Experiment: LB + ciprofloxacin Time4hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potD and potC overlap by 4 nucleotides potC and potB overlap by 4 nucleotides potB and potA are separated by 13 nucleotides
b1123: potD - spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI), at 1,181,006 to 1,182,052
potD
b1124: potC - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,049 to 1,182,843
potC
b1125: potB - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,840 to 1,183,667
potB
b1126: potA - putrescine/spermidine ABC transporter ATPase protein (NCBI), at 1,183,681 to 1,184,817
potA
Position (kb)
1182
1183
1184 Strain fitness (log2 ratio)
-2
-1
0
1 at 1181.865 kb on - strand, within potD at 1181.918 kb on + strand, within potD at 1182.003 kb on + strand at 1182.395 kb on + strand, within potC at 1182.474 kb on - strand, within potC at 1182.476 kb on - strand, within potC at 1182.524 kb on + strand, within potC at 1182.581 kb on + strand, within potC at 1182.581 kb on + strand, within potC at 1182.589 kb on + strand, within potC at 1182.668 kb on + strand, within potC at 1182.691 kb on + strand, within potC at 1182.700 kb on + strand, within potC at 1182.730 kb on + strand, within potC at 1182.910 kb on + strand at 1182.910 kb on + strand at 1182.914 kb on - strand at 1183.032 kb on + strand, within potB at 1183.032 kb on + strand, within potB at 1183.193 kb on - strand, within potB at 1183.202 kb on + strand, within potB at 1183.202 kb on + strand, within potB at 1183.283 kb on + strand, within potB at 1183.449 kb on + strand, within potB at 1183.542 kb on - strand, within potB at 1183.680 kb on - strand at 1183.698 kb on - strand at 1183.698 kb on - strand at 1183.711 kb on - strand at 1183.711 kb on - strand at 1183.884 kb on + strand, within potA at 1183.934 kb on - strand, within potA at 1184.015 kb on + strand, within potA at 1184.139 kb on - strand, within potA at 1184.163 kb on + strand, within potA at 1184.163 kb on + strand, within potA at 1184.286 kb on + strand, within potA at 1184.293 kb on + strand, within potA at 1184.334 kb on - strand, within potA at 1184.361 kb on - strand, within potA at 1184.599 kb on - strand, within potA at 1184.666 kb on + strand, within potA
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time4hr remove 1,181,865 - potD b1123 0.82 -0.3 1,181,918 + potD b1123 0.87 -1.3 1,182,003 + -1.3 1,182,395 + potC b1124 0.44 -0.5 1,182,474 - potC b1124 0.53 -1.1 1,182,476 - potC b1124 0.54 -1.5 1,182,524 + potC b1124 0.60 -1.2 1,182,581 + potC b1124 0.67 -1.4 1,182,581 + potC b1124 0.67 -0.7 1,182,589 + potC b1124 0.68 -1.0 1,182,668 + potC b1124 0.78 -1.7 1,182,691 + potC b1124 0.81 -0.5 1,182,700 + potC b1124 0.82 -1.6 1,182,730 + potC b1124 0.86 -1.7 1,182,910 + -0.5 1,182,910 + -1.0 1,182,914 - -0.4 1,183,032 + potB b1125 0.23 -0.4 1,183,032 + potB b1125 0.23 -1.0 1,183,193 - potB b1125 0.43 -1.4 1,183,202 + potB b1125 0.44 -0.8 1,183,202 + potB b1125 0.44 -1.5 1,183,283 + potB b1125 0.54 -0.6 1,183,449 + potB b1125 0.74 -0.8 1,183,542 - potB b1125 0.85 -0.9 1,183,680 - +0.1 1,183,698 - -0.2 1,183,698 - +0.7 1,183,711 - -1.1 1,183,711 - -0.7 1,183,884 + potA b1126 0.18 -1.5 1,183,934 - potA b1126 0.22 -1.1 1,184,015 + potA b1126 0.29 -1.5 1,184,139 - potA b1126 0.40 -1.3 1,184,163 + potA b1126 0.42 -1.3 1,184,163 + potA b1126 0.42 -1.4 1,184,286 + potA b1126 0.53 -0.3 1,184,293 + potA b1126 0.54 -2.1 1,184,334 - potA b1126 0.57 -1.1 1,184,361 - potA b1126 0.60 -1.0 1,184,599 - potA b1126 0.81 -1.4 1,184,666 + potA b1126 0.87 -1.4
Or see this region's nucleotide sequence