Strain Fitness in Escherichia coli BW25113 around b0178

Experiment: LB + carbenicillin Time7hr

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntyaeT and hlpA are separated by 121 nucleotideshlpA and lpxD are separated by 3 nucleotides b0177: yaeT - hypothetical protein (NCBI), at 197,928 to 200,360 yaeT b0178: hlpA - periplasmic chaperone (NCBI), at 200,482 to 200,967 hlpA b0179: lpxD - UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase (NCBI), at 200,971 to 201,996 lpxD Position (kb) 200 201Strain fitness (log2 ratio) -1 0 1at 200.394 kb on + strandat 200.394 kb on + strandat 200.394 kb on + strandat 200.414 kb on + strandat 200.414 kb on + strandat 200.439 kb on + strandat 200.523 kb on + strandat 200.523 kb on + strandat 200.635 kb on + strand, within hlpAat 200.635 kb on + strand, within hlpAat 200.649 kb on + strand, within hlpAat 200.785 kb on + strand, within hlpAat 200.834 kb on + strand, within hlpAat 200.903 kb on + strand, within hlpAat 200.903 kb on + strand, within hlpAat 200.924 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time7hr
remove
200,394 + -0.4
200,394 + -0.1
200,394 + +0.5
200,414 + +0.6
200,414 + +0.3
200,439 + +0.2
200,523 + +0.7
200,523 + +0.1
200,635 + hlpA b0178 0.31 +0.3
200,635 + hlpA b0178 0.31 +0.3
200,649 + hlpA b0178 0.34 +0.2
200,785 + hlpA b0178 0.62 +0.1
200,834 + hlpA b0178 0.72 +0.5
200,903 + hlpA b0178 0.87 +1.3
200,903 + hlpA b0178 0.87 +0.3
200,924 + +0.6

Or see this region's nucleotide sequence