Experiment: P. putida PUT 7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3253 and PP_3254 overlap by 10 nucleotides PP_3254 and PP_3255 are separated by 85 nucleotides PP_3255 and PP_3256 are separated by 21 nucleotides
PP_3253: PP_3253 - Carboxylate-amine ligase PP_3253, at 3,687,884 to 3,688,990
_3253
PP_3254: PP_3254 - putative Nucleosidase, at 3,688,981 to 3,689,634
_3254
PP_3255: PP_3255 - non-homologous end joining protein, at 3,689,720 to 3,690,541
_3255
PP_3256: PP_3256 - Glycosyl transferase, group 2 family protein, at 3,690,563 to 3,691,222
_3256
Position (kb)
3688
3689
3690 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3688.008 kb on - strand, within PP_3253 at 3688.070 kb on + strand, within PP_3253 at 3688.145 kb on + strand, within PP_3253 at 3688.145 kb on + strand, within PP_3253 at 3688.208 kb on + strand, within PP_3253 at 3688.208 kb on + strand, within PP_3253 at 3688.209 kb on - strand, within PP_3253 at 3688.209 kb on - strand, within PP_3253 at 3688.209 kb on - strand, within PP_3253 at 3688.210 kb on + strand, within PP_3253 at 3688.210 kb on + strand, within PP_3253 at 3688.211 kb on - strand, within PP_3253 at 3688.211 kb on - strand, within PP_3253 at 3688.250 kb on - strand, within PP_3253 at 3688.250 kb on - strand, within PP_3253 at 3688.344 kb on + strand, within PP_3253 at 3688.387 kb on + strand, within PP_3253 at 3688.388 kb on - strand, within PP_3253 at 3688.472 kb on + strand, within PP_3253 at 3688.718 kb on + strand, within PP_3253 at 3688.718 kb on + strand, within PP_3253 at 3688.718 kb on + strand, within PP_3253 at 3688.719 kb on - strand, within PP_3253 at 3688.758 kb on + strand, within PP_3253 at 3688.759 kb on - strand, within PP_3253 at 3688.821 kb on + strand, within PP_3253 at 3688.821 kb on + strand, within PP_3253 at 3688.821 kb on + strand, within PP_3253 at 3688.827 kb on + strand at 3688.827 kb on + strand, within PP_3253 at 3688.827 kb on + strand, within PP_3253 at 3688.885 kb on + strand at 3688.885 kb on + strand at 3689.055 kb on + strand, within PP_3254 at 3689.056 kb on - strand, within PP_3254 at 3689.056 kb on - strand, within PP_3254 at 3689.479 kb on + strand, within PP_3254 at 3689.479 kb on + strand, within PP_3254 at 3689.529 kb on + strand, within PP_3254 at 3689.529 kb on + strand, within PP_3254 at 3689.529 kb on + strand, within PP_3254 at 3689.530 kb on - strand, within PP_3254 at 3689.530 kb on - strand, within PP_3254 at 3689.530 kb on - strand, within PP_3254 at 3689.530 kb on - strand at 3689.533 kb on + strand, within PP_3254 at 3689.534 kb on - strand, within PP_3254 at 3689.579 kb on + strand at 3689.691 kb on + strand at 3689.899 kb on + strand, within PP_3255 at 3689.900 kb on - strand, within PP_3255 at 3689.933 kb on + strand, within PP_3255 at 3689.933 kb on + strand, within PP_3255 at 3689.934 kb on - strand, within PP_3255 at 3689.934 kb on - strand, within PP_3255 at 3690.051 kb on - strand, within PP_3255 at 3690.051 kb on - strand, within PP_3255 at 3690.054 kb on - strand, within PP_3255 at 3690.086 kb on + strand, within PP_3255 at 3690.086 kb on + strand, within PP_3255 at 3690.087 kb on - strand at 3690.087 kb on - strand, within PP_3255 at 3690.087 kb on - strand, within PP_3255 at 3690.144 kb on + strand, within PP_3255 at 3690.144 kb on + strand, within PP_3255 at 3690.145 kb on - strand, within PP_3255 at 3690.145 kb on - strand, within PP_3255 at 3690.145 kb on - strand, within PP_3255 at 3690.316 kb on - strand, within PP_3255 at 3690.316 kb on - strand, within PP_3255 at 3690.350 kb on + strand, within PP_3255 at 3690.350 kb on + strand, within PP_3255 at 3690.350 kb on + strand, within PP_3255 at 3690.351 kb on - strand, within PP_3255
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT 7 MOI 1 remove 3,688,008 - PP_3253 0.11 +1.2 3,688,070 + PP_3253 0.17 -3.0 3,688,145 + PP_3253 0.24 -1.9 3,688,145 + PP_3253 0.24 +1.4 3,688,208 + PP_3253 0.29 -0.3 3,688,208 + PP_3253 0.29 +0.9 3,688,209 - PP_3253 0.29 -2.3 3,688,209 - PP_3253 0.29 -1.1 3,688,209 - PP_3253 0.29 -0.7 3,688,210 + PP_3253 0.29 +0.1 3,688,210 + PP_3253 0.29 +1.9 3,688,211 - PP_3253 0.30 +1.1 3,688,211 - PP_3253 0.30 +0.2 3,688,250 - PP_3253 0.33 -0.4 3,688,250 - PP_3253 0.33 -2.0 3,688,344 + PP_3253 0.42 -0.9 3,688,387 + PP_3253 0.45 +2.5 3,688,388 - PP_3253 0.46 -2.5 3,688,472 + PP_3253 0.53 +0.9 3,688,718 + PP_3253 0.75 +0.7 3,688,718 + PP_3253 0.75 +1.3 3,688,718 + PP_3253 0.75 -0.3 3,688,719 - PP_3253 0.75 -0.7 3,688,758 + PP_3253 0.79 -1.8 3,688,759 - PP_3253 0.79 -2.3 3,688,821 + PP_3253 0.85 +0.7 3,688,821 + PP_3253 0.85 -0.2 3,688,821 + PP_3253 0.85 +1.0 3,688,827 + +1.9 3,688,827 + PP_3253 0.85 +0.2 3,688,827 + PP_3253 0.85 +0.3 3,688,885 + -0.1 3,688,885 + -1.3 3,689,055 + PP_3254 0.11 +0.7 3,689,056 - PP_3254 0.11 -1.7 3,689,056 - PP_3254 0.11 -1.6 3,689,479 + PP_3254 0.76 -2.6 3,689,479 + PP_3254 0.76 +0.3 3,689,529 + PP_3254 0.84 +1.9 3,689,529 + PP_3254 0.84 -2.5 3,689,529 + PP_3254 0.84 +0.1 3,689,530 - PP_3254 0.84 -2.6 3,689,530 - PP_3254 0.84 +0.6 3,689,530 - PP_3254 0.84 -2.5 3,689,530 - +1.9 3,689,533 + PP_3254 0.84 -2.4 3,689,534 - PP_3254 0.85 +0.1 3,689,579 + -2.3 3,689,691 + -1.3 3,689,899 + PP_3255 0.22 -1.4 3,689,900 - PP_3255 0.22 -0.7 3,689,933 + PP_3255 0.26 -0.3 3,689,933 + PP_3255 0.26 -0.6 3,689,934 - PP_3255 0.26 -2.9 3,689,934 - PP_3255 0.26 -1.0 3,690,051 - PP_3255 0.40 -2.6 3,690,051 - PP_3255 0.40 +1.9 3,690,054 - PP_3255 0.41 -1.2 3,690,086 + PP_3255 0.45 -0.6 3,690,086 + PP_3255 0.45 +0.1 3,690,087 - +1.1 3,690,087 - PP_3255 0.45 +0.1 3,690,087 - PP_3255 0.45 -0.3 3,690,144 + PP_3255 0.52 -1.3 3,690,144 + PP_3255 0.52 +0.2 3,690,145 - PP_3255 0.52 +0.2 3,690,145 - PP_3255 0.52 -1.1 3,690,145 - PP_3255 0.52 +0.7 3,690,316 - PP_3255 0.73 -0.2 3,690,316 - PP_3255 0.73 -0.4 3,690,350 + PP_3255 0.77 -2.9 3,690,350 + PP_3255 0.77 -1.9 3,690,350 + PP_3255 0.77 +0.4 3,690,351 - PP_3255 0.77 +0.9
Or see this region's nucleotide sequence