Experiment: P. putida PUT 7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0131 and PP_0132 are separated by 14 nucleotides PP_0132 and PP_0133 overlap by 4 nucleotides
PP_0131: PP_0131 - putative membrane-anchored phosphodiesterase, at 137,100 to 138,707
_0131
PP_0132: PP_0132 - Alginate biosynthesis sensor protein KinB, at 138,722 to 140,503
_0132
PP_0133: PP_0133 - Alginate biosynthesis transcriptional regulatory protein AlgB, at 140,500 to 141,846
_0133
Position (kb) 138
139
140
141 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 137.799 kb on + strand, within PP_0131 at 137.912 kb on - strand, within PP_0131 at 137.992 kb on - strand, within PP_0131 at 138.175 kb on + strand, within PP_0131 at 138.175 kb on + strand, within PP_0131 at 138.176 kb on - strand, within PP_0131 at 138.176 kb on - strand, within PP_0131 at 138.176 kb on - strand, within PP_0131 at 138.196 kb on + strand, within PP_0131 at 138.196 kb on + strand, within PP_0131 at 138.196 kb on + strand, within PP_0131 at 138.197 kb on - strand, within PP_0131 at 138.197 kb on - strand, within PP_0131 at 138.197 kb on - strand, within PP_0131 at 138.198 kb on + strand, within PP_0131 at 138.198 kb on + strand, within PP_0131 at 138.198 kb on + strand, within PP_0131 at 138.198 kb on + strand, within PP_0131 at 138.198 kb on + strand, within PP_0131 at 138.198 kb on + strand, within PP_0131 at 138.198 kb on + strand, within PP_0131 at 138.198 kb on + strand, within PP_0131 at 138.198 kb on + strand, within PP_0131 at 138.198 kb on + strand, within PP_0131 at 138.198 kb on + strand, within PP_0131 at 138.198 kb on + strand, within PP_0131 at 138.199 kb on - strand, within PP_0131 at 138.199 kb on - strand, within PP_0131 at 138.199 kb on - strand, within PP_0131 at 138.199 kb on - strand, within PP_0131 at 138.199 kb on - strand, within PP_0131 at 138.199 kb on - strand, within PP_0131 at 138.199 kb on - strand, within PP_0131 at 138.199 kb on - strand, within PP_0131 at 138.199 kb on - strand, within PP_0131 at 138.199 kb on - strand, within PP_0131 at 138.199 kb on - strand, within PP_0131 at 138.202 kb on + strand, within PP_0131 at 138.202 kb on + strand, within PP_0131 at 138.203 kb on - strand at 138.203 kb on - strand, within PP_0131 at 138.243 kb on - strand, within PP_0131 at 138.243 kb on - strand, within PP_0131 at 138.243 kb on - strand, within PP_0131 at 138.243 kb on - strand, within PP_0131 at 138.243 kb on - strand, within PP_0131 at 138.243 kb on - strand, within PP_0131 at 138.365 kb on - strand, within PP_0131 at 138.365 kb on - strand, within PP_0131 at 138.365 kb on - strand at 138.411 kb on + strand, within PP_0131 at 138.412 kb on - strand, within PP_0131 at 138.426 kb on + strand, within PP_0131 at 138.521 kb on + strand, within PP_0131 at 138.522 kb on - strand, within PP_0131 at 138.522 kb on - strand, within PP_0131 at 138.618 kb on + strand at 138.618 kb on + strand at 138.618 kb on + strand at 138.619 kb on - strand at 138.619 kb on - strand at 138.706 kb on - strand at 138.741 kb on + strand at 138.742 kb on - strand at 138.855 kb on - strand at 138.855 kb on - strand at 138.894 kb on + strand at 138.985 kb on + strand, within PP_0132 at 139.228 kb on + strand, within PP_0132 at 139.229 kb on - strand, within PP_0132 at 139.479 kb on + strand, within PP_0132 at 139.480 kb on - strand, within PP_0132 at 139.480 kb on - strand, within PP_0132 at 139.480 kb on - strand, within PP_0132 at 139.480 kb on - strand, within PP_0132 at 139.949 kb on - strand, within PP_0132 at 139.958 kb on + strand, within PP_0132 at 139.959 kb on - strand, within PP_0132 at 139.988 kb on + strand, within PP_0132 at 139.989 kb on - strand, within PP_0132 at 140.154 kb on + strand, within PP_0132 at 140.155 kb on - strand, within PP_0132 at 140.176 kb on - strand, within PP_0132 at 140.389 kb on + strand at 140.389 kb on + strand at 140.390 kb on - strand at 140.390 kb on - strand at 140.390 kb on - strand at 140.390 kb on - strand at 140.503 kb on + strand at 140.510 kb on + strand at 140.511 kb on - strand at 140.511 kb on - strand at 140.511 kb on - strand at 140.511 kb on - strand at 140.511 kb on - strand at 140.511 kb on - strand at 140.528 kb on + strand at 140.535 kb on + strand at 140.535 kb on + strand at 140.605 kb on - strand at 140.632 kb on - strand at 140.644 kb on + strand, within PP_0133 at 140.737 kb on + strand, within PP_0133 at 140.738 kb on - strand, within PP_0133 at 140.774 kb on + strand, within PP_0133 at 140.829 kb on - strand, within PP_0133 at 140.930 kb on + strand, within PP_0133 at 141.000 kb on + strand, within PP_0133 at 141.000 kb on + strand, within PP_0133 at 141.001 kb on - strand, within PP_0133 at 141.001 kb on - strand, within PP_0133 at 141.004 kb on + strand, within PP_0133 at 141.004 kb on + strand, within PP_0133 at 141.004 kb on + strand, within PP_0133 at 141.004 kb on + strand, within PP_0133 at 141.004 kb on + strand, within PP_0133 at 141.004 kb on + strand, within PP_0133 at 141.005 kb on - strand, within PP_0133 at 141.005 kb on - strand, within PP_0133 at 141.005 kb on - strand, within PP_0133 at 141.005 kb on - strand, within PP_0133 at 141.005 kb on - strand, within PP_0133 at 141.045 kb on - strand, within PP_0133 at 141.045 kb on - strand, within PP_0133 at 141.119 kb on - strand, within PP_0133 at 141.199 kb on + strand, within PP_0133 at 141.200 kb on - strand, within PP_0133 at 141.200 kb on - strand, within PP_0133 at 141.396 kb on + strand, within PP_0133 at 141.396 kb on + strand, within PP_0133 at 141.397 kb on - strand, within PP_0133 at 141.427 kb on + strand, within PP_0133 at 141.428 kb on - strand, within PP_0133
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida PUT 7 MOI 1 remove 137,799 + PP_0131 0.43 +1.2 137,912 - PP_0131 0.50 +1.1 137,992 - PP_0131 0.55 -0.8 138,175 + PP_0131 0.67 +1.0 138,175 + PP_0131 0.67 -0.7 138,176 - PP_0131 0.67 -2.5 138,176 - PP_0131 0.67 -0.9 138,176 - PP_0131 0.67 +0.0 138,196 + PP_0131 0.68 +1.2 138,196 + PP_0131 0.68 +0.9 138,196 + PP_0131 0.68 +1.3 138,197 - PP_0131 0.68 -0.7 138,197 - PP_0131 0.68 +1.2 138,197 - PP_0131 0.68 +0.4 138,198 + PP_0131 0.68 +0.1 138,198 + PP_0131 0.68 -0.7 138,198 + PP_0131 0.68 -2.1 138,198 + PP_0131 0.68 +0.1 138,198 + PP_0131 0.68 +1.2 138,198 + PP_0131 0.68 +2.1 138,198 + PP_0131 0.68 -0.9 138,198 + PP_0131 0.68 -0.1 138,198 + PP_0131 0.68 -0.8 138,198 + PP_0131 0.68 +2.0 138,198 + PP_0131 0.68 +1.1 138,198 + PP_0131 0.68 +0.5 138,199 - PP_0131 0.68 +0.1 138,199 - PP_0131 0.68 +1.1 138,199 - PP_0131 0.68 +0.5 138,199 - PP_0131 0.68 -1.3 138,199 - PP_0131 0.68 +1.0 138,199 - PP_0131 0.68 +1.8 138,199 - PP_0131 0.68 +0.1 138,199 - PP_0131 0.68 +0.1 138,199 - PP_0131 0.68 -0.4 138,199 - PP_0131 0.68 +0.9 138,199 - PP_0131 0.68 +1.2 138,202 + PP_0131 0.69 +0.2 138,202 + PP_0131 0.69 +1.1 138,203 - -0.7 138,203 - PP_0131 0.69 +1.2 138,243 - PP_0131 0.71 +0.5 138,243 - PP_0131 0.71 +1.3 138,243 - PP_0131 0.71 +1.0 138,243 - PP_0131 0.71 -0.9 138,243 - PP_0131 0.71 +0.5 138,243 - PP_0131 0.71 -1.1 138,365 - PP_0131 0.79 -3.5 138,365 - PP_0131 0.79 -1.4 138,365 - -0.7 138,411 + PP_0131 0.82 -0.3 138,412 - PP_0131 0.82 +1.4 138,426 + PP_0131 0.82 +1.1 138,521 + PP_0131 0.88 +1.2 138,522 - PP_0131 0.88 -0.4 138,522 - PP_0131 0.88 +0.5 138,618 + +1.6 138,618 + -0.7 138,618 + +2.0 138,619 - +0.8 138,619 - +1.3 138,706 - +1.2 138,741 + +0.8 138,742 - +0.8 138,855 - -0.0 138,855 - +0.0 138,894 + -0.6 138,985 + PP_0132 0.15 +2.0 139,228 + PP_0132 0.28 +0.3 139,229 - PP_0132 0.28 -2.1 139,479 + PP_0132 0.42 +1.6 139,480 - PP_0132 0.43 -0.4 139,480 - PP_0132 0.43 +1.2 139,480 - PP_0132 0.43 +1.7 139,480 - PP_0132 0.43 +0.5 139,949 - PP_0132 0.69 +0.2 139,958 + PP_0132 0.69 +1.2 139,959 - PP_0132 0.69 +1.8 139,988 + PP_0132 0.71 +0.6 139,989 - PP_0132 0.71 +0.2 140,154 + PP_0132 0.80 +2.3 140,155 - PP_0132 0.80 -2.3 140,176 - PP_0132 0.82 -0.3 140,389 + +0.6 140,389 + -0.0 140,390 - +0.6 140,390 - +2.1 140,390 - -0.9 140,390 - -1.3 140,503 + -0.1 140,510 + -0.1 140,511 - +0.1 140,511 - +0.4 140,511 - +0.3 140,511 - +1.3 140,511 - +0.5 140,511 - -0.8 140,528 + +0.2 140,535 + +0.4 140,535 + +0.1 140,605 - +1.2 140,632 - -0.8 140,644 + PP_0133 0.11 -3.1 140,737 + PP_0133 0.18 -0.6 140,738 - PP_0133 0.18 -0.2 140,774 + PP_0133 0.20 -0.3 140,829 - PP_0133 0.24 -0.8 140,930 + PP_0133 0.32 +1.3 141,000 + PP_0133 0.37 +0.1 141,000 + PP_0133 0.37 -0.2 141,001 - PP_0133 0.37 +0.5 141,001 - PP_0133 0.37 +2.3 141,004 + PP_0133 0.37 -1.3 141,004 + PP_0133 0.37 -0.2 141,004 + PP_0133 0.37 -0.5 141,004 + PP_0133 0.37 +2.9 141,004 + PP_0133 0.37 -1.1 141,004 + PP_0133 0.37 +0.6 141,005 - PP_0133 0.37 -0.1 141,005 - PP_0133 0.37 +1.2 141,005 - PP_0133 0.37 +0.7 141,005 - PP_0133 0.37 +1.6 141,005 - PP_0133 0.37 +1.5 141,045 - PP_0133 0.40 +0.6 141,045 - PP_0133 0.40 -0.1 141,119 - PP_0133 0.46 -0.3 141,199 + PP_0133 0.52 +1.0 141,200 - PP_0133 0.52 +1.7 141,200 - PP_0133 0.52 -0.8 141,396 + PP_0133 0.67 -0.1 141,396 + PP_0133 0.67 +0.6 141,397 - PP_0133 0.67 +0.1 141,427 + PP_0133 0.69 -0.3 141,428 - PP_0133 0.69 -3.8
Or see this region's nucleotide sequence