Experiment: P. putida Sci small MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5285 and PP_5286 are separated by 5 nucleotides PP_5286 and PP_5288 are separated by 120 nucleotides PP_5288 and PP_5289 are separated by 30 nucleotides
PP_5285: PP_5285 - coenzyme A biosynthesis bifunctional protein (fused 4'-phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase), at 6,033,678 to 6,034,889
_5285
PP_5286: PP_5286 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 6,034,895 to 6,035,350
_5286
PP_5288: PP_5288 - Phosphomannomutase/phosphoglucomutase, at 6,035,471 to 6,036,862
_5288
PP_5289: PP_5289 - Acetylglutamate kinase, at 6,036,893 to 6,037,798
_5289
Position (kb)
6035
6036
6037 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6034.887 kb on + strand at 6034.892 kb on + strand at 6034.892 kb on + strand at 6035.519 kb on + strand at 6035.519 kb on + strand at 6035.519 kb on + strand at 6035.519 kb on + strand at 6035.519 kb on + strand at 6035.519 kb on + strand at 6035.519 kb on + strand at 6035.520 kb on - strand at 6035.520 kb on - strand at 6035.742 kb on - strand, within PP_5288 at 6035.744 kb on + strand, within PP_5288 at 6035.744 kb on + strand, within PP_5288 at 6035.744 kb on + strand, within PP_5288 at 6035.745 kb on - strand, within PP_5288 at 6035.745 kb on - strand, within PP_5288 at 6035.745 kb on - strand, within PP_5288 at 6035.745 kb on - strand, within PP_5288 at 6035.810 kb on + strand, within PP_5288 at 6035.811 kb on - strand at 6035.945 kb on + strand, within PP_5288 at 6035.946 kb on - strand, within PP_5288 at 6036.245 kb on + strand, within PP_5288 at 6036.500 kb on + strand, within PP_5288 at 6036.500 kb on + strand, within PP_5288 at 6036.500 kb on + strand at 6036.500 kb on + strand, within PP_5288 at 6036.500 kb on + strand, within PP_5288 at 6036.500 kb on + strand, within PP_5288 at 6036.500 kb on + strand, within PP_5288 at 6036.500 kb on + strand, within PP_5288 at 6036.501 kb on - strand, within PP_5288 at 6036.501 kb on - strand, within PP_5288 at 6036.501 kb on - strand at 6036.501 kb on - strand, within PP_5288 at 6036.501 kb on - strand, within PP_5288 at 6036.502 kb on + strand, within PP_5288 at 6036.601 kb on + strand, within PP_5288 at 6036.601 kb on + strand, within PP_5288 at 6036.602 kb on - strand, within PP_5288 at 6036.759 kb on + strand at 6036.760 kb on - strand at 6036.760 kb on - strand at 6036.760 kb on - strand at 6036.799 kb on + strand at 6036.799 kb on + strand at 6036.799 kb on + strand at 6036.800 kb on - strand at 6036.800 kb on - strand at 6036.800 kb on - strand at 6036.868 kb on + strand at 6036.868 kb on + strand at 6036.868 kb on + strand at 6036.869 kb on - strand at 6036.879 kb on + strand at 6036.880 kb on - strand at 6036.924 kb on + strand at 6036.925 kb on - strand at 6036.925 kb on - strand at 6036.925 kb on - strand at 6036.925 kb on - strand at 6036.953 kb on + strand at 6036.954 kb on - strand at 6036.954 kb on - strand at 6036.954 kb on - strand at 6036.954 kb on - strand at 6037.083 kb on + strand, within PP_5289 at 6037.084 kb on - strand, within PP_5289 at 6037.084 kb on - strand, within PP_5289 at 6037.472 kb on + strand, within PP_5289 at 6037.473 kb on - strand, within PP_5289 at 6037.637 kb on + strand, within PP_5289 at 6037.638 kb on - strand, within PP_5289 at 6037.638 kb on - strand, within PP_5289 at 6037.638 kb on - strand, within PP_5289 at 6037.777 kb on + strand at 6037.778 kb on - strand at 6037.833 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Sci small MOI 0.1 remove 6,034,887 + -0.2 6,034,892 + +0.8 6,034,892 + +0.8 6,035,519 + +1.1 6,035,519 + -1.0 6,035,519 + +2.2 6,035,519 + +1.0 6,035,519 + -1.1 6,035,519 + -3.8 6,035,519 + +1.0 6,035,520 - +1.6 6,035,520 - +0.5 6,035,742 - PP_5288 0.19 -1.5 6,035,744 + PP_5288 0.20 +0.5 6,035,744 + PP_5288 0.20 +2.2 6,035,744 + PP_5288 0.20 +1.0 6,035,745 - PP_5288 0.20 -2.5 6,035,745 - PP_5288 0.20 +0.8 6,035,745 - PP_5288 0.20 +1.2 6,035,745 - PP_5288 0.20 -3.3 6,035,810 + PP_5288 0.24 -0.6 6,035,811 - +0.8 6,035,945 + PP_5288 0.34 +0.0 6,035,946 - PP_5288 0.34 +2.1 6,036,245 + PP_5288 0.56 +1.6 6,036,500 + PP_5288 0.74 +2.2 6,036,500 + PP_5288 0.74 +0.7 6,036,500 + +1.8 6,036,500 + PP_5288 0.74 +1.8 6,036,500 + PP_5288 0.74 +1.3 6,036,500 + PP_5288 0.74 -0.4 6,036,500 + PP_5288 0.74 +0.5 6,036,500 + PP_5288 0.74 +1.0 6,036,501 - PP_5288 0.74 +0.1 6,036,501 - PP_5288 0.74 +0.6 6,036,501 - +1.2 6,036,501 - PP_5288 0.74 +1.7 6,036,501 - PP_5288 0.74 -2.6 6,036,502 + PP_5288 0.74 +1.6 6,036,601 + PP_5288 0.81 +0.6 6,036,601 + PP_5288 0.81 +0.8 6,036,602 - PP_5288 0.81 -3.4 6,036,759 + +1.3 6,036,760 - -0.4 6,036,760 - -0.4 6,036,760 - +2.0 6,036,799 + -4.3 6,036,799 + -1.5 6,036,799 + +1.6 6,036,800 - -3.4 6,036,800 - -4.1 6,036,800 - -1.6 6,036,868 + -1.8 6,036,868 + +1.4 6,036,868 + +0.1 6,036,869 - +1.6 6,036,879 + +1.2 6,036,880 - -3.9 6,036,924 + -3.6 6,036,925 - +1.0 6,036,925 - +0.8 6,036,925 - -2.5 6,036,925 - -2.6 6,036,953 + +0.0 6,036,954 - -3.2 6,036,954 - -0.7 6,036,954 - -0.3 6,036,954 - +0.1 6,037,083 + PP_5289 0.21 -3.2 6,037,084 - PP_5289 0.21 +3.3 6,037,084 - PP_5289 0.21 +0.5 6,037,472 + PP_5289 0.64 -3.8 6,037,473 - PP_5289 0.64 +1.6 6,037,637 + PP_5289 0.82 -1.4 6,037,638 - PP_5289 0.82 -4.0 6,037,638 - PP_5289 0.82 -4.0 6,037,638 - PP_5289 0.82 -3.9 6,037,777 + +1.8 6,037,778 - +1.0 6,037,833 + -3.8
Or see this region's nucleotide sequence