Experiment: LB + ciprofloxacin Time1-2hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ilvB and ivbL are separated by 105 nucleotides ivbL and istR are separated by 129 nucleotides istR and tisB are separated by 295 nucleotides tisB and emrD are separated by 279 nucleotides
b3671: ilvB - acetolactate synthase large subunit (NCBI), at 3,849,119 to 3,850,807
ilvB
b3672: ivbL - ilvB operon leader peptide (NCBI), at 3,850,913 to 3,851,011
ivbL
b4616: istR - sRNAs IstR-1 and IstR-2, tisB regulators (RefSeq), at 3,851,141 to 3,851,280
istR
b4618: tisB - lexA-regulated toxic peptide (RefSeq), at 3,851,576 to 3,851,665
tisB
b3673: emrD - 2-module integral membrane pump; multidrug resistance (VIMSS), at 3,851,945 to 3,853,129
emrD
Position (kb)
3851
3852 Strain fitness (log2 ratio)
-1
0
1 at 3850.627 kb on + strand, within ilvB at 3850.635 kb on - strand, within ilvB at 3850.661 kb on + strand at 3850.674 kb on - strand at 3850.674 kb on - strand at 3850.674 kb on - strand at 3850.674 kb on - strand at 3850.690 kb on + strand at 3850.690 kb on + strand at 3850.698 kb on - strand at 3850.709 kb on + strand at 3850.709 kb on + strand at 3850.775 kb on - strand at 3850.791 kb on + strand at 3850.803 kb on + strand at 3850.805 kb on - strand at 3850.805 kb on - strand at 3850.817 kb on - strand at 3850.898 kb on + strand at 3850.901 kb on - strand at 3850.903 kb on + strand at 3850.903 kb on + strand at 3850.911 kb on + strand at 3850.919 kb on - strand at 3850.919 kb on - strand at 3850.939 kb on - strand, within ivbL at 3850.965 kb on - strand, within ivbL at 3850.976 kb on + strand, within ivbL at 3850.976 kb on + strand, within ivbL at 3850.996 kb on + strand, within ivbL at 3850.996 kb on + strand, within ivbL at 3851.001 kb on + strand, within ivbL at 3851.001 kb on + strand, within ivbL at 3851.010 kb on + strand at 3851.010 kb on + strand at 3851.369 kb on + strand at 3851.369 kb on + strand at 3851.379 kb on - strand at 3851.379 kb on - strand at 3851.446 kb on - strand at 3851.446 kb on - strand at 3851.464 kb on - strand at 3851.469 kb on - strand at 3851.469 kb on - strand at 3851.469 kb on - strand at 3851.491 kb on + strand at 3851.493 kb on + strand at 3851.499 kb on - strand at 3851.514 kb on - strand at 3851.545 kb on + strand at 3851.545 kb on + strand at 3851.553 kb on + strand at 3851.592 kb on + strand, within tisB at 3851.605 kb on + strand, within tisB at 3851.627 kb on - strand, within tisB at 3851.644 kb on - strand, within tisB at 3851.709 kb on + strand at 3851.733 kb on + strand at 3851.733 kb on + strand at 3851.772 kb on - strand at 3851.849 kb on - strand at 3851.851 kb on - strand at 3851.977 kb on - strand at 3851.978 kb on + strand at 3851.978 kb on + strand at 3851.986 kb on - strand at 3851.990 kb on + strand at 3851.990 kb on + strand at 3851.997 kb on - strand at 3852.000 kb on - strand at 3852.056 kb on - strand at 3852.091 kb on + strand, within emrD at 3852.204 kb on + strand, within emrD at 3852.244 kb on - strand, within emrD at 3852.265 kb on + strand, within emrD at 3852.285 kb on + strand, within emrD at 3852.285 kb on + strand, within emrD at 3852.287 kb on + strand, within emrD at 3852.287 kb on + strand, within emrD at 3852.337 kb on - strand, within emrD at 3852.341 kb on + strand, within emrD at 3852.350 kb on - strand, within emrD at 3852.350 kb on - strand, within emrD at 3852.406 kb on + strand, within emrD at 3852.414 kb on + strand, within emrD at 3852.414 kb on - strand, within emrD at 3852.535 kb on - strand, within emrD at 3852.600 kb on - strand, within emrD at 3852.609 kb on + strand, within emrD at 3852.609 kb on + strand, within emrD at 3852.630 kb on + strand, within emrD at 3852.643 kb on + strand, within emrD at 3852.646 kb on - strand, within emrD at 3852.653 kb on + strand, within emrD at 3852.653 kb on + strand, within emrD at 3852.661 kb on - strand, within emrD at 3852.661 kb on - strand, within emrD at 3852.662 kb on - strand, within emrD at 3852.662 kb on - strand, within emrD at 3852.662 kb on - strand, within emrD at 3852.662 kb on - strand, within emrD at 3852.662 kb on - strand, within emrD at 3852.662 kb on - strand, within emrD at 3852.662 kb on - strand, within emrD at 3852.662 kb on - strand, within emrD
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time1-2hr remove 3,850,627 + ilvB b3671 0.89 +0.3 3,850,635 - ilvB b3671 0.90 -0.1 3,850,661 + -0.1 3,850,674 - +0.1 3,850,674 - +0.0 3,850,674 - -0.4 3,850,674 - -0.5 3,850,690 + +0.0 3,850,690 + +0.1 3,850,698 - -0.0 3,850,709 + +0.1 3,850,709 + +0.1 3,850,775 - +0.0 3,850,791 + -0.1 3,850,803 + -0.0 3,850,805 - -0.1 3,850,805 - +0.1 3,850,817 - +0.8 3,850,898 + +0.1 3,850,901 - -0.3 3,850,903 + -0.1 3,850,903 + +0.1 3,850,911 + +0.2 3,850,919 - +0.0 3,850,919 - +0.2 3,850,939 - ivbL b3672 0.26 -0.0 3,850,965 - ivbL b3672 0.53 +0.1 3,850,976 + ivbL b3672 0.64 -0.4 3,850,976 + ivbL b3672 0.64 -0.2 3,850,996 + ivbL b3672 0.84 +0.7 3,850,996 + ivbL b3672 0.84 -0.8 3,851,001 + ivbL b3672 0.89 +0.4 3,851,001 + ivbL b3672 0.89 -0.2 3,851,010 + -0.5 3,851,010 + +0.5 3,851,369 + -0.1 3,851,369 + -0.5 3,851,379 - +0.2 3,851,379 - -0.5 3,851,446 - -0.3 3,851,446 - -0.5 3,851,464 - +0.1 3,851,469 - -0.9 3,851,469 - +0.1 3,851,469 - -0.4 3,851,491 + -0.4 3,851,493 + +0.4 3,851,499 - -0.2 3,851,514 - +0.5 3,851,545 + +0.0 3,851,545 + -0.5 3,851,553 + +0.4 3,851,592 + tisB b4618 0.18 -0.0 3,851,605 + tisB b4618 0.32 +0.5 3,851,627 - tisB b4618 0.57 +0.3 3,851,644 - tisB b4618 0.76 -0.0 3,851,709 + -0.0 3,851,733 + -0.1 3,851,733 + -0.1 3,851,772 - +0.0 3,851,849 - -0.1 3,851,851 - +0.2 3,851,977 - -0.0 3,851,978 + -0.4 3,851,978 + -0.1 3,851,986 - -0.2 3,851,990 + -0.2 3,851,990 + +0.7 3,851,997 - +0.6 3,852,000 - +0.4 3,852,056 - -0.2 3,852,091 + emrD b3673 0.12 -0.0 3,852,204 + emrD b3673 0.22 -0.3 3,852,244 - emrD b3673 0.25 -0.1 3,852,265 + emrD b3673 0.27 -0.1 3,852,285 + emrD b3673 0.29 -0.1 3,852,285 + emrD b3673 0.29 -0.2 3,852,287 + emrD b3673 0.29 -0.2 3,852,287 + emrD b3673 0.29 -0.2 3,852,337 - emrD b3673 0.33 +0.1 3,852,341 + emrD b3673 0.33 -0.2 3,852,350 - emrD b3673 0.34 +0.5 3,852,350 - emrD b3673 0.34 +1.0 3,852,406 + emrD b3673 0.39 -0.3 3,852,414 + emrD b3673 0.40 -0.3 3,852,414 - emrD b3673 0.40 -0.4 3,852,535 - emrD b3673 0.50 -0.3 3,852,600 - emrD b3673 0.55 -0.6 3,852,609 + emrD b3673 0.56 -0.5 3,852,609 + emrD b3673 0.56 +0.3 3,852,630 + emrD b3673 0.58 -0.0 3,852,643 + emrD b3673 0.59 -0.2 3,852,646 - emrD b3673 0.59 +0.1 3,852,653 + emrD b3673 0.60 -0.4 3,852,653 + emrD b3673 0.60 +0.1 3,852,661 - emrD b3673 0.60 +0.1 3,852,661 - emrD b3673 0.60 -0.2 3,852,662 - emrD b3673 0.61 +0.5 3,852,662 - emrD b3673 0.61 +0.0 3,852,662 - emrD b3673 0.61 +0.3 3,852,662 - emrD b3673 0.61 +0.1 3,852,662 - emrD b3673 0.61 +0.3 3,852,662 - emrD b3673 0.61 -0.3 3,852,662 - emrD b3673 0.61 +0.0 3,852,662 - emrD b3673 0.61 +0.0
Or see this region's nucleotide sequence