Experiment: LB + ciprofloxacin Time1-2hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt codA and cynR are separated by 336 nucleotides cynR and cynT are separated by 108 nucleotides cynT and cynS are separated by 30 nucleotides
b0337: codA - cytosine deaminase (NCBI), at 355,395 to 356,678
codA
b0338: cynR - DNA-binding transcriptional dual regulator (NCBI), at 357,015 to 357,914
cynR
b0339: cynT - carbonic anhydrase (NCBI), at 358,023 to 358,682
cynT
b0340: cynS - cyanate hydratase (NCBI), at 358,713 to 359,183
cynS
Position (kb) 357
358 Strain fitness (log2 ratio) -1
0
1 at 356.088 kb on + strand, within codA at 356.150 kb on + strand, within codA at 356.153 kb on + strand, within codA at 356.178 kb on - strand, within codA at 356.295 kb on - strand, within codA at 356.295 kb on - strand, within codA at 356.306 kb on - strand, within codA at 356.342 kb on - strand, within codA at 356.390 kb on + strand, within codA at 356.390 kb on + strand, within codA at 356.467 kb on + strand, within codA at 356.704 kb on - strand at 356.743 kb on - strand at 356.743 kb on - strand at 356.748 kb on - strand at 356.774 kb on - strand at 356.833 kb on + strand at 356.850 kb on + strand at 356.850 kb on + strand at 356.880 kb on + strand at 356.880 kb on + strand at 356.966 kb on + strand at 356.966 kb on + strand at 357.071 kb on - strand at 357.071 kb on - strand at 357.271 kb on - strand, within cynR at 357.274 kb on - strand, within cynR at 357.274 kb on - strand, within cynR at 357.386 kb on - strand, within cynR at 357.661 kb on - strand, within cynR at 357.720 kb on + strand, within cynR at 357.787 kb on + strand, within cynR at 357.827 kb on + strand at 357.849 kb on + strand at 357.849 kb on + strand at 357.880 kb on - strand at 357.880 kb on - strand at 357.910 kb on - strand at 358.023 kb on - strand at 358.077 kb on + strand at 358.087 kb on + strand at 358.103 kb on - strand, within cynT at 358.206 kb on - strand, within cynT at 358.206 kb on - strand, within cynT at 358.350 kb on + strand, within cynT at 358.386 kb on - strand, within cynT at 358.548 kb on + strand, within cynT at 358.562 kb on + strand, within cynT at 358.630 kb on + strand at 358.757 kb on - strand at 358.912 kb on - strand, within cynS at 358.912 kb on - strand, within cynS
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time1-2hr remove 356,088 + codA b0337 0.54 -0.4 356,150 + codA b0337 0.59 -0.3 356,153 + codA b0337 0.59 +0.8 356,178 - codA b0337 0.61 +0.2 356,295 - codA b0337 0.70 +0.5 356,295 - codA b0337 0.70 -0.2 356,306 - codA b0337 0.71 -0.2 356,342 - codA b0337 0.74 +0.1 356,390 + codA b0337 0.77 +0.2 356,390 + codA b0337 0.77 -0.0 356,467 + codA b0337 0.83 -0.4 356,704 - -0.1 356,743 - +0.5 356,743 - -0.2 356,748 - -0.4 356,774 - +0.5 356,833 + -0.5 356,850 + +0.2 356,850 + +0.1 356,880 + -0.0 356,880 + -0.3 356,966 + -0.4 356,966 + -0.8 357,071 - -0.3 357,071 - -0.0 357,271 - cynR b0338 0.28 -0.4 357,274 - cynR b0338 0.29 -0.4 357,274 - cynR b0338 0.29 -0.4 357,386 - cynR b0338 0.41 -0.4 357,661 - cynR b0338 0.72 -0.0 357,720 + cynR b0338 0.78 -0.5 357,787 + cynR b0338 0.86 -0.5 357,827 + +0.2 357,849 + +0.3 357,849 + +0.5 357,880 - +0.7 357,880 - -0.3 357,910 - -0.7 358,023 - -0.2 358,077 + -0.0 358,087 + +0.4 358,103 - cynT b0339 0.12 +1.0 358,206 - cynT b0339 0.28 +0.0 358,206 - cynT b0339 0.28 +0.0 358,350 + cynT b0339 0.50 +0.1 358,386 - cynT b0339 0.55 -0.3 358,548 + cynT b0339 0.80 -0.0 358,562 + cynT b0339 0.82 +0.4 358,630 + +0.0 358,757 - -0.2 358,912 - cynS b0340 0.42 +0.3 358,912 - cynS b0340 0.42 -0.3
Or see this region's nucleotide sequence