Strain Fitness in Escherichia coli BW25113 around b1573

Experiment: LB + ciprofloxacin Time1-2hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdicC and dicA are separated by 83 nucleotidesdicA and ydfA are separated by 166 nucleotidesydfA and ydfB are separated by 1 nucleotidesydfB and ydfC are separated by 29 nucleotidesydfC and dicF are separated by 340 nucleotidesdicF and dicB are separated by 174 nucleotidesdicB and ydfD overlap by 4 nucleotides b1569: dicC - Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI), at 1,645,644 to 1,645,874 dicC b1570: dicA - Qin prophage; predicted regulator for DicB (NCBI), at 1,645,958 to 1,646,365 dicA b1571: ydfA - Qin prophage; predicted protein (NCBI), at 1,646,532 to 1,646,687 ydfA b1572: ydfB - orf, hypothetical protein (VIMSS), at 1,646,689 to 1,646,817 ydfB b1573: ydfC - Qin prophage; predicted protein (RefSeq), at 1,646,847 to 1,647,065 ydfC b1574: dicF - antisense RNA DicF (NCBI), at 1,647,406 to 1,647,458 dicF b1575: dicB - Qin prophage; cell division inhibition protein (NCBI), at 1,647,633 to 1,647,821 dicB b1576: ydfD - Qin prophage; predicted protein (NCBI), at 1,647,818 to 1,648,009 ydfD Position (kb) 1646 1647 1648Strain fitness (log2 ratio) -1 0 1at 1645.940 kb on + strandat 1646.751 kb on - strand, within ydfBat 1646.887 kb on - strand, within ydfCat 1646.997 kb on - strand, within ydfCat 1647.366 kb on - strandat 1647.500 kb on - strandat 1647.653 kb on + strand, within dicBat 1647.700 kb on + strand, within dicBat 1647.709 kb on + strand, within dicBat 1647.775 kb on - strand, within dicBat 1647.880 kb on - strand, within ydfDat 1647.900 kb on - strand, within ydfDat 1647.957 kb on - strand, within ydfD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time1-2hr
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1,645,940 + -0.1
1,646,751 - ydfB b1572 0.48 +0.3
1,646,887 - ydfC b1573 0.18 +0.8
1,646,997 - ydfC b1573 0.68 -0.5
1,647,366 - -1.6
1,647,500 - -0.0
1,647,653 + dicB b1575 0.11 -0.4
1,647,700 + dicB b1575 0.35 -0.0
1,647,709 + dicB b1575 0.40 +1.0
1,647,775 - dicB b1575 0.75 +1.0
1,647,880 - ydfD b1576 0.32 +1.0
1,647,900 - ydfD b1576 0.43 +1.0
1,647,957 - ydfD b1576 0.72 +1.0

Or see this region's nucleotide sequence