Experiment: LB + carbenicillin Time7hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hlyE and umuD are separated by 372 nucleotides umuD and umuC overlap by 1 nucleotides umuC and dsbB are separated by 45 nucleotides dsbB and nhaB are separated by 145 nucleotides
b1182: hlyE - hemolysin E (VIMSS), at 1,228,706 to 1,229,617
hlyE
b1183: umuD - DNA polymerase V, subunit D (NCBI), at 1,229,990 to 1,230,409
umuD
b1184: umuC - DNA polymerase V subunit UmuC (NCBI), at 1,230,409 to 1,231,677
umuC
b1185: dsbB - reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS), at 1,231,723 to 1,232,253
dsbB
b1186: nhaB - NhaB sodium/proton transporter (Katherine Huang), at 1,232,399 to 1,233,940
nhaB
Position (kb)
1230
1231
1232 Strain fitness (log2 ratio)
-1
0
1
2 at 1229.669 kb on - strand at 1229.703 kb on - strand at 1229.704 kb on - strand at 1229.718 kb on - strand at 1229.718 kb on - strand at 1229.838 kb on - strand at 1229.840 kb on - strand at 1229.842 kb on - strand at 1229.842 kb on - strand at 1229.844 kb on - strand at 1229.851 kb on - strand at 1229.851 kb on - strand at 1229.853 kb on - strand at 1229.859 kb on - strand at 1229.859 kb on - strand at 1229.859 kb on - strand at 1229.861 kb on + strand at 1229.897 kb on - strand at 1229.897 kb on - strand at 1230.140 kb on + strand, within umuD at 1230.307 kb on + strand, within umuD at 1230.446 kb on + strand at 1230.893 kb on + strand, within umuC at 1230.956 kb on + strand, within umuC at 1231.013 kb on - strand, within umuC at 1231.029 kb on + strand, within umuC at 1231.038 kb on - strand, within umuC at 1231.109 kb on + strand, within umuC at 1231.109 kb on + strand, within umuC at 1231.208 kb on + strand at 1231.208 kb on + strand, within umuC at 1231.270 kb on + strand, within umuC at 1231.270 kb on + strand, within umuC at 1231.320 kb on + strand, within umuC at 1231.359 kb on + strand, within umuC at 1231.359 kb on + strand, within umuC at 1231.382 kb on - strand, within umuC at 1231.457 kb on + strand, within umuC at 1231.519 kb on - strand, within umuC at 1231.550 kb on + strand, within umuC at 1231.551 kb on - strand at 1231.553 kb on - strand at 1231.573 kb on - strand at 1231.597 kb on + strand at 1231.625 kb on + strand at 1231.627 kb on - strand at 1231.643 kb on - strand at 1231.645 kb on - strand at 1231.660 kb on - strand at 1231.961 kb on + strand, within dsbB at 1231.961 kb on + strand, within dsbB at 1231.979 kb on - strand, within dsbB at 1232.074 kb on + strand, within dsbB at 1232.074 kb on + strand, within dsbB at 1232.077 kb on - strand, within dsbB at 1232.272 kb on + strand at 1232.272 kb on + strand at 1232.504 kb on - strand at 1232.509 kb on - strand at 1232.525 kb on + strand at 1232.569 kb on - strand, within nhaB
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time7hr remove 1,229,669 - +0.7 1,229,703 - +2.3 1,229,704 - -0.1 1,229,718 - +0.7 1,229,718 - +2.3 1,229,838 - -0.2 1,229,840 - -0.1 1,229,842 - -0.1 1,229,842 - +0.6 1,229,844 - +0.8 1,229,851 - +0.2 1,229,851 - +0.1 1,229,853 - -0.1 1,229,859 - -0.0 1,229,859 - +0.9 1,229,859 - -0.3 1,229,861 + -0.9 1,229,897 - +0.7 1,229,897 - +0.3 1,230,140 + umuD b1183 0.36 +0.2 1,230,307 + umuD b1183 0.75 -0.7 1,230,446 + +0.4 1,230,893 + umuC b1184 0.38 +0.1 1,230,956 + umuC b1184 0.43 +0.3 1,231,013 - umuC b1184 0.48 +1.0 1,231,029 + umuC b1184 0.49 +0.5 1,231,038 - umuC b1184 0.50 +0.3 1,231,109 + umuC b1184 0.55 +0.3 1,231,109 + umuC b1184 0.55 +0.0 1,231,208 + +2.5 1,231,208 + umuC b1184 0.63 +0.4 1,231,270 + umuC b1184 0.68 +0.3 1,231,270 + umuC b1184 0.68 +0.2 1,231,320 + umuC b1184 0.72 +0.5 1,231,359 + umuC b1184 0.75 +0.6 1,231,359 + umuC b1184 0.75 +0.4 1,231,382 - umuC b1184 0.77 +0.5 1,231,457 + umuC b1184 0.83 +0.1 1,231,519 - umuC b1184 0.87 +0.4 1,231,550 + umuC b1184 0.90 -0.1 1,231,551 - +0.6 1,231,553 - -0.7 1,231,573 - +0.3 1,231,597 + +0.1 1,231,625 + +0.0 1,231,627 - +0.4 1,231,643 - +1.0 1,231,645 - +0.6 1,231,660 - +0.1 1,231,961 + dsbB b1185 0.45 +1.4 1,231,961 + dsbB b1185 0.45 +1.0 1,231,979 - dsbB b1185 0.48 +1.5 1,232,074 + dsbB b1185 0.66 +1.2 1,232,074 + dsbB b1185 0.66 +0.9 1,232,077 - dsbB b1185 0.67 +1.9 1,232,272 + +0.4 1,232,272 + -0.1 1,232,504 - +0.3 1,232,509 - +0.1 1,232,525 + -0.0 1,232,569 - nhaB b1186 0.11 +0.4
Or see this region's nucleotide sequence