Experiment: P. putida PUT 7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0156 and PP_0157 are separated by 419 nucleotides PP_0157 and PP_0158 are separated by 160 nucleotides
PP_0156: PP_0156 - pyridine nucleotide transhydrogenase subunit alpha, at 165,785 to 166,906
_0156
PP_0157: PP_0157 - transcriptional activator of gcdH, LysR family, at 167,326 to 168,243
_0157
PP_0158: PP_0158 - glutaryl-CoA dehydrogenase, at 168,404 to 169,585
_0158
Position (kb) 167
168
169 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 166.400 kb on + strand, within PP_0156 at 166.464 kb on + strand, within PP_0156 at 166.464 kb on + strand, within PP_0156 at 166.465 kb on - strand, within PP_0156 at 166.465 kb on - strand, within PP_0156 at 166.465 kb on - strand, within PP_0156 at 166.494 kb on + strand, within PP_0156 at 166.494 kb on + strand, within PP_0156 at 166.495 kb on - strand, within PP_0156 at 166.495 kb on - strand, within PP_0156 at 166.580 kb on - strand, within PP_0156 at 166.700 kb on + strand, within PP_0156 at 166.716 kb on + strand, within PP_0156 at 166.716 kb on + strand, within PP_0156 at 166.910 kb on + strand at 166.910 kb on + strand at 166.910 kb on + strand at 166.910 kb on + strand at 166.910 kb on + strand at 166.911 kb on - strand at 166.911 kb on - strand at 166.911 kb on - strand at 166.940 kb on + strand at 166.941 kb on - strand at 166.941 kb on - strand at 167.076 kb on + strand at 167.076 kb on + strand at 167.185 kb on - strand at 167.191 kb on - strand at 167.208 kb on + strand at 167.225 kb on + strand at 167.239 kb on - strand at 167.285 kb on - strand at 167.285 kb on - strand at 167.285 kb on - strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.287 kb on - strand at 167.287 kb on - strand at 167.287 kb on - strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.298 kb on + strand at 167.307 kb on - strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.353 kb on - strand at 167.353 kb on - strand at 167.353 kb on - strand at 167.353 kb on - strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.360 kb on + strand at 167.360 kb on + strand at 167.361 kb on - strand at 167.361 kb on - strand at 167.378 kb on - strand at 167.441 kb on + strand, within PP_0157 at 167.441 kb on + strand, within PP_0157 at 167.442 kb on - strand, within PP_0157 at 167.442 kb on - strand, within PP_0157 at 167.442 kb on - strand, within PP_0157 at 167.445 kb on - strand, within PP_0157 at 167.445 kb on - strand, within PP_0157 at 167.445 kb on - strand, within PP_0157 at 167.458 kb on - strand, within PP_0157 at 167.476 kb on - strand, within PP_0157 at 167.527 kb on + strand, within PP_0157 at 167.528 kb on - strand, within PP_0157 at 167.578 kb on + strand, within PP_0157 at 167.578 kb on + strand, within PP_0157 at 167.578 kb on + strand, within PP_0157 at 167.579 kb on - strand, within PP_0157 at 167.579 kb on - strand, within PP_0157 at 167.585 kb on + strand, within PP_0157 at 167.586 kb on - strand, within PP_0157 at 167.807 kb on + strand, within PP_0157 at 167.808 kb on - strand, within PP_0157 at 167.808 kb on - strand, within PP_0157 at 167.827 kb on - strand, within PP_0157 at 167.827 kb on - strand, within PP_0157 at 167.914 kb on + strand, within PP_0157 at 167.915 kb on - strand, within PP_0157 at 167.994 kb on + strand, within PP_0157 at 167.994 kb on + strand, within PP_0157 at 167.995 kb on - strand, within PP_0157 at 168.039 kb on - strand, within PP_0157 at 168.099 kb on + strand, within PP_0157 at 168.174 kb on + strand at 168.245 kb on + strand at 168.262 kb on + strand at 168.263 kb on - strand at 168.263 kb on - strand at 168.263 kb on - strand at 168.263 kb on - strand at 168.274 kb on + strand at 168.275 kb on - strand at 168.275 kb on - strand at 168.358 kb on - strand at 168.386 kb on + strand at 168.386 kb on + strand at 168.621 kb on - strand, within PP_0158 at 168.641 kb on + strand, within PP_0158 at 168.641 kb on + strand, within PP_0158 at 168.641 kb on + strand, within PP_0158 at 168.641 kb on + strand, within PP_0158 at 168.641 kb on + strand, within PP_0158 at 168.641 kb on + strand, within PP_0158 at 168.642 kb on - strand, within PP_0158 at 168.642 kb on - strand, within PP_0158 at 168.642 kb on - strand, within PP_0158 at 168.642 kb on - strand, within PP_0158 at 168.642 kb on - strand, within PP_0158 at 168.650 kb on + strand, within PP_0158 at 168.650 kb on + strand, within PP_0158 at 168.650 kb on + strand, within PP_0158 at 168.651 kb on - strand, within PP_0158 at 168.658 kb on + strand, within PP_0158 at 168.773 kb on + strand, within PP_0158 at 168.829 kb on + strand, within PP_0158 at 168.830 kb on - strand, within PP_0158 at 169.160 kb on + strand, within PP_0158 at 169.160 kb on + strand, within PP_0158 at 169.160 kb on + strand, within PP_0158 at 169.160 kb on + strand, within PP_0158 at 169.161 kb on - strand, within PP_0158 at 169.165 kb on + strand, within PP_0158 at 169.220 kb on + strand, within PP_0158 at 169.220 kb on + strand, within PP_0158 at 169.220 kb on + strand, within PP_0158 at 169.221 kb on - strand, within PP_0158
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT 7 MOI 1 remove 166,400 + PP_0156 0.55 +2.3 166,464 + PP_0156 0.61 +0.3 166,464 + PP_0156 0.61 +0.7 166,465 - PP_0156 0.61 +1.9 166,465 - PP_0156 0.61 +2.6 166,465 - PP_0156 0.61 +0.8 166,494 + PP_0156 0.63 +2.0 166,494 + PP_0156 0.63 +1.6 166,495 - PP_0156 0.63 -0.3 166,495 - PP_0156 0.63 +2.6 166,580 - PP_0156 0.71 +0.9 166,700 + PP_0156 0.82 +1.3 166,716 + PP_0156 0.83 -1.8 166,716 + PP_0156 0.83 +0.7 166,910 + -0.8 166,910 + +0.8 166,910 + +1.7 166,910 + +1.4 166,910 + +1.1 166,911 - -0.6 166,911 - +2.4 166,911 - +1.0 166,940 + +1.3 166,941 - +0.7 166,941 - +2.3 167,076 + -2.4 167,076 + +1.5 167,185 - -1.5 167,191 - +1.4 167,208 + -0.3 167,225 + -3.4 167,239 - +2.9 167,285 - +1.4 167,285 - +1.0 167,285 - +0.9 167,286 + -0.0 167,286 + +1.2 167,286 + +1.3 167,286 + +0.9 167,286 + +1.1 167,286 + +0.5 167,286 + +0.7 167,286 + +3.3 167,286 + +1.3 167,287 - +1.6 167,287 - +1.3 167,287 - +1.3 167,290 + -0.7 167,290 + +0.9 167,290 + +0.1 167,290 + +0.8 167,290 + -0.7 167,290 + +0.8 167,290 + +1.5 167,290 + +1.5 167,290 + +1.5 167,290 + +1.3 167,290 + +1.6 167,290 + +0.8 167,290 + +0.7 167,290 + +3.9 167,290 + +1.3 167,290 + +2.3 167,291 - -0.1 167,291 - +1.7 167,291 - +0.4 167,291 - +1.7 167,291 - +0.0 167,291 - +0.9 167,291 - +2.0 167,291 - +3.7 167,291 - -2.1 167,291 - -0.0 167,298 + +0.3 167,307 - -1.3 167,352 + +1.9 167,352 + -0.7 167,352 + +1.2 167,352 + +1.6 167,352 + +1.6 167,353 - +0.4 167,353 - +0.4 167,353 - +0.1 167,353 - -0.1 167,356 + +2.5 167,356 + +2.2 167,356 + +1.0 167,356 + +0.7 167,356 + -0.7 167,356 + -1.5 167,357 - +1.3 167,357 - -1.8 167,357 - -2.2 167,357 - +1.4 167,357 - +0.9 167,357 - +1.0 167,360 + +0.9 167,360 + +1.0 167,361 - +0.3 167,361 - +1.4 167,378 - -0.0 167,441 + PP_0157 0.13 +1.9 167,441 + PP_0157 0.13 -0.3 167,442 - PP_0157 0.13 +1.2 167,442 - PP_0157 0.13 +2.0 167,442 - PP_0157 0.13 +2.9 167,445 - PP_0157 0.13 +1.6 167,445 - PP_0157 0.13 -1.1 167,445 - PP_0157 0.13 +1.1 167,458 - PP_0157 0.14 +1.8 167,476 - PP_0157 0.16 -0.6 167,527 + PP_0157 0.22 +0.3 167,528 - PP_0157 0.22 +0.8 167,578 + PP_0157 0.27 -2.0 167,578 + PP_0157 0.27 +2.5 167,578 + PP_0157 0.27 +1.4 167,579 - PP_0157 0.28 +0.2 167,579 - PP_0157 0.28 -0.8 167,585 + PP_0157 0.28 +0.3 167,586 - PP_0157 0.28 +1.0 167,807 + PP_0157 0.52 +1.3 167,808 - PP_0157 0.53 +0.8 167,808 - PP_0157 0.53 -0.7 167,827 - PP_0157 0.55 -0.7 167,827 - PP_0157 0.55 +0.5 167,914 + PP_0157 0.64 +1.8 167,915 - PP_0157 0.64 +0.9 167,994 + PP_0157 0.73 +0.4 167,994 + PP_0157 0.73 -0.3 167,995 - PP_0157 0.73 +1.9 168,039 - PP_0157 0.78 +1.3 168,099 + PP_0157 0.84 -0.5 168,174 + +0.3 168,245 + +0.4 168,262 + -1.7 168,263 - +2.6 168,263 - -0.4 168,263 - -2.8 168,263 - +2.8 168,274 + +2.5 168,275 - +0.8 168,275 - +0.1 168,358 - -0.5 168,386 + +0.8 168,386 + +0.3 168,621 - PP_0158 0.18 +1.9 168,641 + PP_0158 0.20 +0.3 168,641 + PP_0158 0.20 +1.1 168,641 + PP_0158 0.20 +1.4 168,641 + PP_0158 0.20 +0.8 168,641 + PP_0158 0.20 +0.9 168,641 + PP_0158 0.20 +0.8 168,642 - PP_0158 0.20 -0.8 168,642 - PP_0158 0.20 +1.1 168,642 - PP_0158 0.20 +1.6 168,642 - PP_0158 0.20 -0.7 168,642 - PP_0158 0.20 +1.9 168,650 + PP_0158 0.21 +1.7 168,650 + PP_0158 0.21 +2.1 168,650 + PP_0158 0.21 +1.0 168,651 - PP_0158 0.21 +0.7 168,658 + PP_0158 0.21 -1.1 168,773 + PP_0158 0.31 +0.8 168,829 + PP_0158 0.36 +1.5 168,830 - PP_0158 0.36 -0.1 169,160 + PP_0158 0.64 +1.7 169,160 + PP_0158 0.64 -2.1 169,160 + PP_0158 0.64 +2.4 169,160 + PP_0158 0.64 -0.7 169,161 - PP_0158 0.64 +0.5 169,165 + PP_0158 0.64 +2.4 169,220 + PP_0158 0.69 -0.5 169,220 + PP_0158 0.69 +1.4 169,220 + PP_0158 0.69 +0.9 169,221 - PP_0158 0.69 +1.1
Or see this region's nucleotide sequence