Experiment: LB + carbenicillin Time3hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydeH and ydeI are separated by 254 nucleotides ydeI and ydeJ are separated by 275 nucleotides ydeJ and dcp are separated by 43 nucleotides
b1535: ydeH - hypothetical protein (NCBI), at 1,620,984 to 1,621,874
ydeH
b1536: ydeI - hypothetical protein (NCBI), at 1,622,129 to 1,622,521
ydeI
b1537: ydeJ - competence damage-inducible protein A (NCBI), at 1,622,797 to 1,623,315
ydeJ
b1538: dcp - dipeptidyl carboxypeptidase II (NCBI), at 1,623,359 to 1,625,404
dcp
Position (kb)
1622
1623
1624 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1621.799 kb on + strand at 1621.799 kb on + strand at 1621.799 kb on + strand at 1621.803 kb on + strand at 1621.811 kb on - strand at 1621.813 kb on - strand at 1621.917 kb on - strand at 1621.961 kb on + strand at 1621.973 kb on + strand at 1622.143 kb on - strand at 1622.227 kb on + strand, within ydeI at 1622.299 kb on - strand, within ydeI at 1622.299 kb on - strand, within ydeI at 1622.405 kb on - strand, within ydeI at 1622.405 kb on - strand, within ydeI at 1622.409 kb on + strand, within ydeI at 1622.409 kb on + strand, within ydeI at 1622.420 kb on + strand, within ydeI at 1622.420 kb on + strand, within ydeI at 1622.420 kb on + strand, within ydeI at 1622.471 kb on - strand, within ydeI at 1622.527 kb on + strand at 1622.774 kb on - strand at 1622.834 kb on + strand at 1622.835 kb on - strand at 1622.838 kb on - strand at 1622.868 kb on + strand, within ydeJ at 1622.868 kb on + strand, within ydeJ at 1622.934 kb on + strand, within ydeJ at 1622.983 kb on + strand, within ydeJ at 1622.987 kb on + strand, within ydeJ at 1623.043 kb on + strand, within ydeJ at 1623.097 kb on + strand, within ydeJ at 1623.097 kb on + strand, within ydeJ at 1623.170 kb on + strand, within ydeJ at 1623.211 kb on + strand, within ydeJ at 1623.259 kb on + strand, within ydeJ at 1623.259 kb on + strand, within ydeJ at 1623.297 kb on + strand at 1623.297 kb on + strand at 1623.309 kb on - strand at 1623.309 kb on - strand at 1623.411 kb on - strand at 1623.449 kb on + strand at 1623.449 kb on + strand at 1623.501 kb on + strand at 1623.519 kb on - strand at 1623.739 kb on + strand, within dcp at 1623.739 kb on + strand, within dcp at 1623.752 kb on - strand, within dcp at 1623.772 kb on - strand, within dcp at 1623.772 kb on - strand, within dcp at 1623.801 kb on + strand, within dcp at 1623.844 kb on - strand, within dcp at 1623.892 kb on - strand, within dcp at 1623.951 kb on + strand, within dcp at 1624.060 kb on - strand, within dcp at 1624.089 kb on + strand, within dcp at 1624.237 kb on - strand, within dcp at 1624.297 kb on - strand, within dcp
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time3hr remove 1,621,799 + +2.0 1,621,799 + +2.0 1,621,799 + +2.0 1,621,803 + +1.0 1,621,811 - +1.0 1,621,813 - +1.0 1,621,917 - +1.0 1,621,961 + +1.0 1,621,973 + +0.5 1,622,143 - +0.4 1,622,227 + ydeI b1536 0.25 +1.0 1,622,299 - ydeI b1536 0.43 +0.0 1,622,299 - ydeI b1536 0.43 -0.3 1,622,405 - ydeI b1536 0.70 +0.3 1,622,405 - ydeI b1536 0.70 +1.0 1,622,409 + ydeI b1536 0.71 +1.0 1,622,409 + ydeI b1536 0.71 +1.0 1,622,420 + ydeI b1536 0.74 +1.0 1,622,420 + ydeI b1536 0.74 +1.0 1,622,420 + ydeI b1536 0.74 +0.0 1,622,471 - ydeI b1536 0.87 +1.0 1,622,527 + +1.0 1,622,774 - +0.2 1,622,834 + +1.1 1,622,835 - -1.3 1,622,838 - -0.3 1,622,868 + ydeJ b1537 0.14 +1.1 1,622,868 + ydeJ b1537 0.14 +1.1 1,622,934 + ydeJ b1537 0.26 +0.1 1,622,983 + ydeJ b1537 0.36 -0.1 1,622,987 + ydeJ b1537 0.37 -1.1 1,623,043 + ydeJ b1537 0.47 +0.1 1,623,097 + ydeJ b1537 0.58 -1.8 1,623,097 + ydeJ b1537 0.58 -1.1 1,623,170 + ydeJ b1537 0.72 -1.3 1,623,211 + ydeJ b1537 0.80 +0.6 1,623,259 + ydeJ b1537 0.89 -0.5 1,623,259 + ydeJ b1537 0.89 -0.4 1,623,297 + -0.7 1,623,297 + -0.0 1,623,309 - -0.8 1,623,309 - +0.1 1,623,411 - +0.2 1,623,449 + -0.0 1,623,449 + +0.6 1,623,501 + -1.1 1,623,519 - +0.0 1,623,739 + dcp b1538 0.19 -1.3 1,623,739 + dcp b1538 0.19 -0.4 1,623,752 - dcp b1538 0.19 -0.1 1,623,772 - dcp b1538 0.20 +0.2 1,623,772 - dcp b1538 0.20 +0.9 1,623,801 + dcp b1538 0.22 -0.5 1,623,844 - dcp b1538 0.24 +0.2 1,623,892 - dcp b1538 0.26 -0.2 1,623,951 + dcp b1538 0.29 -0.4 1,624,060 - dcp b1538 0.34 +0.4 1,624,089 + dcp b1538 0.36 +1.1 1,624,237 - dcp b1538 0.43 -0.1 1,624,297 - dcp b1538 0.46 -0.3
Or see this region's nucleotide sequence