Strain Fitness in Pseudomonas putida KT2440 around PP_1945

Experiment: P. putida PUT 7 MOI 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	P. putida PUT 7 MOI 1
2,199,471	+		PP_1944	0.26	+0.6
2,199,471	+		PP_1944	0.26	-0.4
2,199,493	-		PP_1944	0.28	-0.5
2,199,717	+		PP_1944	0.46	+1.3
2,199,718	-		PP_1944	0.46	+0.0
2,199,747	+		PP_1944	0.48	+0.8
2,199,747	+		PP_1944	0.48	+1.6
2,199,818	+		PP_1944	0.53	+0.7
2,199,818	+		PP_1944	0.53	-0.4
2,199,818	+		PP_1944	0.53	-0.1
2,199,819	-		PP_1944	0.54	+1.1
2,199,825	+		PP_1944	0.54	+0.0
2,199,825	+		PP_1944	0.54	-3.1
2,199,861	+		PP_1944	0.57	+0.7
2,199,861	+		PP_1944	0.57	+3.1
2,199,862	-		PP_1944	0.57	+1.0
2,199,936	-				+2.5
2,199,936	-		PP_1944	0.63	-1.5
2,199,940	+		PP_1944	0.63	+1.0
2,199,941	-		PP_1944	0.63	+1.0
2,200,007	+		PP_1944	0.68	+2.5
2,200,007	+		PP_1944	0.68	+0.6
2,200,132	-		PP_1944	0.78	-0.2
2,200,169	+		PP_1944	0.81	+2.3
2,200,169	+		PP_1944	0.81	+1.0
2,200,169	+		PP_1944	0.81	+1.0
2,200,170	-		PP_1944	0.81	-0.5
2,200,170	-		PP_1944	0.81	-0.2
2,200,170	-		PP_1944	0.81	+1.3
2,200,173	+		PP_1944	0.81	-1.1
2,200,173	+		PP_1944	0.81	+1.4
2,200,173	+		PP_1944	0.81	+0.3
2,200,173	+		PP_1944	0.81	+1.5
2,200,174	-		PP_1944	0.81	+0.0
2,200,214	+		PP_1944	0.84	-1.1
2,200,214	+		PP_1944	0.84	-0.0
2,200,214	+		PP_1944	0.84	+0.1
2,200,215	-		PP_1944	0.85	+1.0
2,200,215	-		PP_1944	0.85	+0.5
2,200,232	+		PP_1944	0.86	+0.8
2,200,232	+		PP_1944	0.86	+0.3
2,200,233	-		PP_1944	0.86	+1.6
2,200,233	-		PP_1944	0.86	-2.1
2,200,240	-		PP_1944	0.86	-1.9
2,200,371	+				-0.9
2,200,417	+				+0.1
2,200,417	+				-2.5
2,200,417	+				-0.5
2,200,418	-				+1.5
2,200,418	-				+0.6
2,200,482	+				+1.5
2,200,483	-				+0.1
2,200,483	-				-0.3
2,200,483	-				-2.2
2,200,483	-				-0.5
2,200,574	+		PP_1945	0.17	+0.1
2,200,574	+		PP_1945	0.17	+3.2
2,200,575	-		PP_1945	0.17	-1.1
2,200,575	-		PP_1945	0.17	+1.2
2,200,632	+		PP_1945	0.23	-0.1
2,200,633	-		PP_1945	0.24	-2.8
2,200,701	+		PP_1945	0.31	-0.0
2,200,701	+		PP_1945	0.31	+0.5
2,200,701	+		PP_1945	0.31	-0.3
2,200,702	-		PP_1945	0.31	+0.3
2,200,702	-		PP_1945	0.31	+0.1
2,200,702	-		PP_1945	0.31	+2.1
2,200,702	-		PP_1945	0.31	+1.4
2,200,702	-		PP_1945	0.31	+0.5
2,200,785	+		PP_1945	0.41	+0.6
2,200,785	+		PP_1945	0.41	-0.5
2,200,785	+		PP_1945	0.41	+2.9
2,200,785	+		PP_1945	0.41	+0.5
2,200,785	+		PP_1945	0.41	+0.5
2,200,785	+		PP_1945	0.41	-0.1
2,200,786	-		PP_1945	0.41	-1.1
2,200,786	-		PP_1945	0.41	+0.5
2,200,786	-				+3.1
2,200,786	-		PP_1945	0.41	+1.0
2,200,786	-		PP_1945	0.41	-0.0
2,200,786	-		PP_1945	0.41	+0.9
2,200,795	+		PP_1945	0.42	-1.9
2,200,843	+				+2.3
2,200,843	+		PP_1945	0.47	+0.9
2,200,843	+		PP_1945	0.47	-0.0
2,200,844	-		PP_1945	0.48	+0.9
2,200,844	-		PP_1945	0.48	+1.3
2,200,844	-		PP_1945	0.48	+1.6
2,201,043	+		PP_1945	0.70	+1.8
2,201,043	+		PP_1945	0.70	-2.0
2,201,044	-		PP_1945	0.70	+1.5
2,201,044	-		PP_1945	0.70	+1.9
2,201,044	-		PP_1945	0.70	+1.3
2,201,044	-		PP_1945	0.70	+0.3
2,201,044	-		PP_1945	0.70	-0.2
2,201,087	+		PP_1945	0.75	+0.8
2,201,087	+		PP_1945	0.75	+0.5
2,201,087	+		PP_1945	0.75	+1.6
2,201,087	+		PP_1945	0.75	-0.4
2,201,088	-		PP_1945	0.75	-2.0
2,201,088	-		PP_1945	0.75	+1.2
2,201,088	-		PP_1945	0.75	+0.5
2,201,088	-		PP_1945	0.75	+0.9
2,201,088	-		PP_1945	0.75	-0.6
2,201,089	+		PP_1945	0.76	+0.4
2,201,090	-		PP_1945	0.76	+0.7
2,201,090	-		PP_1945	0.76	+1.5
2,201,118	+		PP_1945	0.79	-0.5
2,201,209	+		PP_1945	0.89	-0.1
2,201,210	-		PP_1945	0.89	-0.1
2,201,210	-		PP_1945	0.89	+2.8
2,201,210	-		PP_1945	0.89	-0.1
2,201,249	+				+0.4
2,201,249	+				+2.1
2,201,249	+				+2.1
2,201,249	+				+1.1
2,201,250	-				+1.1
2,201,250	-				-0.3
2,201,263	+				+0.2
2,201,263	+				+1.7
2,201,263	+				+1.1
2,201,296	+				-1.1
2,201,296	+				-1.8
2,201,296	+				+0.1
2,201,296	+				+0.6
2,201,296	+				+0.8
2,201,297	-				+1.1
2,201,334	+				-0.4
2,201,336	+				+3.7
2,201,354	+				+1.8
2,201,355	-				+2.0
2,201,355	-				-2.4
2,201,554	+		PP_1946	0.25	+0.3
2,201,555	-		PP_1946	0.25	+0.4
2,201,555	-		PP_1946	0.25	+0.0
2,201,652	-		PP_1946	0.38	-1.1
2,201,724	+		PP_1946	0.47	+1.4
2,201,724	+		PP_1946	0.47	+1.1
2,201,724	+		PP_1946	0.47	+2.5
2,201,725	-		PP_1946	0.47	-0.5
2,201,725	-		PP_1946	0.47	-2.0
2,201,725	-		PP_1946	0.47	+0.4
2,201,725	-		PP_1946	0.47	+1.1
2,201,725	-		PP_1946	0.47	+0.4
2,201,829	+		PP_1946	0.60	+0.6
2,201,851	+		PP_1946	0.63	-1.8
2,201,883	+		PP_1946	0.67	+0.1
2,201,883	+		PP_1946	0.67	+1.5
2,201,898	-		PP_1946	0.69	+1.3
2,201,906	+		PP_1946	0.70	+1.0
2,201,907	-		PP_1946	0.70	-1.0
2,202,063	+		PP_1946	0.90	+1.6
2,202,151	+				+0.8
2,202,270	-				+0.4