Experiment: LB + ciprofloxacin Time1-2hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydaW and rzpR are separated by 0 nucleotides rzpR and rzoR overlap by 245 nucleotides rzoR and trkG are separated by 196 nucleotides trkG and ynaK are separated by 137 nucleotides ynaK and ydaY overlap by 11 nucleotides
b1361: ydaW - orf, hypothetical protein (VIMSS), at 1,420,725 to 1,421,224
ydaW
b1362: rzpR - putative Rac prophage endopeptidase (VIMSS), at 1,421,225 to 1,421,668
rzpR
b4528: rzoR - Rac prophage; predicted lipoprotein (NCBI), at 1,421,424 to 1,421,609
rzoR
b1363: trkG - Rac prophage; potassium transporter subunit (NCBI), at 1,421,806 to 1,423,263
trkG
b1365: ynaK - Rac prophage; conserved protein (NCBI), at 1,423,401 to 1,423,664
ynaK
b1366: ydaY - Rac prophage; predicted protein (RefSeq), at 1,423,654 to 1,424,106
ydaY
Position (kb)
1421
1422
1423
1424 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1420.831 kb on - strand, within ydaW at 1421.276 kb on - strand, within rzpR at 1421.536 kb on + strand at 1421.536 kb on + strand at 1421.602 kb on + strand at 1421.612 kb on + strand, within rzpR at 1421.612 kb on + strand, within rzpR at 1421.623 kb on + strand, within rzpR at 1421.623 kb on + strand, within rzpR at 1421.638 kb on + strand at 1421.638 kb on + strand at 1421.641 kb on + strand at 1421.672 kb on + strand at 1421.672 kb on + strand at 1421.813 kb on + strand at 1421.838 kb on + strand at 1421.838 kb on + strand at 1421.841 kb on + strand at 1421.841 kb on + strand at 1421.846 kb on + strand at 1421.847 kb on + strand at 1421.848 kb on + strand at 1421.848 kb on + strand at 1421.859 kb on + strand at 1421.875 kb on + strand at 1421.891 kb on + strand at 1421.895 kb on + strand at 1421.983 kb on + strand, within trkG at 1422.251 kb on + strand, within trkG at 1422.251 kb on + strand, within trkG at 1422.308 kb on - strand, within trkG at 1422.353 kb on + strand, within trkG at 1422.475 kb on + strand, within trkG at 1422.846 kb on + strand, within trkG at 1422.986 kb on + strand, within trkG at 1423.139 kb on + strand at 1423.141 kb on + strand at 1423.185 kb on + strand at 1423.263 kb on + strand at 1423.330 kb on - strand at 1423.353 kb on + strand at 1423.358 kb on - strand at 1423.441 kb on + strand, within ynaK at 1423.470 kb on - strand, within ynaK at 1423.470 kb on - strand, within ynaK at 1423.472 kb on + strand, within ynaK at 1423.472 kb on + strand, within ynaK at 1423.477 kb on + strand, within ynaK at 1423.477 kb on + strand, within ynaK at 1423.477 kb on + strand, within ynaK at 1423.477 kb on + strand, within ynaK at 1423.477 kb on + strand, within ynaK at 1423.477 kb on + strand, within ynaK at 1423.480 kb on - strand, within ynaK at 1423.480 kb on - strand, within ynaK at 1423.480 kb on - strand, within ynaK at 1423.480 kb on - strand, within ynaK at 1423.480 kb on - strand, within ynaK at 1423.480 kb on - strand, within ynaK at 1423.482 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.485 kb on - strand, within ynaK at 1423.490 kb on - strand, within ynaK at 1423.507 kb on + strand, within ynaK at 1423.507 kb on - strand, within ynaK at 1423.539 kb on - strand, within ynaK at 1423.577 kb on - strand, within ynaK at 1423.584 kb on - strand, within ynaK at 1423.632 kb on - strand, within ynaK at 1423.632 kb on - strand, within ynaK at 1423.661 kb on + strand at 1423.664 kb on - strand at 1423.902 kb on + strand, within ydaY at 1423.902 kb on + strand, within ydaY at 1423.902 kb on + strand, within ydaY at 1423.937 kb on + strand, within ydaY at 1423.957 kb on + strand, within ydaY at 1423.962 kb on + strand, within ydaY at 1423.962 kb on + strand, within ydaY at 1423.962 kb on + strand, within ydaY at 1424.115 kb on + strand at 1424.126 kb on + strand at 1424.126 kb on + strand at 1424.159 kb on + strand at 1424.197 kb on + strand at 1424.234 kb on + strand at 1424.262 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time1-2hr remove 1,420,831 - ydaW b1361 0.21 +1.2 1,421,276 - rzpR b1362 0.11 +0.4 1,421,536 + -0.1 1,421,536 + +0.3 1,421,602 + -1.5 1,421,612 + rzpR b1362 0.87 +0.3 1,421,612 + rzpR b1362 0.87 -0.6 1,421,623 + rzpR b1362 0.90 +0.0 1,421,623 + rzpR b1362 0.90 -1.1 1,421,638 + +0.6 1,421,638 + -0.2 1,421,641 + -0.2 1,421,672 + +0.9 1,421,672 + +0.6 1,421,813 + -0.1 1,421,838 + +0.1 1,421,838 + +1.0 1,421,841 + -0.9 1,421,841 + -0.6 1,421,846 + -0.5 1,421,847 + -0.4 1,421,848 + +0.2 1,421,848 + -0.1 1,421,859 + -1.7 1,421,875 + -0.1 1,421,891 + +0.1 1,421,895 + +0.1 1,421,983 + trkG b1363 0.12 +0.9 1,422,251 + trkG b1363 0.31 +0.9 1,422,251 + trkG b1363 0.31 +0.9 1,422,308 - trkG b1363 0.34 +0.9 1,422,353 + trkG b1363 0.38 +0.9 1,422,475 + trkG b1363 0.46 +2.5 1,422,846 + trkG b1363 0.71 +0.9 1,422,986 + trkG b1363 0.81 +1.9 1,423,139 + +1.9 1,423,141 + -0.7 1,423,185 + +1.5 1,423,263 + +0.9 1,423,330 - +0.9 1,423,353 + +0.9 1,423,358 - +0.9 1,423,441 + ynaK b1365 0.15 +0.9 1,423,470 - ynaK b1365 0.26 -0.4 1,423,470 - ynaK b1365 0.26 -0.3 1,423,472 + ynaK b1365 0.27 -0.4 1,423,472 + ynaK b1365 0.27 +0.9 1,423,477 + ynaK b1365 0.29 -1.9 1,423,477 + ynaK b1365 0.29 -0.5 1,423,477 + ynaK b1365 0.29 -1.7 1,423,477 + ynaK b1365 0.29 +1.7 1,423,477 + ynaK b1365 0.29 +0.1 1,423,477 + ynaK b1365 0.29 +0.2 1,423,480 - ynaK b1365 0.30 -0.4 1,423,480 - ynaK b1365 0.30 +0.1 1,423,480 - ynaK b1365 0.30 +0.9 1,423,480 - ynaK b1365 0.30 -0.9 1,423,480 - ynaK b1365 0.30 -0.5 1,423,480 - ynaK b1365 0.30 +0.2 1,423,482 - ynaK b1365 0.31 +0.0 1,423,485 - ynaK b1365 0.32 -0.4 1,423,485 - ynaK b1365 0.32 +0.1 1,423,485 - ynaK b1365 0.32 -0.4 1,423,485 - ynaK b1365 0.32 -0.4 1,423,485 - ynaK b1365 0.32 +0.2 1,423,485 - ynaK b1365 0.32 -0.1 1,423,485 - ynaK b1365 0.32 -2.8 1,423,485 - ynaK b1365 0.32 -0.0 1,423,485 - ynaK b1365 0.32 +0.1 1,423,485 - ynaK b1365 0.32 +0.3 1,423,485 - ynaK b1365 0.32 +0.5 1,423,485 - ynaK b1365 0.32 +0.4 1,423,485 - ynaK b1365 0.32 +0.2 1,423,485 - ynaK b1365 0.32 -0.9 1,423,485 - ynaK b1365 0.32 +0.2 1,423,485 - ynaK b1365 0.32 +0.9 1,423,490 - ynaK b1365 0.34 +0.7 1,423,507 + ynaK b1365 0.40 -1.9 1,423,507 - ynaK b1365 0.40 -0.2 1,423,539 - ynaK b1365 0.52 +0.1 1,423,577 - ynaK b1365 0.67 +0.5 1,423,584 - ynaK b1365 0.69 +0.3 1,423,632 - ynaK b1365 0.88 -0.6 1,423,632 - ynaK b1365 0.88 +0.9 1,423,661 + +0.2 1,423,664 - +1.5 1,423,902 + ydaY b1366 0.55 -0.3 1,423,902 + ydaY b1366 0.55 -0.0 1,423,902 + ydaY b1366 0.55 +1.3 1,423,937 + ydaY b1366 0.62 -0.2 1,423,957 + ydaY b1366 0.67 +0.5 1,423,962 + ydaY b1366 0.68 -0.1 1,423,962 + ydaY b1366 0.68 -0.6 1,423,962 + ydaY b1366 0.68 -0.3 1,424,115 + -0.7 1,424,126 + +0.3 1,424,126 + +1.1 1,424,159 + +0.1 1,424,197 + +0.3 1,424,234 + -0.2 1,424,262 + -0.3
Or see this region's nucleotide sequence