Experiment: LB + carbenicillin Time5hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yciI and tonB are separated by 223 nucleotides tonB and yciA are separated by 39 nucleotides yciA and yciB are separated by 104 nucleotides yciB and yciC are separated by 29 nucleotides
b1251: yciI - orf, hypothetical protein (VIMSS), at 1,308,593 to 1,308,889
yciI
b1252: tonB - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 1,309,113 to 1,309,832
tonB
b1253: yciA - predicted hydrolase (NCBI), at 1,309,872 to 1,310,270
yciA
b1254: yciB - intracellular septation protein A (NCBI), at 1,310,375 to 1,310,914
yciB
b1255: yciC - hypothetical protein (NCBI), at 1,310,944 to 1,311,687
yciC
Position (kb)
1309
1310
1311 Strain fitness (log2 ratio)
-2
-1
0
1 at 1309.193 kb on + strand, within tonB at 1309.201 kb on - strand, within tonB at 1309.201 kb on - strand, within tonB at 1309.201 kb on - strand, within tonB at 1309.474 kb on + strand, within tonB at 1309.533 kb on - strand, within tonB at 1309.533 kb on - strand, within tonB at 1309.610 kb on + strand, within tonB at 1309.705 kb on - strand, within tonB at 1309.705 kb on - strand, within tonB at 1310.036 kb on + strand, within yciA at 1310.036 kb on + strand, within yciA at 1310.046 kb on + strand, within yciA at 1310.061 kb on + strand, within yciA at 1310.141 kb on - strand, within yciA at 1310.410 kb on + strand at 1310.416 kb on - strand at 1310.650 kb on - strand, within yciB at 1310.809 kb on - strand, within yciB at 1311.105 kb on + strand, within yciC at 1311.105 kb on + strand, within yciC at 1311.187 kb on - strand, within yciC at 1311.189 kb on + strand, within yciC at 1311.212 kb on - strand, within yciC at 1311.212 kb on - strand, within yciC
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time5hr remove 1,309,193 + tonB b1252 0.11 +0.1 1,309,201 - tonB b1252 0.12 +0.1 1,309,201 - tonB b1252 0.12 -1.7 1,309,201 - tonB b1252 0.12 +0.2 1,309,474 + tonB b1252 0.50 +0.5 1,309,533 - tonB b1252 0.58 +0.1 1,309,533 - tonB b1252 0.58 -0.7 1,309,610 + tonB b1252 0.69 +0.1 1,309,705 - tonB b1252 0.82 +0.2 1,309,705 - tonB b1252 0.82 -0.1 1,310,036 + yciA b1253 0.41 -0.7 1,310,036 + yciA b1253 0.41 -2.1 1,310,046 + yciA b1253 0.44 -0.5 1,310,061 + yciA b1253 0.47 -0.4 1,310,141 - yciA b1253 0.67 -0.5 1,310,410 + -0.1 1,310,416 - -0.2 1,310,650 - yciB b1254 0.51 +0.9 1,310,809 - yciB b1254 0.80 +0.4 1,311,105 + yciC b1255 0.22 -0.1 1,311,105 + yciC b1255 0.22 +0.4 1,311,187 - yciC b1255 0.33 +0.6 1,311,189 + yciC b1255 0.33 -0.3 1,311,212 - yciC b1255 0.36 +0.0 1,311,212 - yciC b1255 0.36 -0.4
Or see this region's nucleotide sequence