Experiment: P. putida PUT 7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3047 and PP_3048 overlap by 4 nucleotides PP_3048 and PP_3049 overlap by 4 nucleotides PP_3049 and PP_3050 overlap by 8 nucleotides PP_3050 and PP_3051 overlap by 4 nucleotides PP_3051 and PP_3052 are separated by 42 nucleotides PP_3052 and PP_3053 are separated by 2 nucleotides
PP_3047: PP_3047 - conserved protein of unknown function, at 3,431,358 to 3,432,353
_3047
PP_3048: PP_3048 - conserved protein of unknown function, at 3,432,350 to 3,432,670
_3048
PP_3049: PP_3049 - conserved protein of unknown function, at 3,432,667 to 3,433,329
_3049
PP_3050: PP_3050 - conserved protein of unknown function, at 3,433,322 to 3,433,852
_3050
PP_3051: PP_3051 - Pyocin R2_PP, tail spike protein, at 3,433,849 to 3,434,451
_3051
PP_3052: PP_3052 - conserved protein of unknown function, at 3,434,494 to 3,434,763
_3052
PP_3053: PP_3053 - Pyocin R2_PP, baseplate protein, at 3,434,766 to 3,435,092
_3053
Position (kb)
3433
3434 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3432.602 kb on + strand, within PP_3048 at 3432.603 kb on - strand at 3432.603 kb on - strand, within PP_3048 at 3432.603 kb on - strand, within PP_3048 at 3432.642 kb on + strand at 3432.643 kb on - strand at 3432.643 kb on - strand at 3432.666 kb on + strand at 3432.667 kb on - strand at 3432.679 kb on - strand at 3432.679 kb on - strand at 3432.679 kb on - strand at 3432.682 kb on + strand at 3432.682 kb on + strand at 3432.852 kb on - strand, within PP_3049 at 3432.852 kb on - strand, within PP_3049 at 3432.852 kb on - strand, within PP_3049 at 3432.873 kb on + strand, within PP_3049 at 3432.873 kb on + strand, within PP_3049 at 3432.873 kb on + strand, within PP_3049 at 3433.021 kb on + strand, within PP_3049 at 3433.048 kb on + strand, within PP_3049 at 3433.077 kb on + strand, within PP_3049 at 3433.125 kb on - strand, within PP_3049 at 3433.262 kb on + strand, within PP_3049 at 3433.262 kb on + strand, within PP_3049 at 3433.262 kb on + strand, within PP_3049 at 3433.303 kb on + strand at 3433.355 kb on + strand at 3433.403 kb on + strand, within PP_3050 at 3433.404 kb on - strand, within PP_3050 at 3433.562 kb on - strand, within PP_3050 at 3433.562 kb on - strand, within PP_3050 at 3433.575 kb on + strand, within PP_3050 at 3433.575 kb on + strand, within PP_3050 at 3433.575 kb on + strand, within PP_3050 at 3433.575 kb on + strand, within PP_3050 at 3433.575 kb on + strand, within PP_3050 at 3433.575 kb on + strand, within PP_3050 at 3433.576 kb on - strand, within PP_3050 at 3433.576 kb on - strand, within PP_3050 at 3433.576 kb on - strand, within PP_3050 at 3433.576 kb on - strand, within PP_3050 at 3433.576 kb on - strand, within PP_3050 at 3433.826 kb on + strand at 3433.827 kb on - strand at 3433.827 kb on - strand at 3433.827 kb on - strand at 3433.906 kb on + strand at 3433.906 kb on + strand at 3433.907 kb on - strand at 3434.179 kb on + strand, within PP_3051 at 3434.196 kb on + strand, within PP_3051 at 3434.197 kb on - strand, within PP_3051 at 3434.200 kb on + strand, within PP_3051 at 3434.201 kb on - strand, within PP_3051 at 3434.201 kb on - strand, within PP_3051 at 3434.382 kb on - strand, within PP_3051 at 3434.494 kb on - strand at 3434.617 kb on + strand, within PP_3052 at 3434.799 kb on - strand, within PP_3053 at 3434.799 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT 7 MOI 1 remove 3,432,602 + PP_3048 0.79 -3.3 3,432,603 - +1.5 3,432,603 - PP_3048 0.79 +0.3 3,432,603 - PP_3048 0.79 +0.2 3,432,642 + -0.6 3,432,643 - -0.2 3,432,643 - -2.8 3,432,666 + -1.5 3,432,667 - -2.0 3,432,679 - +1.5 3,432,679 - -1.4 3,432,679 - -3.5 3,432,682 + -3.0 3,432,682 + -2.6 3,432,852 - PP_3049 0.28 -1.9 3,432,852 - PP_3049 0.28 -0.8 3,432,852 - PP_3049 0.28 -0.7 3,432,873 + PP_3049 0.31 -0.5 3,432,873 + PP_3049 0.31 -0.5 3,432,873 + PP_3049 0.31 -0.6 3,433,021 + PP_3049 0.53 +0.4 3,433,048 + PP_3049 0.57 +3.3 3,433,077 + PP_3049 0.62 -1.9 3,433,125 - PP_3049 0.69 +2.7 3,433,262 + PP_3049 0.90 -2.8 3,433,262 + PP_3049 0.90 -0.3 3,433,262 + PP_3049 0.90 -3.0 3,433,303 + -0.0 3,433,355 + -1.8 3,433,403 + PP_3050 0.15 -1.8 3,433,404 - PP_3050 0.15 -0.3 3,433,562 - PP_3050 0.45 +0.5 3,433,562 - PP_3050 0.45 -0.5 3,433,575 + PP_3050 0.48 +0.3 3,433,575 + PP_3050 0.48 -3.0 3,433,575 + PP_3050 0.48 +0.5 3,433,575 + PP_3050 0.48 -3.2 3,433,575 + PP_3050 0.48 -0.8 3,433,575 + PP_3050 0.48 -2.8 3,433,576 - PP_3050 0.48 -0.5 3,433,576 - PP_3050 0.48 -0.5 3,433,576 - PP_3050 0.48 -1.9 3,433,576 - PP_3050 0.48 +0.8 3,433,576 - PP_3050 0.48 -0.9 3,433,826 + -0.0 3,433,827 - +0.8 3,433,827 - -3.5 3,433,827 - +0.1 3,433,906 + -2.5 3,433,906 + -0.8 3,433,907 - +1.3 3,434,179 + PP_3051 0.55 +0.7 3,434,196 + PP_3051 0.58 -0.8 3,434,197 - PP_3051 0.58 -0.1 3,434,200 + PP_3051 0.58 -2.8 3,434,201 - PP_3051 0.58 -0.7 3,434,201 - PP_3051 0.58 -0.7 3,434,382 - PP_3051 0.88 -1.5 3,434,494 - +0.7 3,434,617 + PP_3052 0.46 +1.0 3,434,799 - PP_3053 0.10 -0.5 3,434,799 - +1.0
Or see this region's nucleotide sequence