Experiment: LB + ciprofloxacin Time4hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hmp and glnB are separated by 44 nucleotides glnB and yfhA are separated by 60 nucleotides
b2552: hmp - fused nitric oxide dioxygenase/dihydropteridine reductase 2 (NCBI), at 2,683,857 to 2,685,047
hmp
b2553: glnB - regulatory protein P-II for glutamine synthetase (NCBI), at 2,685,092 to 2,685,430
glnB
b2554: yfhA - predicted DNA-binding response regulator in two-component system (NCBI), at 2,685,491 to 2,686,825
yfhA
Position (kb)
2685
2686 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2684.113 kb on - strand, within hmp at 2684.136 kb on + strand, within hmp at 2684.136 kb on + strand, within hmp at 2684.146 kb on - strand, within hmp at 2684.146 kb on - strand, within hmp at 2684.146 kb on - strand, within hmp at 2684.146 kb on - strand, within hmp at 2684.160 kb on - strand, within hmp at 2684.160 kb on - strand, within hmp at 2684.191 kb on - strand, within hmp at 2684.191 kb on - strand, within hmp at 2684.207 kb on + strand, within hmp at 2684.222 kb on + strand, within hmp at 2684.222 kb on + strand, within hmp at 2684.330 kb on + strand, within hmp at 2684.338 kb on - strand, within hmp at 2684.338 kb on - strand, within hmp at 2684.405 kb on - strand, within hmp at 2684.476 kb on - strand, within hmp at 2684.721 kb on + strand, within hmp at 2684.789 kb on + strand, within hmp at 2684.789 kb on + strand, within hmp at 2684.827 kb on - strand, within hmp at 2684.830 kb on - strand, within hmp at 2684.970 kb on + strand at 2684.970 kb on + strand at 2685.032 kb on + strand at 2685.047 kb on - strand at 2685.079 kb on + strand at 2685.107 kb on + strand at 2685.107 kb on + strand at 2685.120 kb on + strand at 2685.120 kb on + strand at 2685.223 kb on - strand, within glnB at 2685.273 kb on + strand, within glnB at 2685.273 kb on + strand, within glnB at 2685.281 kb on - strand, within glnB at 2685.288 kb on + strand, within glnB at 2685.311 kb on - strand, within glnB at 2685.311 kb on - strand, within glnB at 2685.311 kb on - strand, within glnB at 2685.311 kb on - strand, within glnB at 2685.371 kb on - strand, within glnB at 2685.488 kb on + strand at 2685.488 kb on + strand at 2685.525 kb on - strand at 2685.682 kb on + strand, within yfhA at 2685.703 kb on + strand, within yfhA at 2685.703 kb on + strand, within yfhA at 2685.725 kb on + strand, within yfhA at 2685.821 kb on - strand, within yfhA at 2685.821 kb on - strand, within yfhA at 2685.863 kb on - strand, within yfhA at 2685.885 kb on - strand, within yfhA at 2685.885 kb on - strand, within yfhA at 2685.897 kb on - strand, within yfhA at 2686.064 kb on + strand, within yfhA at 2686.079 kb on + strand, within yfhA at 2686.089 kb on + strand at 2686.091 kb on + strand, within yfhA at 2686.091 kb on + strand, within yfhA at 2686.158 kb on - strand, within yfhA at 2686.162 kb on - strand, within yfhA at 2686.250 kb on + strand, within yfhA at 2686.282 kb on + strand, within yfhA at 2686.294 kb on - strand, within yfhA at 2686.323 kb on + strand, within yfhA at 2686.323 kb on + strand, within yfhA at 2686.331 kb on - strand, within yfhA at 2686.425 kb on - strand, within yfhA
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time4hr remove 2,684,113 - hmp b2552 0.21 +0.2 2,684,136 + hmp b2552 0.23 -0.2 2,684,136 + hmp b2552 0.23 +0.5 2,684,146 - hmp b2552 0.24 -0.3 2,684,146 - hmp b2552 0.24 +0.4 2,684,146 - hmp b2552 0.24 +0.5 2,684,146 - hmp b2552 0.24 +0.1 2,684,160 - hmp b2552 0.25 +0.7 2,684,160 - hmp b2552 0.25 +0.0 2,684,191 - hmp b2552 0.28 +0.2 2,684,191 - hmp b2552 0.28 +0.7 2,684,207 + hmp b2552 0.29 +0.4 2,684,222 + hmp b2552 0.31 -0.1 2,684,222 + hmp b2552 0.31 +0.2 2,684,330 + hmp b2552 0.40 +0.2 2,684,338 - hmp b2552 0.40 +0.3 2,684,338 - hmp b2552 0.40 -0.6 2,684,405 - hmp b2552 0.46 +0.2 2,684,476 - hmp b2552 0.52 +0.1 2,684,721 + hmp b2552 0.73 -0.5 2,684,789 + hmp b2552 0.78 +0.8 2,684,789 + hmp b2552 0.78 +0.1 2,684,827 - hmp b2552 0.81 +0.9 2,684,830 - hmp b2552 0.82 -0.2 2,684,970 + +0.6 2,684,970 + -0.3 2,685,032 + +0.5 2,685,047 - +0.4 2,685,079 + -0.1 2,685,107 + -0.5 2,685,107 + -0.0 2,685,120 + +0.1 2,685,120 + -0.2 2,685,223 - glnB b2553 0.39 +0.1 2,685,273 + glnB b2553 0.53 -0.2 2,685,273 + glnB b2553 0.53 +0.1 2,685,281 - glnB b2553 0.56 -2.3 2,685,288 + glnB b2553 0.58 -0.1 2,685,311 - glnB b2553 0.65 -1.2 2,685,311 - glnB b2553 0.65 -0.3 2,685,311 - glnB b2553 0.65 -2.3 2,685,311 - glnB b2553 0.65 +0.2 2,685,371 - glnB b2553 0.82 -0.7 2,685,488 + -0.1 2,685,488 + -0.0 2,685,525 - +0.8 2,685,682 + yfhA b2554 0.14 +0.3 2,685,703 + yfhA b2554 0.16 +0.4 2,685,703 + yfhA b2554 0.16 -0.4 2,685,725 + yfhA b2554 0.18 -0.7 2,685,821 - yfhA b2554 0.25 +0.1 2,685,821 - yfhA b2554 0.25 -1.0 2,685,863 - yfhA b2554 0.28 -0.2 2,685,885 - yfhA b2554 0.30 -0.1 2,685,885 - yfhA b2554 0.30 +0.5 2,685,897 - yfhA b2554 0.30 -0.1 2,686,064 + yfhA b2554 0.43 -0.2 2,686,079 + yfhA b2554 0.44 +0.1 2,686,089 + -0.8 2,686,091 + yfhA b2554 0.45 +2.0 2,686,091 + yfhA b2554 0.45 -0.3 2,686,158 - yfhA b2554 0.50 +0.7 2,686,162 - yfhA b2554 0.50 +0.1 2,686,250 + yfhA b2554 0.57 -0.1 2,686,282 + yfhA b2554 0.59 +0.1 2,686,294 - yfhA b2554 0.60 -0.7 2,686,323 + yfhA b2554 0.62 +0.2 2,686,323 + yfhA b2554 0.62 -0.0 2,686,331 - yfhA b2554 0.63 +0.3 2,686,425 - yfhA b2554 0.70 +0.5
Or see this region's nucleotide sequence