Experiment: P. putida Sci small MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2767 and PP_2768 overlap by 25 nucleotides PP_2768 and PP_2769 are separated by 12 nucleotides PP_2769 and PP_2770 are separated by 67 nucleotides
PP_2767: PP_2767 - putative Branched-chain amino acid ABC transporter, ATP-binding protein, at 3,151,037 to 3,151,870
_2767
PP_2768: PP_2768 - putative Branched-chain amino acid ABC transporter, permease protein, at 3,151,846 to 3,152,727
_2768
PP_2769: PP_2769 - putative Branched-chain amino acid ABC transporter, permease protein, at 3,152,740 to 3,153,729
_2769
PP_2770: PP_2770 - conserved exported protein of unknown function, at 3,153,797 to 3,155,122
_2770
Position (kb)
3152
3153
3154 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3151.897 kb on + strand at 3151.897 kb on + strand at 3151.897 kb on + strand at 3151.897 kb on + strand at 3151.898 kb on - strand at 3151.922 kb on + strand at 3151.931 kb on - strand at 3151.931 kb on - strand at 3151.974 kb on - strand, within PP_2768 at 3152.152 kb on + strand, within PP_2768 at 3152.153 kb on - strand, within PP_2768 at 3152.153 kb on - strand, within PP_2768 at 3152.153 kb on - strand, within PP_2768 at 3152.542 kb on - strand, within PP_2768 at 3152.609 kb on - strand, within PP_2768 at 3152.654 kb on - strand at 3152.909 kb on + strand, within PP_2769 at 3152.910 kb on - strand, within PP_2769 at 3152.910 kb on - strand, within PP_2769 at 3152.996 kb on + strand, within PP_2769 at 3153.013 kb on + strand, within PP_2769 at 3153.014 kb on - strand, within PP_2769 at 3153.015 kb on + strand, within PP_2769 at 3153.133 kb on + strand, within PP_2769 at 3153.134 kb on - strand, within PP_2769 at 3153.377 kb on - strand, within PP_2769 at 3153.412 kb on + strand, within PP_2769 at 3153.413 kb on - strand, within PP_2769 at 3153.481 kb on - strand, within PP_2769 at 3153.603 kb on - strand, within PP_2769 at 3153.603 kb on - strand, within PP_2769 at 3153.782 kb on + strand at 3153.788 kb on - strand at 3153.843 kb on + strand at 3153.843 kb on + strand at 3153.843 kb on + strand at 3153.844 kb on - strand at 3153.844 kb on - strand at 3153.885 kb on - strand at 3153.885 kb on - strand at 3153.918 kb on - strand at 3153.918 kb on - strand at 3153.918 kb on - strand at 3153.959 kb on + strand, within PP_2770 at 3153.968 kb on + strand, within PP_2770 at 3154.059 kb on - strand, within PP_2770 at 3154.130 kb on + strand, within PP_2770 at 3154.131 kb on - strand, within PP_2770 at 3154.223 kb on + strand, within PP_2770 at 3154.224 kb on - strand, within PP_2770 at 3154.224 kb on - strand, within PP_2770 at 3154.261 kb on - strand, within PP_2770 at 3154.398 kb on - strand, within PP_2770 at 3154.398 kb on - strand, within PP_2770
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Sci small MOI 0.1 remove 3,151,897 + +0.2 3,151,897 + -4.6 3,151,897 + +0.6 3,151,897 + +0.6 3,151,898 - -4.2 3,151,922 + -3.9 3,151,931 - -4.6 3,151,931 - +0.8 3,151,974 - PP_2768 0.15 -3.2 3,152,152 + PP_2768 0.35 -4.9 3,152,153 - PP_2768 0.35 +0.9 3,152,153 - PP_2768 0.35 +0.7 3,152,153 - PP_2768 0.35 +2.3 3,152,542 - PP_2768 0.79 -2.0 3,152,609 - PP_2768 0.87 -2.0 3,152,654 - +0.7 3,152,909 + PP_2769 0.17 -3.8 3,152,910 - PP_2769 0.17 +0.4 3,152,910 - PP_2769 0.17 -4.6 3,152,996 + PP_2769 0.26 +0.4 3,153,013 + PP_2769 0.28 -2.6 3,153,014 - PP_2769 0.28 -3.6 3,153,015 + PP_2769 0.28 -2.4 3,153,133 + PP_2769 0.40 -2.0 3,153,134 - PP_2769 0.40 -4.4 3,153,377 - PP_2769 0.64 -4.2 3,153,412 + PP_2769 0.68 +0.7 3,153,413 - PP_2769 0.68 +2.1 3,153,481 - PP_2769 0.75 +0.5 3,153,603 - PP_2769 0.87 -0.7 3,153,603 - PP_2769 0.87 -3.2 3,153,782 + -0.2 3,153,788 - -0.0 3,153,843 + +0.9 3,153,843 + +1.7 3,153,843 + +2.3 3,153,844 - +1.1 3,153,844 - +2.1 3,153,885 - -3.5 3,153,885 - -3.0 3,153,918 - -3.9 3,153,918 - +2.0 3,153,918 - -3.6 3,153,959 + PP_2770 0.12 -0.2 3,153,968 + PP_2770 0.13 +0.4 3,154,059 - PP_2770 0.20 -2.9 3,154,130 + PP_2770 0.25 +0.3 3,154,131 - PP_2770 0.25 -3.2 3,154,223 + PP_2770 0.32 +1.1 3,154,224 - PP_2770 0.32 -0.0 3,154,224 - PP_2770 0.32 -0.7 3,154,261 - PP_2770 0.35 -0.0 3,154,398 - PP_2770 0.45 -5.0 3,154,398 - PP_2770 0.45 -0.2
Or see this region's nucleotide sequence