Experiment: P. putida PUT 7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2507 and PP_5532 are separated by 171 nucleotides PP_5532 and PP_5533 are separated by 149 nucleotides PP_5533 and PP_2508 are separated by 398 nucleotides PP_2508 and PP_5534 overlap by 4 nucleotides PP_5534 and PP_2509 overlap by 4 nucleotides
PP_2507: PP_2507 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 2,854,234 to 2,854,950
_2507
PP_5532: PP_5532 - conserved protein of unknown function, at 2,855,122 to 2,855,385
_5532
PP_5533: PP_5533 - conserved protein of unknown function, at 2,855,535 to 2,855,777
_5533
PP_2508: PP_2508 - conserved protein of unknown function, at 2,856,176 to 2,856,343
_2508
PP_5534: PP_5534 - protein of unknown function, at 2,856,340 to 2,856,570
_5534
PP_2509: PP_2509 - conserved protein of unknown function, at 2,856,567 to 2,856,731
_2509
Position (kb)
2855
2856 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2854.774 kb on + strand, within PP_2507 at 2854.908 kb on - strand at 2855.051 kb on + strand at 2855.087 kb on + strand at 2855.087 kb on + strand at 2855.087 kb on + strand at 2855.088 kb on - strand at 2855.088 kb on - strand at 2855.088 kb on - strand at 2855.088 kb on - strand at 2855.180 kb on + strand, within PP_5532 at 2855.181 kb on - strand, within PP_5532 at 2855.246 kb on + strand, within PP_5532 at 2855.247 kb on - strand, within PP_5532 at 2855.290 kb on + strand, within PP_5532 at 2855.290 kb on + strand, within PP_5532 at 2855.290 kb on + strand, within PP_5532 at 2855.290 kb on + strand, within PP_5532 at 2855.290 kb on + strand, within PP_5532 at 2855.291 kb on - strand, within PP_5532 at 2855.339 kb on + strand, within PP_5532 at 2855.339 kb on + strand at 2855.340 kb on - strand, within PP_5532 at 2855.340 kb on - strand, within PP_5532 at 2855.340 kb on - strand, within PP_5532 at 2855.340 kb on - strand, within PP_5532 at 2855.340 kb on - strand, within PP_5532 at 2855.520 kb on + strand at 2855.520 kb on + strand at 2855.520 kb on + strand at 2855.543 kb on + strand at 2855.578 kb on - strand, within PP_5533 at 2855.783 kb on - strand at 2855.807 kb on + strand at 2855.808 kb on - strand at 2855.808 kb on - strand at 2855.808 kb on - strand at 2855.845 kb on + strand at 2855.903 kb on - strand at 2856.173 kb on + strand at 2856.173 kb on + strand at 2856.173 kb on + strand at 2856.174 kb on - strand at 2856.174 kb on - strand at 2856.422 kb on + strand, within PP_5534 at 2856.422 kb on + strand, within PP_5534 at 2856.422 kb on + strand, within PP_5534 at 2856.423 kb on - strand, within PP_5534 at 2856.423 kb on - strand, within PP_5534 at 2856.423 kb on - strand, within PP_5534 at 2856.423 kb on - strand, within PP_5534 at 2856.423 kb on - strand, within PP_5534 at 2856.423 kb on - strand, within PP_5534 at 2856.470 kb on - strand, within PP_5534 at 2856.470 kb on - strand, within PP_5534 at 2856.470 kb on - strand, within PP_5534 at 2856.569 kb on - strand at 2856.576 kb on + strand at 2856.577 kb on - strand at 2856.577 kb on - strand at 2856.653 kb on + strand, within PP_2509 at 2856.654 kb on - strand, within PP_2509 at 2856.654 kb on - strand, within PP_2509 at 2856.658 kb on - strand, within PP_2509 at 2856.742 kb on - strand at 2856.742 kb on - strand at 2856.742 kb on - strand at 2856.770 kb on + strand at 2856.771 kb on - strand at 2856.771 kb on - strand at 2856.775 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT 7 MOI 1 remove 2,854,774 + PP_2507 0.75 -2.8 2,854,908 - +1.4 2,855,051 + -1.3 2,855,087 + -1.5 2,855,087 + +1.4 2,855,087 + +0.8 2,855,088 - -2.0 2,855,088 - +1.7 2,855,088 - -0.8 2,855,088 - +0.2 2,855,180 + PP_5532 0.22 +1.6 2,855,181 - PP_5532 0.22 +0.6 2,855,246 + PP_5532 0.47 -0.2 2,855,247 - PP_5532 0.47 -0.3 2,855,290 + PP_5532 0.64 -0.2 2,855,290 + PP_5532 0.64 +1.1 2,855,290 + PP_5532 0.64 +0.8 2,855,290 + PP_5532 0.64 +1.7 2,855,290 + PP_5532 0.64 -3.0 2,855,291 - PP_5532 0.64 -1.0 2,855,339 + PP_5532 0.82 +0.7 2,855,339 + +1.6 2,855,340 - PP_5532 0.83 -2.0 2,855,340 - PP_5532 0.83 -0.3 2,855,340 - PP_5532 0.83 -2.2 2,855,340 - PP_5532 0.83 -1.5 2,855,340 - PP_5532 0.83 +1.4 2,855,520 + -1.7 2,855,520 + +0.4 2,855,520 + +0.9 2,855,543 + -0.9 2,855,578 - PP_5533 0.18 -1.6 2,855,783 - -2.6 2,855,807 + -1.3 2,855,808 - +1.4 2,855,808 - -1.7 2,855,808 - -2.4 2,855,845 + -2.4 2,855,903 - +0.7 2,856,173 + -1.7 2,856,173 + +0.3 2,856,173 + -2.4 2,856,174 - -2.1 2,856,174 - +1.4 2,856,422 + PP_5534 0.35 +1.9 2,856,422 + PP_5534 0.35 +1.3 2,856,422 + PP_5534 0.35 -1.0 2,856,423 - PP_5534 0.36 -2.1 2,856,423 - PP_5534 0.36 +1.3 2,856,423 - PP_5534 0.36 +0.7 2,856,423 - PP_5534 0.36 +0.4 2,856,423 - PP_5534 0.36 -0.1 2,856,423 - PP_5534 0.36 +0.8 2,856,470 - PP_5534 0.56 +0.6 2,856,470 - PP_5534 0.56 -0.9 2,856,470 - PP_5534 0.56 +1.6 2,856,569 - +1.0 2,856,576 + -0.7 2,856,577 - -0.9 2,856,577 - +0.2 2,856,653 + PP_2509 0.52 -0.6 2,856,654 - PP_2509 0.53 +0.4 2,856,654 - PP_2509 0.53 +1.7 2,856,658 - PP_2509 0.55 +0.7 2,856,742 - -2.0 2,856,742 - +1.0 2,856,742 - +0.7 2,856,770 + -2.6 2,856,771 - -1.8 2,856,771 - -0.2 2,856,775 - +0.2
Or see this region's nucleotide sequence