Strain Fitness in Escherichia coli BW25113 around b1882

Experiment: LB + ciprofloxacin Time1-2hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflhB and cheZ are separated by 201 nucleotidescheZ and cheY are separated by 10 nucleotidescheY and cheB are separated by 14 nucleotides b1880: flhB - flagellar biosynthesis protein B (NCBI), at 1,963,067 to 1,964,215 flhB b1881: cheZ - chemotaxis regulator, protein phosphatase for CheY (NCBI), at 1,964,417 to 1,965,061 cheZ b1882: cheY - chemotaxis regulator transmitting signal to flagellar motor component (NCBI), at 1,965,072 to 1,965,461 cheY b1883: cheB - chemotaxis-specific methylesterase (NCBI), at 1,965,476 to 1,966,525 cheB Position (kb) 1965 1966Strain fitness (log2 ratio) -1 0 1at 1964.080 kb on + strand, within flhBat 1964.080 kb on + strand, within flhBat 1964.088 kb on + strand, within flhBat 1964.507 kb on - strand, within cheZat 1964.609 kb on - strand, within cheZat 1964.847 kb on - strand, within cheZat 1964.889 kb on + strand, within cheZat 1965.270 kb on + strand, within cheYat 1965.696 kb on - strand, within cheBat 1965.815 kb on - strand, within cheBat 1966.252 kb on + strand, within cheBat 1966.273 kb on + strand, within cheBat 1966.356 kb on - strand, within cheB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time1-2hr
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1,964,080 + flhB b1880 0.88 -0.2
1,964,080 + flhB b1880 0.88 +0.3
1,964,088 + flhB b1880 0.89 +0.1
1,964,507 - cheZ b1881 0.14 +0.7
1,964,609 - cheZ b1881 0.30 -0.1
1,964,847 - cheZ b1881 0.67 -0.4
1,964,889 + cheZ b1881 0.73 -0.2
1,965,270 + cheY b1882 0.51 +0.5
1,965,696 - cheB b1883 0.21 -0.0
1,965,815 - cheB b1883 0.32 -0.0
1,966,252 + cheB b1883 0.74 +0.0
1,966,273 + cheB b1883 0.76 +0.5
1,966,356 - cheB b1883 0.84 -0.1

Or see this region's nucleotide sequence