Experiment: LB + ciprofloxacin Time1-2hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt minE and minD are separated by 3 nucleotides minD and minC are separated by 23 nucleotides minC and ycgJ are separated by 519 nucleotides ycgJ and ycgK are separated by 102 nucleotides
b1174: minE - cell division topological specificity factor MinE (NCBI), at 1,223,502 to 1,223,768
minE
b1175: minD - membrane ATPase of the MinC-MinD-MinE system (NCBI), at 1,223,772 to 1,224,584
minD
b1176: minC - septum formation inhibitor (NCBI), at 1,224,608 to 1,225,303
minC
b1177: ycgJ - hypothetical protein (NCBI), at 1,225,823 to 1,226,191
ycgJ
b1178: ycgK - hypothetical protein (NCBI), at 1,226,294 to 1,226,695
ycgK
Position (kb)
1224
1225
1226 Strain fitness (log2 ratio)
-2
-1
0
1 at 1223.902 kb on - strand, within minD at 1223.936 kb on - strand, within minD at 1223.941 kb on - strand, within minD at 1223.941 kb on - strand, within minD at 1224.022 kb on - strand, within minD at 1224.061 kb on + strand, within minD at 1224.461 kb on - strand, within minD at 1224.461 kb on - strand, within minD at 1224.509 kb on - strand at 1224.534 kb on + strand at 1224.577 kb on - strand at 1224.684 kb on + strand, within minC at 1224.784 kb on + strand, within minC at 1224.854 kb on - strand, within minC at 1225.133 kb on - strand, within minC at 1225.151 kb on - strand, within minC at 1225.151 kb on - strand, within minC at 1225.310 kb on - strand at 1225.310 kb on - strand at 1225.329 kb on + strand at 1225.354 kb on - strand at 1225.380 kb on - strand at 1225.382 kb on + strand at 1225.405 kb on - strand at 1225.606 kb on + strand at 1225.672 kb on - strand at 1225.677 kb on + strand at 1225.677 kb on + strand at 1225.688 kb on + strand at 1225.688 kb on + strand at 1225.692 kb on + strand at 1225.693 kb on - strand at 1225.700 kb on - strand at 1225.700 kb on - strand at 1225.712 kb on + strand at 1225.720 kb on + strand at 1225.720 kb on + strand at 1225.720 kb on + strand at 1225.728 kb on - strand at 1225.734 kb on + strand at 1225.734 kb on + strand at 1225.734 kb on + strand at 1225.742 kb on - strand at 1225.742 kb on - strand at 1225.744 kb on + strand at 1225.776 kb on - strand at 1225.794 kb on - strand at 1225.820 kb on + strand at 1225.865 kb on + strand, within ycgJ at 1225.865 kb on + strand, within ycgJ at 1225.865 kb on + strand, within ycgJ at 1225.893 kb on - strand, within ycgJ at 1225.916 kb on - strand, within ycgJ at 1225.916 kb on - strand, within ycgJ at 1225.951 kb on - strand, within ycgJ at 1226.030 kb on + strand, within ycgJ at 1226.037 kb on + strand, within ycgJ at 1226.037 kb on + strand, within ycgJ at 1226.217 kb on - strand at 1226.219 kb on + strand at 1226.219 kb on + strand at 1226.232 kb on + strand at 1226.234 kb on + strand at 1226.237 kb on + strand at 1226.237 kb on + strand at 1226.240 kb on + strand at 1226.240 kb on + strand at 1226.240 kb on + strand at 1226.240 kb on + strand at 1226.240 kb on - strand at 1226.243 kb on - strand at 1226.243 kb on - strand at 1226.244 kb on + strand at 1226.248 kb on - strand at 1226.276 kb on + strand at 1226.284 kb on - strand at 1226.284 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time1-2hr remove 1,223,902 - minD b1175 0.16 +0.5 1,223,936 - minD b1175 0.20 -0.3 1,223,941 - minD b1175 0.21 +0.1 1,223,941 - minD b1175 0.21 +0.5 1,224,022 - minD b1175 0.31 +0.0 1,224,061 + minD b1175 0.36 +0.8 1,224,461 - minD b1175 0.85 +0.3 1,224,461 - minD b1175 0.85 +0.8 1,224,509 - +0.8 1,224,534 + +0.8 1,224,577 - -0.2 1,224,684 + minC b1176 0.11 -0.2 1,224,784 + minC b1176 0.25 +0.4 1,224,854 - minC b1176 0.35 +0.4 1,225,133 - minC b1176 0.75 -0.4 1,225,151 - minC b1176 0.78 +1.5 1,225,151 - minC b1176 0.78 +0.6 1,225,310 - -0.3 1,225,310 - -0.2 1,225,329 + -0.3 1,225,354 - -0.4 1,225,380 - -0.4 1,225,382 + +0.8 1,225,405 - +0.1 1,225,606 + -0.8 1,225,672 - -0.2 1,225,677 + +0.7 1,225,677 + +0.2 1,225,688 + +0.1 1,225,688 + -0.9 1,225,692 + -0.3 1,225,693 - -0.1 1,225,700 - -0.4 1,225,700 - +0.4 1,225,712 + -0.3 1,225,720 + -0.2 1,225,720 + -0.5 1,225,720 + +0.1 1,225,728 - +0.6 1,225,734 + +0.1 1,225,734 + +0.5 1,225,734 + +0.2 1,225,742 - +0.3 1,225,742 - -0.1 1,225,744 + +0.1 1,225,776 - -1.1 1,225,794 - -0.8 1,225,820 + -0.7 1,225,865 + ycgJ b1177 0.11 -0.0 1,225,865 + ycgJ b1177 0.11 +0.0 1,225,865 + ycgJ b1177 0.11 -0.1 1,225,893 - ycgJ b1177 0.19 +0.8 1,225,916 - ycgJ b1177 0.25 +0.8 1,225,916 - ycgJ b1177 0.25 +0.8 1,225,951 - ycgJ b1177 0.35 +0.8 1,226,030 + ycgJ b1177 0.56 -0.4 1,226,037 + ycgJ b1177 0.58 +0.1 1,226,037 + ycgJ b1177 0.58 +0.6 1,226,217 - +0.8 1,226,219 + -0.1 1,226,219 + +0.3 1,226,232 + +0.3 1,226,234 + -0.5 1,226,237 + -0.3 1,226,237 + -0.1 1,226,240 + +1.5 1,226,240 + -1.0 1,226,240 + -0.2 1,226,240 + -0.7 1,226,240 - +0.8 1,226,243 - +0.8 1,226,243 - +0.0 1,226,244 + +0.3 1,226,248 - +0.8 1,226,276 + -2.0 1,226,284 - +0.0 1,226,284 - +0.4
Or see this region's nucleotide sequence