Strain Fitness in Escherichia coli BW25113 around b1961

Experiment: LB + carbenicillin Time7hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyedA and vsr overlap by 12 nucleotidesvsr and dcm overlap by 20 nucleotidesdcm and yedJ are separated by 66 nucleotidesyedJ and b1963 are separated by 39 nucleotides b1959: yedA - predicted inner membrane protein (NCBI), at 2,027,563 to 2,028,483 yedA b1960: vsr - DNA mismatch endonuclease of very short patch repair (NCBI), at 2,028,472 to 2,028,942 vsr b1961: dcm - DNA cytosine methylase (NCBI), at 2,028,923 to 2,030,341 dcm b1962: yedJ - predicted phosphohydrolase (NCBI), at 2,030,408 to 2,031,103 yedJ b1963: b1963 - orf, hypothetical protein (VIMSS), at 2,031,143 to 2,031,508 b1963 Position (kb) 2028 2029 2030 2031Strain fitness (log2 ratio) -1 0 1at 2028.038 kb on + strand, within yedAat 2028.081 kb on - strand, within yedAat 2028.322 kb on + strand, within yedAat 2028.398 kb on + strandat 2028.717 kb on + strand, within vsrat 2028.727 kb on - strand, within vsrat 2028.727 kb on - strand, within vsrat 2028.753 kb on + strand, within vsrat 2028.753 kb on + strand, within vsrat 2028.819 kb on - strand, within vsrat 2028.819 kb on - strand, within vsrat 2028.943 kb on + strandat 2028.966 kb on + strandat 2028.966 kb on - strandat 2029.342 kb on + strandat 2029.361 kb on - strand, within dcmat 2030.164 kb on + strand, within dcmat 2030.226 kb on + strandat 2030.226 kb on + strandat 2030.231 kb on + strandat 2030.451 kb on + strandat 2030.564 kb on + strand, within yedJat 2030.595 kb on + strand, within yedJat 2030.690 kb on + strand, within yedJat 2030.849 kb on + strand, within yedJat 2031.000 kb on + strand, within yedJat 2031.033 kb on - strand, within yedJat 2031.117 kb on + strandat 2031.157 kb on + strandat 2031.157 kb on + strandat 2031.174 kb on + strandat 2031.179 kb on + strandat 2031.179 kb on + strandat 2031.181 kb on - strand, within b1963at 2031.181 kb on - strand, within b1963at 2031.226 kb on + strand, within b1963at 2031.226 kb on + strand, within b1963at 2031.244 kb on + strand, within b1963

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time7hr
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2,028,038 + yedA b1959 0.52 -0.4
2,028,081 - yedA b1959 0.56 -0.2
2,028,322 + yedA b1959 0.82 +0.1
2,028,398 + +0.2
2,028,717 + vsr b1960 0.52 -0.8
2,028,727 - vsr b1960 0.54 -0.1
2,028,727 - vsr b1960 0.54 +0.1
2,028,753 + vsr b1960 0.60 +0.0
2,028,753 + vsr b1960 0.60 +0.3
2,028,819 - vsr b1960 0.74 +0.8
2,028,819 - vsr b1960 0.74 +0.4
2,028,943 + -0.2
2,028,966 + -0.2
2,028,966 - +0.2
2,029,342 + +1.4
2,029,361 - dcm b1961 0.31 +0.1
2,030,164 + dcm b1961 0.87 +0.7
2,030,226 + -0.4
2,030,226 + +0.4
2,030,231 + +0.1
2,030,451 + -0.1
2,030,564 + yedJ b1962 0.22 +0.2
2,030,595 + yedJ b1962 0.27 +0.2
2,030,690 + yedJ b1962 0.41 +0.1
2,030,849 + yedJ b1962 0.63 +0.5
2,031,000 + yedJ b1962 0.85 +0.3
2,031,033 - yedJ b1962 0.90 -0.1
2,031,117 + +0.0
2,031,157 + +0.5
2,031,157 + -0.2
2,031,174 + -0.1
2,031,179 + +0.3
2,031,179 + +0.1
2,031,181 - b1963 b1963 0.10 +0.1
2,031,181 - b1963 b1963 0.10 +0.1
2,031,226 + b1963 b1963 0.23 +0.1
2,031,226 + b1963 b1963 0.23 +0.2
2,031,244 + b1963 b1963 0.28 +0.6

Or see this region's nucleotide sequence