Experiment: P. putida PUT 7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3274 and PP_3275 are separated by 15 nucleotides PP_3275 and PP_3276 overlap by 14 nucleotides PP_3276 and PP_3277 are separated by 8 nucleotides PP_3277 and PP_3278 are separated by 25 nucleotides
PP_3274: PP_3274 - ring 1,2-phenylacetyl-CoA epoxidase, reductase subunit, at 3,709,320 to 3,710,396
_3274
PP_3275: PP_3275 - putative subunit of phenylacetate degradation enzyme, at 3,710,412 to 3,710,945
_3275
PP_3276: PP_3276 - ring 1,2-phenylacetyl-CoA epoxidase beta subunit, at 3,710,932 to 3,711,690
_3276
PP_3277: PP_3277 - ring 1,2-phenylacetyl-CoA epoxidase regulatory subunit, at 3,711,699 to 3,711,980
_3277
PP_3278: PP_3278 - ring 1,2-phenylacetyl-CoA epoxidase alpha subunit, at 3,712,006 to 3,712,995
_3278
Position (kb)
3710
3711
3712 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3709.958 kb on - strand, within PP_3274 at 3709.958 kb on - strand, within PP_3274 at 3709.997 kb on + strand, within PP_3274 at 3709.998 kb on - strand, within PP_3274 at 3710.017 kb on - strand, within PP_3274 at 3710.043 kb on + strand, within PP_3274 at 3710.053 kb on + strand, within PP_3274 at 3710.054 kb on - strand, within PP_3274 at 3710.054 kb on - strand, within PP_3274 at 3710.093 kb on + strand, within PP_3274 at 3710.227 kb on + strand, within PP_3274 at 3710.262 kb on - strand, within PP_3274 at 3710.275 kb on + strand, within PP_3274 at 3710.276 kb on - strand, within PP_3274 at 3710.276 kb on - strand, within PP_3274 at 3710.462 kb on - strand at 3710.753 kb on - strand, within PP_3275 at 3710.753 kb on - strand, within PP_3275 at 3710.753 kb on - strand, within PP_3275 at 3711.029 kb on + strand, within PP_3276 at 3711.029 kb on + strand, within PP_3276 at 3711.287 kb on + strand, within PP_3276 at 3711.287 kb on + strand, within PP_3276 at 3711.288 kb on - strand, within PP_3276 at 3711.522 kb on - strand, within PP_3276 at 3711.522 kb on - strand, within PP_3276 at 3711.665 kb on + strand at 3711.665 kb on + strand at 3711.684 kb on + strand at 3711.684 kb on + strand at 3711.684 kb on + strand at 3711.684 kb on + strand at 3711.685 kb on - strand at 3711.685 kb on - strand at 3711.685 kb on - strand at 3711.731 kb on - strand, within PP_3277 at 3711.748 kb on + strand, within PP_3277 at 3711.749 kb on - strand, within PP_3277 at 3711.749 kb on - strand, within PP_3277 at 3711.849 kb on - strand, within PP_3277 at 3711.860 kb on - strand, within PP_3277 at 3711.860 kb on - strand, within PP_3277 at 3712.040 kb on + strand at 3712.040 kb on + strand at 3712.041 kb on - strand at 3712.142 kb on + strand, within PP_3278 at 3712.176 kb on - strand, within PP_3278 at 3712.489 kb on - strand, within PP_3278 at 3712.534 kb on + strand, within PP_3278 at 3712.535 kb on - strand, within PP_3278 at 3712.610 kb on + strand, within PP_3278 at 3712.610 kb on + strand, within PP_3278 at 3712.611 kb on - strand, within PP_3278 at 3712.628 kb on + strand, within PP_3278
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT 7 MOI 1 remove 3,709,958 - PP_3274 0.59 -1.6 3,709,958 - PP_3274 0.59 -2.3 3,709,997 + PP_3274 0.63 -0.6 3,709,998 - PP_3274 0.63 -0.3 3,710,017 - PP_3274 0.65 +1.2 3,710,043 + PP_3274 0.67 -0.5 3,710,053 + PP_3274 0.68 +1.0 3,710,054 - PP_3274 0.68 +0.0 3,710,054 - PP_3274 0.68 -1.6 3,710,093 + PP_3274 0.72 +1.5 3,710,227 + PP_3274 0.84 -2.9 3,710,262 - PP_3274 0.87 +3.1 3,710,275 + PP_3274 0.89 +1.7 3,710,276 - PP_3274 0.89 -0.0 3,710,276 - PP_3274 0.89 +0.7 3,710,462 - -0.6 3,710,753 - PP_3275 0.64 +1.9 3,710,753 - PP_3275 0.64 -1.1 3,710,753 - PP_3275 0.64 -0.7 3,711,029 + PP_3276 0.13 -3.4 3,711,029 + PP_3276 0.13 -0.7 3,711,287 + PP_3276 0.47 -2.8 3,711,287 + PP_3276 0.47 -2.6 3,711,288 - PP_3276 0.47 -1.9 3,711,522 - PP_3276 0.78 +0.9 3,711,522 - PP_3276 0.78 -1.6 3,711,665 + -2.7 3,711,665 + +1.8 3,711,684 + -2.7 3,711,684 + -1.3 3,711,684 + -3.0 3,711,684 + +0.9 3,711,685 - +0.7 3,711,685 - -0.5 3,711,685 - -0.6 3,711,731 - PP_3277 0.11 -0.5 3,711,748 + PP_3277 0.17 -1.9 3,711,749 - PP_3277 0.18 +2.0 3,711,749 - PP_3277 0.18 -1.4 3,711,849 - PP_3277 0.53 +2.1 3,711,860 - PP_3277 0.57 +0.1 3,711,860 - PP_3277 0.57 -2.4 3,712,040 + -1.8 3,712,040 + -0.8 3,712,041 - +0.3 3,712,142 + PP_3278 0.14 +0.3 3,712,176 - PP_3278 0.17 +0.2 3,712,489 - PP_3278 0.49 -2.6 3,712,534 + PP_3278 0.53 -1.2 3,712,535 - PP_3278 0.53 -2.2 3,712,610 + PP_3278 0.61 +0.8 3,712,610 + PP_3278 0.61 -0.4 3,712,611 - PP_3278 0.61 +0.2 3,712,628 + PP_3278 0.63 +2.0
Or see this region's nucleotide sequence