Experiment: P. putida PUT 7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2420 and PP_2421 are separated by 413 nucleotides PP_2421 and PP_2422 are separated by 96 nucleotides PP_2422 and PP_2423 are separated by 108 nucleotides
PP_2420: PP_2420 - Outer membrane ferric siderophore receptor, at 2,766,533 to 2,768,719
_2420
PP_2421: PP_2421 - Acetyltransferase, GNAT family, at 2,769,133 to 2,769,684
_2421
PP_2422: PP_2422 - conserved protein of unknown function, at 2,769,781 to 2,770,134
_2422
PP_2423: PP_2423 - Fe(2+)/alpha-ketoglutarate-dependent dioxygenase, at 2,770,243 to 2,771,142
_2423
Position (kb)
2769
2770 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2768.274 kb on + strand, within PP_2420 at 2768.275 kb on - strand, within PP_2420 at 2768.651 kb on - strand at 2768.848 kb on + strand at 2768.848 kb on + strand at 2769.134 kb on + strand at 2769.230 kb on + strand, within PP_2421 at 2769.230 kb on + strand, within PP_2421 at 2769.230 kb on + strand, within PP_2421 at 2769.230 kb on + strand, within PP_2421 at 2769.230 kb on + strand, within PP_2421 at 2769.230 kb on + strand, within PP_2421 at 2769.230 kb on + strand, within PP_2421 at 2769.230 kb on + strand, within PP_2421 at 2769.231 kb on - strand, within PP_2421 at 2769.458 kb on + strand, within PP_2421 at 2769.459 kb on - strand, within PP_2421 at 2769.512 kb on + strand, within PP_2421 at 2769.512 kb on + strand, within PP_2421 at 2769.513 kb on - strand at 2769.513 kb on - strand, within PP_2421 at 2769.513 kb on - strand, within PP_2421 at 2769.513 kb on - strand, within PP_2421 at 2769.618 kb on - strand, within PP_2421 at 2769.824 kb on + strand, within PP_2422 at 2770.034 kb on + strand, within PP_2422 at 2770.035 kb on - strand, within PP_2422 at 2770.035 kb on - strand, within PP_2422 at 2770.087 kb on + strand, within PP_2422 at 2770.088 kb on - strand, within PP_2422 at 2770.088 kb on - strand, within PP_2422 at 2770.088 kb on - strand at 2770.095 kb on + strand, within PP_2422 at 2770.096 kb on - strand, within PP_2422 at 2770.250 kb on + strand at 2770.251 kb on - strand at 2770.298 kb on + strand at 2770.299 kb on - strand at 2770.299 kb on - strand at 2770.301 kb on + strand at 2770.301 kb on + strand at 2770.353 kb on - strand, within PP_2423 at 2770.470 kb on - strand, within PP_2423 at 2770.535 kb on + strand, within PP_2423 at 2770.535 kb on + strand, within PP_2423 at 2770.536 kb on - strand, within PP_2423 at 2770.536 kb on - strand, within PP_2423 at 2770.544 kb on + strand, within PP_2423 at 2770.611 kb on - strand, within PP_2423 at 2770.611 kb on - strand, within PP_2423 at 2770.650 kb on - strand, within PP_2423 at 2770.667 kb on + strand, within PP_2423 at 2770.668 kb on - strand, within PP_2423
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT 7 MOI 1 remove 2,768,274 + PP_2420 0.80 -2.8 2,768,275 - PP_2420 0.80 +0.7 2,768,651 - +0.5 2,768,848 + +0.4 2,768,848 + +0.1 2,769,134 + -2.6 2,769,230 + PP_2421 0.18 -0.3 2,769,230 + PP_2421 0.18 -1.2 2,769,230 + PP_2421 0.18 -0.6 2,769,230 + PP_2421 0.18 +0.6 2,769,230 + PP_2421 0.18 -0.3 2,769,230 + PP_2421 0.18 -1.7 2,769,230 + PP_2421 0.18 -0.7 2,769,230 + PP_2421 0.18 -1.6 2,769,231 - PP_2421 0.18 -1.4 2,769,458 + PP_2421 0.59 -1.2 2,769,459 - PP_2421 0.59 -1.4 2,769,512 + PP_2421 0.69 +0.1 2,769,512 + PP_2421 0.69 +0.4 2,769,513 - +0.4 2,769,513 - PP_2421 0.69 -1.2 2,769,513 - PP_2421 0.69 -2.4 2,769,513 - PP_2421 0.69 +0.8 2,769,618 - PP_2421 0.88 +1.2 2,769,824 + PP_2422 0.12 +1.3 2,770,034 + PP_2422 0.71 -1.9 2,770,035 - PP_2422 0.72 +0.7 2,770,035 - PP_2422 0.72 -0.1 2,770,087 + PP_2422 0.86 -1.2 2,770,088 - PP_2422 0.87 +0.4 2,770,088 - PP_2422 0.87 -1.6 2,770,088 - +0.4 2,770,095 + PP_2422 0.89 -0.3 2,770,096 - PP_2422 0.89 -0.1 2,770,250 + -1.9 2,770,251 - -2.5 2,770,298 + -1.4 2,770,299 - -0.9 2,770,299 - -0.9 2,770,301 + +1.4 2,770,301 + -2.0 2,770,353 - PP_2423 0.12 +0.5 2,770,470 - PP_2423 0.25 +0.3 2,770,535 + PP_2423 0.32 +0.7 2,770,535 + PP_2423 0.32 -0.6 2,770,536 - PP_2423 0.33 +1.7 2,770,536 - PP_2423 0.33 -0.5 2,770,544 + PP_2423 0.33 +0.9 2,770,611 - PP_2423 0.41 -0.1 2,770,611 - PP_2423 0.41 -3.2 2,770,650 - PP_2423 0.45 -0.6 2,770,667 + PP_2423 0.47 -1.9 2,770,668 - PP_2423 0.47 +0.1
Or see this region's nucleotide sequence