Strain Fitness in Escherichia coli BW25113 around b2047

Experiment: LB + ciprofloxacin Time1-2hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwzxC and wcaJ are separated by 1 nucleotideswcaJ and cpsG are separated by 54 nucleotides b2046: wzxC - colanic acid exporter (NCBI), at 2,116,704 to 2,118,182 wzxC b2047: wcaJ - predicted UDP-glucose lipid carrier transferase (NCBI), at 2,118,184 to 2,119,578 wcaJ b2048: cpsG - phosphomannomutase (NCBI), at 2,119,633 to 2,121,003 cpsG Position (kb) 2118 2119 2120Strain fitness (log2 ratio) -1 0 1at 2117.196 kb on - strand, within wzxCat 2117.246 kb on + strand, within wzxCat 2117.262 kb on - strand, within wzxCat 2117.262 kb on - strand, within wzxCat 2117.272 kb on + strand, within wzxCat 2117.301 kb on + strand, within wzxCat 2117.431 kb on + strand, within wzxCat 2117.652 kb on - strand, within wzxCat 2117.652 kb on - strand, within wzxCat 2117.721 kb on + strand, within wzxCat 2117.877 kb on + strand, within wzxCat 2117.877 kb on + strand, within wzxCat 2118.014 kb on - strand, within wzxCat 2118.054 kb on - strandat 2118.054 kb on - strandat 2118.126 kb on - strandat 2118.353 kb on + strand, within wcaJat 2118.353 kb on + strand, within wcaJat 2118.391 kb on - strand, within wcaJat 2118.476 kb on - strand, within wcaJat 2119.085 kb on - strand, within wcaJat 2119.106 kb on - strand, within wcaJat 2119.436 kb on - strand, within wcaJat 2119.436 kb on - strand, within wcaJat 2119.439 kb on + strandat 2119.439 kb on + strandat 2119.476 kb on + strandat 2119.520 kb on + strandat 2119.536 kb on - strandat 2119.599 kb on + strandat 2119.796 kb on + strand, within cpsGat 2119.959 kb on + strand, within cpsGat 2120.189 kb on + strand, within cpsG

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time1-2hr
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2,117,196 - wzxC b2046 0.33 +0.4
2,117,246 + wzxC b2046 0.37 -0.7
2,117,262 - wzxC b2046 0.38 +0.5
2,117,262 - wzxC b2046 0.38 -1.3
2,117,272 + wzxC b2046 0.38 -0.0
2,117,301 + wzxC b2046 0.40 +0.1
2,117,431 + wzxC b2046 0.49 +0.1
2,117,652 - wzxC b2046 0.64 -0.1
2,117,652 - wzxC b2046 0.64 -0.3
2,117,721 + wzxC b2046 0.69 +0.1
2,117,877 + wzxC b2046 0.79 -0.7
2,117,877 + wzxC b2046 0.79 +0.4
2,118,014 - wzxC b2046 0.89 +0.3
2,118,054 - -0.0
2,118,054 - -0.1
2,118,126 - +0.1
2,118,353 + wcaJ b2047 0.12 -0.1
2,118,353 + wcaJ b2047 0.12 -0.5
2,118,391 - wcaJ b2047 0.15 -0.1
2,118,476 - wcaJ b2047 0.21 -0.0
2,119,085 - wcaJ b2047 0.65 -0.6
2,119,106 - wcaJ b2047 0.66 -0.5
2,119,436 - wcaJ b2047 0.90 -0.1
2,119,436 - wcaJ b2047 0.90 -1.4
2,119,439 + -0.2
2,119,439 + -0.7
2,119,476 + +0.7
2,119,520 + -0.1
2,119,536 - -0.6
2,119,599 + -0.1
2,119,796 + cpsG b2048 0.12 +0.2
2,119,959 + cpsG b2048 0.24 -0.2
2,120,189 + cpsG b2048 0.41 -0.1

Or see this region's nucleotide sequence