Experiment: P. putida PUT 7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2513 and PP_2514 are separated by 2 nucleotides PP_2514 and PP_2515 overlap by 4 nucleotides PP_2515 and PP_2516 are separated by 117 nucleotides
PP_2513: PP_2513 - 4-oxalomesaconate tautomerase, at 2,860,246 to 2,861,331
_2513
PP_2514: PP_2514 - 4-carboxy-4-hydroxy-2-oxoadipic acid aldolase, at 2,861,334 to 2,862,050
_2514
PP_2515: PP_2515 - 4-oxalmesaconate hydratase, at 2,862,047 to 2,862,823
_2515
PP_2516: PP_2516 - HTH-type transcriptional regulator GalR, at 2,862,941 to 2,864,134
_2516
Position (kb)
2862
2863 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2861.060 kb on + strand, within PP_2513 at 2861.061 kb on - strand, within PP_2513 at 2861.102 kb on + strand, within PP_2513 at 2861.102 kb on + strand, within PP_2513 at 2861.314 kb on + strand at 2861.365 kb on + strand at 2861.366 kb on - strand at 2861.404 kb on + strand at 2861.404 kb on + strand at 2861.404 kb on + strand at 2861.405 kb on - strand at 2861.405 kb on - strand at 2861.618 kb on + strand, within PP_2514 at 2861.619 kb on - strand, within PP_2514 at 2861.619 kb on - strand, within PP_2514 at 2861.793 kb on + strand, within PP_2514 at 2861.794 kb on - strand, within PP_2514 at 2861.794 kb on - strand, within PP_2514 at 2861.916 kb on - strand, within PP_2514 at 2862.008 kb on + strand at 2862.008 kb on + strand at 2862.011 kb on + strand at 2862.011 kb on + strand at 2862.012 kb on - strand at 2862.012 kb on - strand at 2862.012 kb on - strand at 2862.012 kb on - strand at 2862.134 kb on - strand, within PP_2515 at 2862.165 kb on - strand, within PP_2515 at 2862.174 kb on + strand, within PP_2515 at 2862.174 kb on + strand, within PP_2515 at 2862.174 kb on + strand, within PP_2515 at 2862.174 kb on + strand, within PP_2515 at 2862.174 kb on + strand, within PP_2515 at 2862.175 kb on - strand, within PP_2515 at 2862.175 kb on - strand, within PP_2515 at 2862.175 kb on - strand, within PP_2515 at 2862.177 kb on + strand, within PP_2515 at 2862.177 kb on + strand, within PP_2515 at 2862.178 kb on - strand, within PP_2515 at 2862.178 kb on - strand, within PP_2515 at 2862.222 kb on + strand, within PP_2515 at 2862.223 kb on - strand, within PP_2515 at 2862.244 kb on + strand, within PP_2515 at 2862.244 kb on + strand, within PP_2515 at 2862.245 kb on - strand, within PP_2515 at 2862.306 kb on + strand, within PP_2515 at 2862.346 kb on - strand, within PP_2515 at 2862.364 kb on + strand, within PP_2515 at 2862.409 kb on - strand, within PP_2515 at 2862.409 kb on - strand, within PP_2515 at 2862.414 kb on + strand, within PP_2515 at 2862.414 kb on + strand, within PP_2515 at 2862.414 kb on + strand, within PP_2515 at 2862.414 kb on + strand, within PP_2515 at 2862.440 kb on + strand, within PP_2515 at 2862.441 kb on - strand, within PP_2515 at 2862.513 kb on + strand, within PP_2515 at 2862.682 kb on - strand, within PP_2515 at 2862.876 kb on + strand at 2862.895 kb on + strand at 2862.904 kb on - strand at 2862.940 kb on + strand at 2863.229 kb on + strand, within PP_2516 at 2863.230 kb on - strand, within PP_2516 at 2863.566 kb on + strand, within PP_2516 at 2863.567 kb on - strand, within PP_2516 at 2863.567 kb on - strand, within PP_2516 at 2863.691 kb on + strand, within PP_2516
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT 7 MOI 1 remove 2,861,060 + PP_2513 0.75 -0.7 2,861,061 - PP_2513 0.75 +1.8 2,861,102 + PP_2513 0.79 -1.0 2,861,102 + PP_2513 0.79 -0.9 2,861,314 + +1.6 2,861,365 + -0.0 2,861,366 - +1.7 2,861,404 + +0.5 2,861,404 + -1.6 2,861,404 + +1.6 2,861,405 - -2.1 2,861,405 - -0.3 2,861,618 + PP_2514 0.40 +4.2 2,861,619 - PP_2514 0.40 +1.2 2,861,619 - PP_2514 0.40 -1.7 2,861,793 + PP_2514 0.64 +0.6 2,861,794 - PP_2514 0.64 -0.4 2,861,794 - PP_2514 0.64 -2.4 2,861,916 - PP_2514 0.81 +0.8 2,862,008 + -0.6 2,862,008 + +2.7 2,862,011 + +1.6 2,862,011 + -0.7 2,862,012 - -2.2 2,862,012 - -1.3 2,862,012 - -0.1 2,862,012 - +0.8 2,862,134 - PP_2515 0.11 +2.2 2,862,165 - PP_2515 0.15 +1.8 2,862,174 + PP_2515 0.16 -1.6 2,862,174 + PP_2515 0.16 -2.0 2,862,174 + PP_2515 0.16 +0.9 2,862,174 + PP_2515 0.16 -0.4 2,862,174 + PP_2515 0.16 -0.0 2,862,175 - PP_2515 0.16 +0.8 2,862,175 - PP_2515 0.16 +0.6 2,862,175 - PP_2515 0.16 +1.3 2,862,177 + PP_2515 0.17 +0.9 2,862,177 + PP_2515 0.17 -2.7 2,862,178 - PP_2515 0.17 +1.2 2,862,178 - PP_2515 0.17 -2.8 2,862,222 + PP_2515 0.23 +0.6 2,862,223 - PP_2515 0.23 -1.3 2,862,244 + PP_2515 0.25 -3.2 2,862,244 + PP_2515 0.25 -2.6 2,862,245 - PP_2515 0.25 -1.2 2,862,306 + PP_2515 0.33 -0.5 2,862,346 - PP_2515 0.38 -0.4 2,862,364 + PP_2515 0.41 +1.9 2,862,409 - PP_2515 0.47 -2.6 2,862,409 - PP_2515 0.47 +0.7 2,862,414 + PP_2515 0.47 -2.5 2,862,414 + PP_2515 0.47 +0.8 2,862,414 + PP_2515 0.47 +2.9 2,862,414 + PP_2515 0.47 -3.0 2,862,440 + PP_2515 0.51 -1.1 2,862,441 - PP_2515 0.51 +1.2 2,862,513 + PP_2515 0.60 +0.9 2,862,682 - PP_2515 0.82 +0.5 2,862,876 + +0.3 2,862,895 + -0.2 2,862,904 - +1.2 2,862,940 + -2.0 2,863,229 + PP_2516 0.24 -0.5 2,863,230 - PP_2516 0.24 -2.8 2,863,566 + PP_2516 0.52 -0.4 2,863,567 - PP_2516 0.52 -1.6 2,863,567 - PP_2516 0.52 -0.6 2,863,691 + PP_2516 0.63 +0.5
Or see this region's nucleotide sequence