Experiment: P. putida Sci small MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3032 and PP_5552 are separated by 3 nucleotides PP_5552 and PP_5553 overlap by 4 nucleotides PP_5553 and PP_3033 are separated by 107 nucleotides PP_3033 and PP_3034 are separated by 78 nucleotides PP_3034 and PP_3035 overlap by 90 nucleotides PP_3035 and PP_5554 are separated by 108 nucleotides PP_5554 and PP_3036 are separated by 71 nucleotides PP_3036 and PP_5555 overlap by 29 nucleotides
PP_3032: PP_3032 - DNA-binding protein Roi-related protein, at 3,418,570 to 3,418,860
_3032
PP_5552: PP_5552 - conserved protein of unknown function, at 3,418,864 to 3,419,175
_5552
PP_5553: PP_5553 - conserved protein of unknown function, at 3,419,172 to 3,419,345
_5553
PP_3033: PP_3033 - putative transcriptional repressor, CI/C2 family, at 3,419,453 to 3,420,184
_3033
PP_3034: PP_3034 - putative Regulator protein, at 3,420,263 to 3,420,526
_3034
PP_3035: PP_3035 - conserved protein of unknown function, at 3,420,437 to 3,420,610
_3035
PP_5554: PP_5554 - protein of unknown function, at 3,420,719 to 3,420,901
_5554
PP_3036: PP_3036 - conserved protein of unknown function, at 3,420,973 to 3,421,152
_3036
PP_5555: PP_5555 - conserved protein of unknown function, at 3,421,124 to 3,421,642
_5555
Position (kb)
3419
3420
3421 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3418.580 kb on + strand at 3418.673 kb on + strand, within PP_3032 at 3418.865 kb on + strand at 3418.880 kb on - strand at 3418.880 kb on - strand at 3418.882 kb on + strand at 3418.883 kb on - strand at 3418.883 kb on - strand at 3418.932 kb on + strand, within PP_5552 at 3419.137 kb on - strand, within PP_5552 at 3419.168 kb on + strand at 3419.195 kb on - strand, within PP_5553 at 3419.195 kb on - strand, within PP_5553 at 3419.228 kb on + strand, within PP_5553 at 3419.229 kb on - strand, within PP_5553 at 3419.241 kb on - strand, within PP_5553 at 3419.241 kb on - strand, within PP_5553 at 3419.241 kb on - strand, within PP_5553 at 3419.292 kb on + strand, within PP_5553 at 3419.325 kb on + strand, within PP_5553 at 3419.325 kb on + strand, within PP_5553 at 3419.325 kb on + strand, within PP_5553 at 3419.325 kb on + strand at 3419.325 kb on + strand, within PP_5553 at 3419.326 kb on - strand, within PP_5553 at 3419.326 kb on - strand at 3419.326 kb on - strand, within PP_5553 at 3419.326 kb on - strand, within PP_5553 at 3419.326 kb on - strand, within PP_5553 at 3419.348 kb on + strand at 3419.348 kb on + strand at 3420.352 kb on - strand, within PP_3034 at 3420.357 kb on - strand at 3420.357 kb on - strand, within PP_3034 at 3420.398 kb on - strand, within PP_3034 at 3420.408 kb on - strand, within PP_3034 at 3420.525 kb on - strand at 3420.565 kb on - strand, within PP_3035 at 3420.565 kb on - strand, within PP_3035 at 3420.727 kb on - strand at 3420.730 kb on - strand at 3420.730 kb on - strand at 3420.758 kb on - strand, within PP_5554 at 3420.791 kb on - strand, within PP_5554 at 3420.791 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.800 kb on - strand, within PP_5554 at 3420.881 kb on - strand, within PP_5554 at 3420.885 kb on - strand at 3420.902 kb on - strand at 3420.902 kb on - strand at 3420.902 kb on - strand at 3420.902 kb on - strand at 3420.902 kb on - strand at 3420.933 kb on + strand at 3420.977 kb on - strand at 3421.054 kb on + strand, within PP_3036 at 3421.055 kb on - strand, within PP_3036 at 3421.055 kb on - strand, within PP_3036 at 3421.055 kb on - strand, within PP_3036 at 3421.055 kb on - strand, within PP_3036 at 3421.062 kb on + strand, within PP_3036 at 3421.063 kb on - strand, within PP_3036
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Sci small MOI 0.1 remove 3,418,580 + -4.0 3,418,673 + PP_3032 0.35 -1.6 3,418,865 + +0.8 3,418,880 - -2.5 3,418,880 - -0.3 3,418,882 + +0.3 3,418,883 - +1.1 3,418,883 - -2.5 3,418,932 + PP_5552 0.22 +1.9 3,419,137 - PP_5552 0.88 +1.1 3,419,168 + +0.2 3,419,195 - PP_5553 0.13 +0.3 3,419,195 - PP_5553 0.13 -2.7 3,419,228 + PP_5553 0.32 -4.0 3,419,229 - PP_5553 0.33 +1.2 3,419,241 - PP_5553 0.40 -0.8 3,419,241 - PP_5553 0.40 +1.6 3,419,241 - PP_5553 0.40 +0.3 3,419,292 + PP_5553 0.69 -3.2 3,419,325 + PP_5553 0.88 -3.2 3,419,325 + PP_5553 0.88 +1.8 3,419,325 + PP_5553 0.88 +0.2 3,419,325 + -1.9 3,419,325 + PP_5553 0.88 -2.2 3,419,326 - PP_5553 0.89 -3.3 3,419,326 - +0.7 3,419,326 - PP_5553 0.89 +0.7 3,419,326 - PP_5553 0.89 -0.9 3,419,326 - PP_5553 0.89 -2.8 3,419,348 + +0.7 3,419,348 + +1.3 3,420,352 - PP_3034 0.34 +1.4 3,420,357 - +2.7 3,420,357 - PP_3034 0.36 -2.5 3,420,398 - PP_3034 0.51 -0.6 3,420,408 - PP_3034 0.55 -3.0 3,420,525 - -3.1 3,420,565 - PP_3035 0.74 -3.7 3,420,565 - PP_3035 0.74 -4.6 3,420,727 - +0.5 3,420,730 - -0.2 3,420,730 - -2.5 3,420,758 - PP_5554 0.21 -3.4 3,420,791 - PP_5554 0.39 +1.0 3,420,791 - PP_5554 0.39 -2.8 3,420,800 - PP_5554 0.44 -2.8 3,420,800 - PP_5554 0.44 +0.4 3,420,800 - PP_5554 0.44 -3.2 3,420,800 - PP_5554 0.44 -3.5 3,420,800 - PP_5554 0.44 +1.7 3,420,800 - PP_5554 0.44 +0.7 3,420,800 - PP_5554 0.44 -2.9 3,420,800 - PP_5554 0.44 -1.1 3,420,881 - PP_5554 0.89 -2.7 3,420,885 - +1.9 3,420,902 - -3.0 3,420,902 - -3.7 3,420,902 - +1.7 3,420,902 - +1.8 3,420,902 - +1.1 3,420,933 + -3.3 3,420,977 - -3.2 3,421,054 + PP_3036 0.45 -0.5 3,421,055 - PP_3036 0.46 +1.1 3,421,055 - PP_3036 0.46 -0.7 3,421,055 - PP_3036 0.46 -0.9 3,421,055 - PP_3036 0.46 -3.4 3,421,062 + PP_3036 0.49 -4.0 3,421,063 - PP_3036 0.50 -3.0
Or see this region's nucleotide sequence