Experiment: LB + carbenicillin Time5hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafH and lpcA are separated by 239 nucleotides lpcA and yafJ are separated by 205 nucleotides yafJ and yafK overlap by 30 nucleotides
b0221: yafH - putative acyl-CoA dehydrogenase (EC 1.3.99.-) (VIMSS), at 240,859 to 243,303
yafH
b0222: lpcA - phosphoheptose isomerase (NCBI), at 243,543 to 244,121
lpcA
b0223: yafJ - predicted amidotransfease (NCBI), at 244,327 to 245,094
yafJ
b0224: yafK - hypothetical protein (NCBI), at 245,065 to 245,805
yafK
Position (kb) 243
244
245 Strain fitness (log2 ratio) -2
-1
0
1 at 242.599 kb on - strand, within yafH at 242.660 kb on + strand, within yafH at 242.660 kb on + strand, within yafH at 242.670 kb on - strand, within yafH at 242.822 kb on + strand, within yafH at 242.831 kb on - strand, within yafH at 242.831 kb on - strand, within yafH at 242.962 kb on + strand, within yafH at 243.011 kb on + strand, within yafH at 243.011 kb on + strand, within yafH at 243.019 kb on - strand, within yafH at 243.063 kb on + strand at 243.183 kb on - strand at 243.211 kb on - strand at 243.261 kb on + strand at 243.270 kb on + strand at 243.270 kb on + strand at 243.467 kb on + strand at 243.501 kb on - strand at 243.670 kb on - strand, within lpcA at 243.717 kb on + strand, within lpcA at 243.853 kb on + strand, within lpcA at 243.853 kb on + strand, within lpcA at 243.866 kb on - strand, within lpcA at 243.866 kb on - strand, within lpcA at 243.893 kb on - strand, within lpcA at 243.903 kb on + strand, within lpcA at 244.003 kb on + strand, within lpcA at 244.070 kb on - strand at 244.078 kb on + strand at 244.080 kb on - strand at 244.124 kb on - strand at 244.129 kb on - strand at 244.159 kb on + strand at 244.197 kb on + strand at 244.217 kb on - strand at 244.311 kb on + strand at 244.311 kb on + strand at 244.319 kb on - strand at 244.371 kb on - strand at 244.396 kb on - strand at 244.396 kb on - strand at 244.442 kb on + strand, within yafJ at 244.453 kb on + strand, within yafJ at 244.516 kb on + strand, within yafJ at 244.551 kb on + strand, within yafJ at 244.564 kb on - strand, within yafJ at 244.564 kb on - strand, within yafJ at 244.616 kb on + strand, within yafJ at 244.616 kb on + strand, within yafJ at 244.637 kb on - strand, within yafJ at 244.728 kb on + strand, within yafJ at 244.763 kb on - strand, within yafJ at 244.799 kb on - strand, within yafJ at 244.799 kb on - strand, within yafJ at 244.868 kb on + strand, within yafJ at 245.001 kb on - strand, within yafJ at 245.001 kb on - strand, within yafJ at 245.011 kb on - strand, within yafJ at 245.083 kb on + strand at 245.117 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time5hr remove 242,599 - yafH b0221 0.71 +0.5 242,660 + yafH b0221 0.74 -0.3 242,660 + yafH b0221 0.74 -0.2 242,670 - yafH b0221 0.74 -0.3 242,822 + yafH b0221 0.80 -0.0 242,831 - yafH b0221 0.81 -0.2 242,831 - yafH b0221 0.81 +0.1 242,962 + yafH b0221 0.86 +0.1 243,011 + yafH b0221 0.88 +0.4 243,011 + yafH b0221 0.88 +0.9 243,019 - yafH b0221 0.88 +0.1 243,063 + +0.8 243,183 - -0.0 243,211 - -0.8 243,261 + -0.1 243,270 + +0.4 243,270 + -0.2 243,467 + -0.2 243,501 - +0.1 243,670 - lpcA b0222 0.22 +0.3 243,717 + lpcA b0222 0.30 +0.7 243,853 + lpcA b0222 0.54 -0.3 243,853 + lpcA b0222 0.54 -0.7 243,866 - lpcA b0222 0.56 +0.3 243,866 - lpcA b0222 0.56 -2.4 243,893 - lpcA b0222 0.60 -0.9 243,903 + lpcA b0222 0.62 +0.7 244,003 + lpcA b0222 0.79 -0.6 244,070 - -0.3 244,078 + -1.0 244,080 - +0.2 244,124 - -0.0 244,129 - -0.1 244,159 + +0.7 244,197 + -0.0 244,217 - -0.1 244,311 + +0.1 244,311 + -0.0 244,319 - -0.2 244,371 - +0.1 244,396 - +0.6 244,396 - -0.1 244,442 + yafJ b0223 0.15 +0.1 244,453 + yafJ b0223 0.16 +0.1 244,516 + yafJ b0223 0.25 +0.0 244,551 + yafJ b0223 0.29 -0.0 244,564 - yafJ b0223 0.31 -0.4 244,564 - yafJ b0223 0.31 -0.3 244,616 + yafJ b0223 0.38 +0.0 244,616 + yafJ b0223 0.38 -0.9 244,637 - yafJ b0223 0.40 +0.2 244,728 + yafJ b0223 0.52 -0.4 244,763 - yafJ b0223 0.57 +0.2 244,799 - yafJ b0223 0.61 -0.2 244,799 - yafJ b0223 0.61 -0.3 244,868 + yafJ b0223 0.70 +0.5 245,001 - yafJ b0223 0.88 -0.1 245,001 - yafJ b0223 0.88 +0.1 245,011 - yafJ b0223 0.89 +0.1 245,083 + -0.0 245,117 - +0.3
Or see this region's nucleotide sequence