Experiment: P. putida Sci small MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3276 and PP_3277 are separated by 8 nucleotides PP_3277 and PP_3278 are separated by 25 nucleotides PP_3278 and PP_3279 are separated by 198 nucleotides
PP_3276: PP_3276 - ring 1,2-phenylacetyl-CoA epoxidase beta subunit, at 3,710,932 to 3,711,690
_3276
PP_3277: PP_3277 - ring 1,2-phenylacetyl-CoA epoxidase regulatory subunit, at 3,711,699 to 3,711,980
_3277
PP_3278: PP_3278 - ring 1,2-phenylacetyl-CoA epoxidase alpha subunit, at 3,712,006 to 3,712,995
_3278
PP_3279: PP_3279 - phenylacetate-CoA ligase, at 3,713,194 to 3,714,513
_3279
Position (kb)
3712
3713 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3711.029 kb on + strand, within PP_3276 at 3711.029 kb on + strand, within PP_3276 at 3711.287 kb on + strand, within PP_3276 at 3711.287 kb on + strand, within PP_3276 at 3711.288 kb on - strand, within PP_3276 at 3711.522 kb on - strand, within PP_3276 at 3711.522 kb on - strand, within PP_3276 at 3711.665 kb on + strand at 3711.665 kb on + strand at 3711.684 kb on + strand at 3711.684 kb on + strand at 3711.684 kb on + strand at 3711.684 kb on + strand at 3711.685 kb on - strand at 3711.685 kb on - strand at 3711.685 kb on - strand at 3711.731 kb on - strand, within PP_3277 at 3711.748 kb on + strand, within PP_3277 at 3711.749 kb on - strand, within PP_3277 at 3711.749 kb on - strand, within PP_3277 at 3711.849 kb on - strand, within PP_3277 at 3711.860 kb on - strand, within PP_3277 at 3711.860 kb on - strand, within PP_3277 at 3712.040 kb on + strand at 3712.040 kb on + strand at 3712.041 kb on - strand at 3712.142 kb on + strand, within PP_3278 at 3712.176 kb on - strand, within PP_3278 at 3712.489 kb on - strand, within PP_3278 at 3712.534 kb on + strand, within PP_3278 at 3712.535 kb on - strand, within PP_3278 at 3712.610 kb on + strand, within PP_3278 at 3712.610 kb on + strand, within PP_3278 at 3712.611 kb on - strand, within PP_3278 at 3712.628 kb on + strand, within PP_3278 at 3712.700 kb on + strand, within PP_3278 at 3712.706 kb on + strand, within PP_3278 at 3712.992 kb on - strand at 3713.012 kb on + strand at 3713.086 kb on + strand at 3713.294 kb on + strand at 3713.294 kb on + strand at 3713.294 kb on + strand at 3713.295 kb on - strand at 3713.638 kb on + strand, within PP_3279 at 3713.638 kb on + strand, within PP_3279 at 3713.702 kb on + strand, within PP_3279 at 3713.702 kb on + strand, within PP_3279 at 3713.743 kb on + strand, within PP_3279 at 3713.743 kb on + strand, within PP_3279 at 3713.744 kb on - strand, within PP_3279 at 3713.790 kb on - strand, within PP_3279 at 3713.790 kb on - strand, within PP_3279 at 3713.794 kb on - strand, within PP_3279 at 3713.939 kb on + strand, within PP_3279 at 3713.939 kb on + strand, within PP_3279 at 3713.940 kb on - strand, within PP_3279 at 3713.940 kb on - strand, within PP_3279 at 3713.940 kb on - strand, within PP_3279
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Sci small MOI 0.1 remove 3,711,029 + PP_3276 0.13 -0.6 3,711,029 + PP_3276 0.13 +0.8 3,711,287 + PP_3276 0.47 +1.6 3,711,287 + PP_3276 0.47 +0.1 3,711,288 - PP_3276 0.47 +0.7 3,711,522 - PP_3276 0.78 -2.7 3,711,522 - PP_3276 0.78 -3.7 3,711,665 + +0.1 3,711,665 + -2.6 3,711,684 + +0.8 3,711,684 + -0.2 3,711,684 + -4.0 3,711,684 + +2.0 3,711,685 - +1.9 3,711,685 - -3.2 3,711,685 - +1.3 3,711,731 - PP_3277 0.11 +1.5 3,711,748 + PP_3277 0.17 -3.0 3,711,749 - PP_3277 0.18 -0.5 3,711,749 - PP_3277 0.18 -2.5 3,711,849 - PP_3277 0.53 +1.8 3,711,860 - PP_3277 0.57 +0.9 3,711,860 - PP_3277 0.57 -3.5 3,712,040 + -2.9 3,712,040 + +1.1 3,712,041 - -0.7 3,712,142 + PP_3278 0.14 +0.6 3,712,176 - PP_3278 0.17 -3.6 3,712,489 - PP_3278 0.49 -1.0 3,712,534 + PP_3278 0.53 +1.0 3,712,535 - PP_3278 0.53 -3.2 3,712,610 + PP_3278 0.61 +1.3 3,712,610 + PP_3278 0.61 +2.3 3,712,611 - PP_3278 0.61 +1.5 3,712,628 + PP_3278 0.63 -0.1 3,712,700 + PP_3278 0.70 +1.4 3,712,706 + PP_3278 0.71 -3.0 3,712,992 - +2.1 3,713,012 + +2.2 3,713,086 + -3.5 3,713,294 + -3.6 3,713,294 + -3.9 3,713,294 + -4.0 3,713,295 - -2.9 3,713,638 + PP_3279 0.34 +0.9 3,713,638 + PP_3279 0.34 +0.4 3,713,702 + PP_3279 0.38 +0.5 3,713,702 + PP_3279 0.38 -3.2 3,713,743 + PP_3279 0.42 -3.4 3,713,743 + PP_3279 0.42 -2.7 3,713,744 - PP_3279 0.42 -0.3 3,713,790 - PP_3279 0.45 +0.4 3,713,790 - PP_3279 0.45 +0.8 3,713,794 - PP_3279 0.45 -3.0 3,713,939 + PP_3279 0.56 -4.3 3,713,939 + PP_3279 0.56 -2.6 3,713,940 - PP_3279 0.57 -4.1 3,713,940 - PP_3279 0.57 -3.3 3,713,940 - PP_3279 0.57 -0.7
Or see this region's nucleotide sequence