within yciQat 1324.389 kb on + strand, within yciQat 1324.389 kb on + strand, within yciQat 1324.398 kb on - strand, within yciQat 1324.398 kb on - strand, within yciQat 1324.491 kb on + strandat 1324.821 kb on + strandat 1324.826 kb on + strandat 1324.829 kb on - strandat 1324.829 kb on - strandat 1325.097 kb on + strand, within rluBat 1325.110 kb on + strand, within rluBat 1325.179 kb on + strand, within rluBat 1325.179 kb on + strand, within rluBat 1325.635 kb on - strand, within rluBat 1325.636 kb on - strand, within rluBat 1325.636 kb on - strand, within rluB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time5hr
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1,321,935 - yciV b1266 0.78 -0.5
1,322,096 + -1.1
1,322,096 + -0.0
1,322,161 - -0.3
1,322,231 + yciO b1267 0.18 +0.8
1,322,708 + -0.4
1,322,713 + +0.0
1,322,766 - -0.7
1,322,814 + +0.4
1,322,880 + -0.8
1,322,880 + +0.7
1,323,017 + yciQ b1268 0.13 -0.6
1,323,232 + yciQ b1268 0.24 -1.8
1,323,240 - yciQ b1268 0.25 +0.9
1,323,364 + yciQ b1268 0.31 -0.6
1,323,420 + yciQ b1268 0.34 +1.5
1,323,420 + yciQ b1268 0.34 +0.2
1,323,421 + yciQ b1268 0.34 -2.3
1,323,421 + yciQ b1268 0.34 -1.5
1,323,428 - yciQ b1268 0.35 +0.3
1,323,428 - yciQ b1268 0.35 +0.9
1,323,491 + yciQ b1268 0.38 -1.4
1,323,543 + yciQ b1268 0.41 -0.4
1,323,571 - yciQ b1268 0.42 -0.4
1,323,650 - yciQ b1268 0.46 -0.1
1,323,742 + yciQ b1268 0.51 -0.4
1,323,755 - yciQ b1268 0.52 +0.9
1,323,792 - yciQ b1268 0.54 +1.9
1,323,819 + yciQ b1268 0.55 -1.1
1,323,827 - yciQ b1268 0.56 +0.9
1,323,827 - yciQ b1268 0.56 +0.9
1,323,849 + yciQ b1268 0.57 +0.8
1,323,849 + yciQ b1268 0.57 -0.2
1,323,855 - yciQ b1268 0.57 +0.0
1,323,881 - yciQ b1268 0.59 -0.8
1,323,883 + yciQ b1268 0.59 +0.1
1,323,883 + yciQ b1268 0.59 -0.8
1,323,891 - yciQ b1268 0.59 -0.1
1,323,891 - yciQ b1268 0.59 +0.3
1,323,891 - yciQ b1268 0.59 -0.1
1,323,891 - yciQ b1268 0.59 +2.5
1,323,891 - yciQ b1268 0.59 +0.9
1,324,018 + yciQ b1268 0.66 -0.5
1,324,018 + yciQ b1268 0.66 -1.1
1,324,019 - yciQ b1268 0.66 +0.9
1,324,026 - yciQ b1268 0.66 -0.3
1,324,064 - yciQ b1268 0.68 +0.9
1,324,131 + yciQ b1268 0.72 -0.1
1,324,139 - yciQ b1268 0.72 +0.9
1,324,139 - yciQ b1268 0.72 -0.4
1,324,235 + yciQ b1268 0.77 -0.1
1,324,236 + yciQ b1268 0.77 -0.6
1,324,236 + yciQ b1268 0.77 -0.4
1,324,243 - yciQ b1268 0.78 +0.9
1,324,389 + yciQ b1268 0.85 +0.1
1,324,389 + yciQ b1268 0.85 -0.8
1,324,398 - yciQ b1268 0.86 -1.2
1,324,398 - yciQ b1268 0.86 -1.4
1,324,491 + -0.1
1,324,821 + -0.2
1,324,826 + +0.2
1,324,829 - -1.9
1,324,829 - -0.1
1,325,097 + rluB b1269 0.25 -0.1
1,325,110 + rluB b1269 0.27 +0.1
1,325,179 + rluB b1269 0.35 -0.8
1,325,179 + rluB b1269 0.35 +0.6
1,325,635 - rluB b1269 0.87 +0.6
1,325,636 - rluB b1269 0.87 -0.9
1,325,636 - rluB b1269 0.87 -1.6

Or see this region's nucleotide sequence