Experiment: P. putida PUT 7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2266 and PP_5507 are separated by 447 nucleotides PP_5507 and PP_2267 are separated by 60 nucleotides PP_2267 and PP_2268 are separated by 22 nucleotides PP_2268 and PP_2269 are separated by 2 nucleotides
PP_2266: PP_2266 - DNA-directed RNA polymerase, bacteriophage and mitochondrial type, at 2,586,636 to 2,589,194
_2266
PP_5507: PP_5507 - protein of unknown function, at 2,589,642 to 2,589,893
_5507
PP_2267: PP_2267 - putative Phage single-stranded DNA-binding protein, at 2,589,954 to 2,590,634
_2267
PP_2268: PP_2268 - Phage endodeoxyribonuclease I, at 2,590,657 to 2,591,082
_2268
PP_2269: PP_2269 - putative N-acetylmuramoyl-L-alanine amidase, at 2,591,085 to 2,591,534
_2269
Position (kb)
2589
2590
2591 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2589.048 kb on + strand at 2589.167 kb on - strand at 2589.215 kb on - strand at 2589.449 kb on + strand at 2589.449 kb on + strand at 2589.449 kb on + strand at 2589.450 kb on - strand at 2589.450 kb on - strand at 2589.504 kb on + strand at 2589.505 kb on - strand at 2589.522 kb on - strand at 2589.522 kb on - strand at 2589.605 kb on - strand at 2589.605 kb on - strand at 2589.605 kb on - strand at 2589.605 kb on - strand at 2589.659 kb on - strand at 2589.702 kb on - strand, within PP_5507 at 2589.765 kb on + strand, within PP_5507 at 2589.766 kb on - strand, within PP_5507 at 2589.766 kb on - strand, within PP_5507 at 2589.766 kb on - strand, within PP_5507 at 2589.821 kb on + strand, within PP_5507 at 2589.821 kb on + strand, within PP_5507 at 2589.822 kb on - strand, within PP_5507 at 2589.926 kb on + strand at 2589.927 kb on + strand at 2589.928 kb on - strand at 2589.949 kb on + strand at 2589.950 kb on - strand at 2590.041 kb on + strand, within PP_2267 at 2590.041 kb on + strand, within PP_2267 at 2590.041 kb on + strand, within PP_2267 at 2590.041 kb on + strand, within PP_2267 at 2590.042 kb on - strand, within PP_2267 at 2590.184 kb on - strand, within PP_2267 at 2590.184 kb on - strand, within PP_2267 at 2590.407 kb on + strand, within PP_2267 at 2590.408 kb on - strand, within PP_2267 at 2590.408 kb on - strand, within PP_2267 at 2590.408 kb on - strand, within PP_2267 at 2590.618 kb on - strand at 2590.669 kb on + strand at 2590.759 kb on + strand, within PP_2268 at 2591.080 kb on + strand at 2591.128 kb on - strand at 2591.296 kb on + strand, within PP_2269 at 2591.297 kb on - strand, within PP_2269 at 2591.297 kb on - strand, within PP_2269 at 2591.306 kb on + strand, within PP_2269 at 2591.307 kb on - strand, within PP_2269 at 2591.307 kb on - strand, within PP_2269 at 2591.508 kb on - strand at 2591.531 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT 7 MOI 1 remove 2,589,048 + +1.0 2,589,167 - +1.3 2,589,215 - -2.3 2,589,449 + -0.6 2,589,449 + -1.7 2,589,449 + +1.9 2,589,450 - -0.5 2,589,450 - -2.5 2,589,504 + -0.5 2,589,505 - +1.1 2,589,522 - -0.6 2,589,522 - +0.8 2,589,605 - -0.3 2,589,605 - -0.1 2,589,605 - +0.5 2,589,605 - +0.4 2,589,659 - +0.4 2,589,702 - PP_5507 0.24 -0.3 2,589,765 + PP_5507 0.49 -0.5 2,589,766 - PP_5507 0.49 -0.5 2,589,766 - PP_5507 0.49 +1.1 2,589,766 - PP_5507 0.49 +1.3 2,589,821 + PP_5507 0.71 -2.2 2,589,821 + PP_5507 0.71 +0.8 2,589,822 - PP_5507 0.71 +0.0 2,589,926 + +0.6 2,589,927 + -0.0 2,589,928 - +1.1 2,589,949 + -1.2 2,589,950 - -2.0 2,590,041 + PP_2267 0.13 -2.5 2,590,041 + PP_2267 0.13 +0.7 2,590,041 + PP_2267 0.13 +2.7 2,590,041 + PP_2267 0.13 -0.7 2,590,042 - PP_2267 0.13 +0.1 2,590,184 - PP_2267 0.34 -2.5 2,590,184 - PP_2267 0.34 +1.9 2,590,407 + PP_2267 0.67 +0.9 2,590,408 - PP_2267 0.67 -0.6 2,590,408 - PP_2267 0.67 -2.8 2,590,408 - PP_2267 0.67 +0.9 2,590,618 - +0.6 2,590,669 + +1.0 2,590,759 + PP_2268 0.24 +0.3 2,591,080 + -2.0 2,591,128 - -1.7 2,591,296 + PP_2269 0.47 -0.5 2,591,297 - PP_2269 0.47 +0.6 2,591,297 - PP_2269 0.47 +2.3 2,591,306 + PP_2269 0.49 -0.5 2,591,307 - PP_2269 0.49 +1.6 2,591,307 - PP_2269 0.49 -1.0 2,591,508 - -0.3 2,591,531 - +0.3
Or see this region's nucleotide sequence