Experiment: LB + ciprofloxacin Time4hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiA and plsB are separated by 154 nucleotides plsB and dgkA are separated by 170 nucleotides dgkA and lexA are separated by 109 nucleotides
b4040: ubiA - 4-hydroxybenzoate octaprenyltransferase (NCBI), at 4,251,039 to 4,251,911
ubiA
b4041: plsB - glycerol-3-phosphate acyltransferase (VIMSS), at 4,252,066 to 4,254,489
plsB
b4042: dgkA - diacylglycerol kinase (NCBI), at 4,254,660 to 4,255,028
dgkA
b4043: lexA - LexA repressor (NCBI), at 4,255,138 to 4,255,746
lexA
Position (kb)
4252
4253
4254
4255 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4251.913 kb on + strand at 4251.953 kb on - strand at 4251.972 kb on + strand at 4251.980 kb on - strand at 4251.980 kb on - strand at 4251.985 kb on - strand at 4251.985 kb on - strand at 4252.063 kb on + strand at 4252.063 kb on + strand at 4252.069 kb on + strand at 4252.096 kb on - strand at 4252.104 kb on - strand at 4252.105 kb on - strand at 4252.105 kb on - strand at 4252.364 kb on - strand, within plsB at 4254.489 kb on - strand at 4254.503 kb on - strand at 4254.508 kb on + strand at 4254.508 kb on + strand at 4254.537 kb on - strand at 4254.568 kb on - strand at 4254.588 kb on - strand at 4254.608 kb on + strand at 4254.608 kb on + strand at 4254.618 kb on + strand at 4254.623 kb on + strand at 4254.623 kb on + strand at 4254.626 kb on + strand at 4254.629 kb on - strand at 4254.629 kb on - strand at 4254.681 kb on - strand at 4254.738 kb on - strand, within dgkA at 4254.745 kb on - strand, within dgkA at 4254.745 kb on - strand, within dgkA at 4254.782 kb on - strand, within dgkA at 4254.817 kb on + strand, within dgkA at 4254.824 kb on - strand, within dgkA at 4254.831 kb on + strand, within dgkA at 4254.833 kb on + strand, within dgkA at 4254.870 kb on - strand, within dgkA at 4254.912 kb on + strand, within dgkA at 4255.118 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time4hr remove 4,251,913 + +0.4 4,251,953 - -0.3 4,251,972 + -0.1 4,251,980 - +0.2 4,251,980 - +0.5 4,251,985 - +0.3 4,251,985 - +1.0 4,252,063 + +1.5 4,252,063 + +0.4 4,252,069 + +0.0 4,252,096 - +0.2 4,252,104 - -2.6 4,252,105 - -0.2 4,252,105 - -1.2 4,252,364 - plsB b4041 0.12 -4.6 4,254,489 - +1.2 4,254,503 - -2.1 4,254,508 + -2.5 4,254,508 + -4.9 4,254,537 - +0.3 4,254,568 - +0.2 4,254,588 - +0.2 4,254,608 + +0.6 4,254,608 + +0.1 4,254,618 + +0.2 4,254,623 + +0.7 4,254,623 + -0.3 4,254,626 + +0.2 4,254,629 - -1.1 4,254,629 - +0.1 4,254,681 - +2.2 4,254,738 - dgkA b4042 0.21 +1.2 4,254,745 - dgkA b4042 0.23 +1.2 4,254,745 - dgkA b4042 0.23 +1.2 4,254,782 - dgkA b4042 0.33 +1.2 4,254,817 + dgkA b4042 0.43 +1.2 4,254,824 - dgkA b4042 0.44 +1.2 4,254,831 + dgkA b4042 0.46 +1.2 4,254,833 + dgkA b4042 0.47 +1.2 4,254,870 - dgkA b4042 0.57 +1.2 4,254,912 + dgkA b4042 0.68 +1.2 4,255,118 + +0.2
Or see this region's nucleotide sequence