Experiment: LB + ciprofloxacin Time4hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recJ and dsbC are separated by 5 nucleotides dsbC and xerD are separated by 24 nucleotides xerD and fldB are separated by 111 nucleotides fldB and ygfX are separated by 39 nucleotides
b2892: recJ - ssDNA exonuclease, 5' --> 3'-specific (NCBI), at 3,034,395 to 3,036,128
recJ
b2893: dsbC - protein disulfide isomerase II (NCBI), at 3,036,134 to 3,036,844
dsbC
b2894: xerD - site-specific tyrosine recombinase XerD (NCBI), at 3,036,869 to 3,037,765
xerD
b2895: fldB - flavodoxin 2 (NCBI), at 3,037,877 to 3,038,398
fldB
b2896: ygfX - hypothetical protein (NCBI), at 3,038,438 to 3,038,845
ygfX
Position (kb)
3036
3037
3038 Strain fitness (log2 ratio)
-1
0
1 at 3035.869 kb on - strand, within recJ at 3035.906 kb on - strand, within recJ at 3035.906 kb on - strand at 3035.960 kb on - strand at 3035.996 kb on - strand at 3035.996 kb on - strand at 3035.996 kb on - strand at 3036.046 kb on - strand at 3036.068 kb on - strand at 3036.229 kb on + strand, within dsbC at 3036.229 kb on - strand, within dsbC at 3036.268 kb on + strand, within dsbC at 3036.457 kb on - strand, within dsbC at 3036.511 kb on + strand, within dsbC at 3036.511 kb on + strand, within dsbC at 3036.613 kb on - strand, within dsbC at 3036.626 kb on - strand, within dsbC at 3036.660 kb on - strand, within dsbC at 3036.660 kb on - strand, within dsbC at 3036.666 kb on + strand, within dsbC at 3036.668 kb on + strand, within dsbC at 3036.761 kb on + strand, within dsbC at 3037.372 kb on + strand, within xerD at 3037.773 kb on + strand at 3037.777 kb on + strand at 3037.777 kb on + strand at 3037.900 kb on + strand at 3037.900 kb on + strand at 3037.986 kb on - strand, within fldB at 3038.097 kb on + strand, within fldB at 3038.128 kb on + strand, within fldB at 3038.128 kb on + strand, within fldB at 3038.128 kb on + strand, within fldB at 3038.149 kb on + strand, within fldB at 3038.149 kb on + strand, within fldB at 3038.321 kb on + strand, within fldB at 3038.348 kb on - strand at 3038.348 kb on - strand at 3038.418 kb on - strand at 3038.516 kb on - strand, within ygfX at 3038.698 kb on - strand, within ygfX at 3038.714 kb on + strand, within ygfX at 3038.714 kb on + strand, within ygfX at 3038.716 kb on - strand, within ygfX at 3038.718 kb on + strand, within ygfX at 3038.718 kb on + strand, within ygfX at 3038.746 kb on - strand, within ygfX
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time4hr remove 3,035,869 - recJ b2892 0.85 +0.3 3,035,906 - recJ b2892 0.87 +0.2 3,035,906 - -0.5 3,035,960 - -0.2 3,035,996 - -0.1 3,035,996 - -0.6 3,035,996 - +0.3 3,036,046 - -0.3 3,036,068 - -0.7 3,036,229 + dsbC b2893 0.13 -0.2 3,036,229 - dsbC b2893 0.13 +0.2 3,036,268 + dsbC b2893 0.19 +0.1 3,036,457 - dsbC b2893 0.45 +0.3 3,036,511 + dsbC b2893 0.53 +0.1 3,036,511 + dsbC b2893 0.53 +0.5 3,036,613 - dsbC b2893 0.67 +0.1 3,036,626 - dsbC b2893 0.69 +0.6 3,036,660 - dsbC b2893 0.74 -0.1 3,036,660 - dsbC b2893 0.74 +0.7 3,036,666 + dsbC b2893 0.75 -0.1 3,036,668 + dsbC b2893 0.75 +0.6 3,036,761 + dsbC b2893 0.88 +0.8 3,037,372 + xerD b2894 0.56 -1.6 3,037,773 + +0.3 3,037,777 + +0.0 3,037,777 + -0.2 3,037,900 + +0.3 3,037,900 + +0.8 3,037,986 - fldB b2895 0.21 +0.0 3,038,097 + fldB b2895 0.42 +0.5 3,038,128 + fldB b2895 0.48 -0.9 3,038,128 + fldB b2895 0.48 +0.8 3,038,128 + fldB b2895 0.48 +0.7 3,038,149 + fldB b2895 0.52 -0.1 3,038,149 + fldB b2895 0.52 -0.4 3,038,321 + fldB b2895 0.85 +0.6 3,038,348 - +0.3 3,038,348 - -0.3 3,038,418 - +0.1 3,038,516 - ygfX b2896 0.19 +0.1 3,038,698 - ygfX b2896 0.64 -0.0 3,038,714 + ygfX b2896 0.68 +0.3 3,038,714 + ygfX b2896 0.68 +0.3 3,038,716 - ygfX b2896 0.68 +0.7 3,038,718 + ygfX b2896 0.69 +0.1 3,038,718 + ygfX b2896 0.69 +0.0 3,038,746 - ygfX b2896 0.75 +0.2
Or see this region's nucleotide sequence