Experiment: P. putida PUT 7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1772 and PP_1773 are separated by 299 nucleotides PP_1773 and PP_1774 overlap by 32 nucleotides PP_1774 and PP_1775 are separated by 102 nucleotides PP_1775 and PP_1776 are separated by 327 nucleotides
PP_1772: PP_1772 - 30S ribosomal protein S1, at 1,979,331 to 1,981,061
_1772
PP_1773: PP_1773 - Integration host factor subunit beta, at 1,981,361 to 1,981,663
_1773
PP_1774: PP_1774 - conserved exported protein of unknown function, at 1,981,632 to 1,981,946
_1774
PP_1775: PP_1775 - Metallo-beta-lactamase family protein, at 1,982,049 to 1,983,482
_1775
PP_1776: PP_1776 - Mannose-6-phosphate isomerase/mannose-1-phosphate guanylyltransferase, at 1,983,810 to 1,985,252
_1776
Position (kb)
1982
1983
1984 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7 at 1981.183 kb on - strand at 1981.221 kb on - strand at 1981.221 kb on - strand at 1981.224 kb on + strand at 1981.225 kb on - strand at 1981.225 kb on - strand at 1981.231 kb on + strand at 1981.271 kb on + strand at 1981.272 kb on - strand at 1981.360 kb on + strand at 1981.387 kb on + strand at 1981.387 kb on + strand at 1981.387 kb on + strand at 1981.388 kb on - strand at 1981.388 kb on - strand at 1981.726 kb on + strand, within PP_1774 at 1981.727 kb on - strand, within PP_1774 at 1981.727 kb on - strand, within PP_1774 at 1981.945 kb on - strand at 1981.945 kb on - strand at 1981.963 kb on + strand at 1981.963 kb on + strand at 1981.964 kb on - strand at 1981.964 kb on - strand at 1981.964 kb on - strand at 1981.964 kb on - strand at 1981.966 kb on + strand at 1981.967 kb on - strand at 1981.982 kb on + strand at 1981.982 kb on + strand at 1981.983 kb on - strand at 1981.983 kb on - strand at 1981.983 kb on - strand at 1981.983 kb on - strand at 1982.007 kb on + strand at 1982.008 kb on - strand at 1982.008 kb on - strand at 1982.008 kb on - strand at 1982.010 kb on + strand at 1982.011 kb on - strand at 1982.011 kb on - strand at 1982.052 kb on + strand at 1982.304 kb on - strand, within PP_1775 at 1982.359 kb on - strand, within PP_1775 at 1982.359 kb on - strand, within PP_1775 at 1982.401 kb on - strand, within PP_1775 at 1982.423 kb on + strand, within PP_1775 at 1982.424 kb on - strand, within PP_1775 at 1982.487 kb on - strand, within PP_1775 at 1982.526 kb on + strand, within PP_1775 at 1982.527 kb on - strand, within PP_1775 at 1982.527 kb on - strand, within PP_1775 at 1982.540 kb on + strand, within PP_1775 at 1982.569 kb on - strand, within PP_1775 at 1982.569 kb on - strand, within PP_1775 at 1982.664 kb on + strand, within PP_1775 at 1982.796 kb on + strand, within PP_1775 at 1983.057 kb on + strand, within PP_1775 at 1983.057 kb on + strand, within PP_1775 at 1983.132 kb on + strand, within PP_1775 at 1983.133 kb on - strand, within PP_1775 at 1983.212 kb on - strand, within PP_1775 at 1983.271 kb on - strand, within PP_1775 at 1983.271 kb on - strand, within PP_1775 at 1983.450 kb on - strand at 1983.470 kb on + strand at 1983.470 kb on + strand at 1983.470 kb on + strand at 1983.471 kb on - strand at 1983.471 kb on - strand at 1983.471 kb on - strand at 1983.471 kb on - strand at 1983.471 kb on - strand at 1983.481 kb on - strand at 1983.564 kb on + strand at 1983.565 kb on - strand at 1983.625 kb on + strand at 1983.625 kb on + strand at 1983.625 kb on + strand at 1983.625 kb on + strand at 1983.625 kb on + strand at 1983.626 kb on - strand at 1983.626 kb on - strand at 1983.626 kb on - strand at 1983.644 kb on + strand at 1983.644 kb on + strand at 1983.650 kb on - strand at 1983.710 kb on - strand at 1983.710 kb on - strand at 1983.713 kb on + strand at 1983.714 kb on - strand at 1983.748 kb on + strand at 1983.749 kb on - strand at 1983.751 kb on + strand at 1983.751 kb on + strand at 1983.751 kb on + strand at 1983.752 kb on - strand at 1983.752 kb on - strand at 1983.756 kb on + strand at 1983.756 kb on + strand at 1983.756 kb on + strand at 1983.756 kb on + strand at 1983.757 kb on - strand at 1983.757 kb on - strand at 1983.757 kb on - strand at 1983.865 kb on + strand at 1983.866 kb on - strand at 1984.053 kb on + strand, within PP_1776 at 1984.053 kb on + strand, within PP_1776 at 1984.053 kb on + strand, within PP_1776 at 1984.253 kb on + strand, within PP_1776 at 1984.254 kb on - strand, within PP_1776 at 1984.254 kb on - strand, within PP_1776 at 1984.264 kb on - strand, within PP_1776 at 1984.284 kb on + strand, within PP_1776 at 1984.284 kb on + strand, within PP_1776 at 1984.285 kb on - strand, within PP_1776 at 1984.324 kb on + strand, within PP_1776 at 1984.325 kb on - strand, within PP_1776 at 1984.325 kb on - strand, within PP_1776 at 1984.374 kb on + strand, within PP_1776 at 1984.375 kb on - strand, within PP_1776 at 1984.375 kb on - strand, within PP_1776 at 1984.375 kb on - strand, within PP_1776 at 1984.375 kb on - strand, within PP_1776 at 1984.375 kb on - strand, within PP_1776 at 1984.392 kb on + strand, within PP_1776 at 1984.393 kb on - strand, within PP_1776 at 1984.455 kb on + strand, within PP_1776 at 1984.455 kb on + strand, within PP_1776 at 1984.455 kb on + strand, within PP_1776 at 1984.456 kb on - strand at 1984.456 kb on - strand, within PP_1776
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT 7 MOI 1 remove 1,981,183 - -1.2 1,981,221 - +0.3 1,981,221 - -0.1 1,981,224 + -1.8 1,981,225 - +2.4 1,981,225 - +1.7 1,981,231 + -0.2 1,981,271 + +0.0 1,981,272 - +0.8 1,981,360 + -1.8 1,981,387 + -1.1 1,981,387 + -2.4 1,981,387 + -1.3 1,981,388 - -3.1 1,981,388 - -0.6 1,981,726 + PP_1774 0.30 +0.6 1,981,727 - PP_1774 0.30 +0.5 1,981,727 - PP_1774 0.30 +1.4 1,981,945 - -1.5 1,981,945 - +0.0 1,981,963 + +0.7 1,981,963 + -1.2 1,981,964 - +1.5 1,981,964 - +1.1 1,981,964 - +0.1 1,981,964 - +1.6 1,981,966 + -1.6 1,981,967 - +0.3 1,981,982 + +0.8 1,981,982 + -2.4 1,981,983 - +0.1 1,981,983 - +1.4 1,981,983 - -0.6 1,981,983 - +1.0 1,982,007 + -2.4 1,982,008 - +1.4 1,982,008 - -0.5 1,982,008 - +0.5 1,982,010 + +0.1 1,982,011 - +0.3 1,982,011 - -1.5 1,982,052 + -1.3 1,982,304 - PP_1775 0.18 -2.7 1,982,359 - PP_1775 0.22 +0.9 1,982,359 - PP_1775 0.22 +0.9 1,982,401 - PP_1775 0.25 -2.3 1,982,423 + PP_1775 0.26 -2.2 1,982,424 - PP_1775 0.26 +0.6 1,982,487 - PP_1775 0.31 -0.6 1,982,526 + PP_1775 0.33 -0.1 1,982,527 - PP_1775 0.33 +1.4 1,982,527 - PP_1775 0.33 +1.3 1,982,540 + PP_1775 0.34 +0.7 1,982,569 - PP_1775 0.36 +0.3 1,982,569 - PP_1775 0.36 +1.4 1,982,664 + PP_1775 0.43 +1.0 1,982,796 + PP_1775 0.52 +1.1 1,983,057 + PP_1775 0.70 +0.6 1,983,057 + PP_1775 0.70 +1.0 1,983,132 + PP_1775 0.76 +1.4 1,983,133 - PP_1775 0.76 +4.0 1,983,212 - PP_1775 0.81 +4.7 1,983,271 - PP_1775 0.85 +5.3 1,983,271 - PP_1775 0.85 +6.2 1,983,450 - +4.3 1,983,470 + -2.3 1,983,470 + +0.1 1,983,470 + +0.2 1,983,471 - +7.5 1,983,471 - +5.9 1,983,471 - +4.9 1,983,471 - +5.2 1,983,471 - +3.9 1,983,481 - +5.5 1,983,564 + -0.9 1,983,565 - +4.4 1,983,625 + +1.3 1,983,625 + -0.2 1,983,625 + -1.5 1,983,625 + +0.4 1,983,625 + +1.1 1,983,626 - +6.1 1,983,626 - +6.6 1,983,626 - +5.2 1,983,644 + +0.8 1,983,644 + +0.5 1,983,650 - +4.9 1,983,710 - +5.6 1,983,710 - +6.2 1,983,713 + -1.4 1,983,714 - +6.9 1,983,748 + +4.9 1,983,749 - +5.9 1,983,751 + +6.0 1,983,751 + +5.1 1,983,751 + +5.1 1,983,752 - +6.0 1,983,752 - +6.1 1,983,756 + +6.1 1,983,756 + +6.0 1,983,756 + +5.0 1,983,756 + +4.8 1,983,757 - +5.3 1,983,757 - +7.0 1,983,757 - +6.9 1,983,865 + +6.5 1,983,866 - +6.3 1,984,053 + PP_1776 0.17 +6.9 1,984,053 + PP_1776 0.17 +5.8 1,984,053 + PP_1776 0.17 +5.9 1,984,253 + PP_1776 0.31 +5.3 1,984,254 - PP_1776 0.31 +6.4 1,984,254 - PP_1776 0.31 +5.0 1,984,264 - PP_1776 0.31 +6.3 1,984,284 + PP_1776 0.33 +6.1 1,984,284 + PP_1776 0.33 +5.7 1,984,285 - PP_1776 0.33 +6.4 1,984,324 + PP_1776 0.36 +6.0 1,984,325 - PP_1776 0.36 +5.9 1,984,325 - PP_1776 0.36 +5.8 1,984,374 + PP_1776 0.39 +5.3 1,984,375 - PP_1776 0.39 +6.5 1,984,375 - PP_1776 0.39 +6.3 1,984,375 - PP_1776 0.39 +5.5 1,984,375 - PP_1776 0.39 +6.7 1,984,375 - PP_1776 0.39 +6.4 1,984,392 + PP_1776 0.40 +5.7 1,984,393 - PP_1776 0.40 +6.8 1,984,455 + PP_1776 0.45 +6.4 1,984,455 + PP_1776 0.45 +6.3 1,984,455 + PP_1776 0.45 +3.9 1,984,456 - +5.2 1,984,456 - PP_1776 0.45 +6.7
Or see this region's nucleotide sequence