Experiment: P. putida Sci small MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0660 and PP_0661 are separated by 45 nucleotides PP_0661 and PP_0662 are separated by 24 nucleotides PP_0662 and PP_0663 overlap by 4 nucleotides PP_0663 and PP_0664 are separated by 198 nucleotides
PP_0660: PP_0660 - S-methyl-L-methionine transporter, at 767,923 to 769,362
_0660
PP_0661: PP_0661 - putative Transcriptional regulator AmpR, at 769,408 to 770,292
_0661
PP_0662: PP_0662 - putative Threonine synthase, at 770,317 to 771,705
_0662
PP_0663: PP_0663 - Transcriptional regulator, AsnC family, at 771,702 to 772,139
_0663
PP_0664: PP_0664 - homoserine dehydrogenase, at 772,338 to 773,381
_0664
Position (kb) 770
771
772 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 769.372 kb on - strand at 769.372 kb on - strand at 769.372 kb on - strand at 769.374 kb on + strand at 769.374 kb on + strand at 769.374 kb on + strand at 769.374 kb on + strand at 769.374 kb on + strand at 769.506 kb on + strand, within PP_0661 at 769.506 kb on + strand, within PP_0661 at 769.615 kb on + strand, within PP_0661 at 769.615 kb on + strand, within PP_0661 at 769.615 kb on + strand, within PP_0661 at 769.616 kb on - strand, within PP_0661 at 769.638 kb on + strand, within PP_0661 at 769.703 kb on + strand, within PP_0661 at 769.789 kb on + strand, within PP_0661 at 769.790 kb on - strand, within PP_0661 at 769.790 kb on - strand, within PP_0661 at 770.012 kb on - strand, within PP_0661 at 770.012 kb on - strand, within PP_0661 at 770.138 kb on + strand, within PP_0661 at 770.290 kb on + strand at 770.291 kb on - strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.295 kb on + strand at 770.296 kb on - strand at 770.296 kb on - strand at 770.296 kb on - strand at 770.299 kb on + strand at 770.299 kb on + strand at 770.299 kb on + strand at 770.299 kb on + strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.300 kb on - strand at 770.303 kb on + strand at 770.304 kb on - strand at 770.304 kb on - strand at 770.304 kb on - strand at 770.323 kb on + strand at 770.324 kb on - strand at 770.324 kb on - strand at 770.324 kb on - strand at 770.324 kb on - strand at 770.404 kb on + strand at 770.404 kb on + strand at 770.526 kb on + strand, within PP_0662 at 770.555 kb on - strand, within PP_0662 at 770.555 kb on - strand, within PP_0662 at 770.805 kb on - strand, within PP_0662 at 770.805 kb on - strand, within PP_0662 at 770.806 kb on + strand, within PP_0662 at 770.807 kb on - strand, within PP_0662 at 770.807 kb on - strand, within PP_0662 at 771.014 kb on - strand, within PP_0662 at 771.014 kb on - strand, within PP_0662 at 771.014 kb on - strand, within PP_0662 at 771.014 kb on - strand, within PP_0662 at 771.014 kb on - strand, within PP_0662 at 771.094 kb on + strand, within PP_0662 at 771.094 kb on + strand, within PP_0662 at 771.095 kb on - strand, within PP_0662 at 771.095 kb on - strand, within PP_0662 at 771.095 kb on - strand, within PP_0662 at 771.196 kb on + strand, within PP_0662 at 771.283 kb on + strand, within PP_0662 at 771.283 kb on + strand, within PP_0662 at 771.284 kb on - strand, within PP_0662 at 771.284 kb on - strand, within PP_0662 at 771.284 kb on - strand, within PP_0662 at 771.394 kb on + strand, within PP_0662 at 771.395 kb on - strand, within PP_0662 at 771.461 kb on + strand, within PP_0662 at 771.485 kb on + strand, within PP_0662 at 771.486 kb on - strand, within PP_0662 at 771.863 kb on + strand, within PP_0663 at 771.863 kb on + strand, within PP_0663 at 771.864 kb on - strand, within PP_0663 at 771.864 kb on - strand, within PP_0663 at 771.864 kb on - strand, within PP_0663 at 771.924 kb on + strand, within PP_0663 at 771.924 kb on + strand, within PP_0663 at 771.931 kb on - strand, within PP_0663 at 772.159 kb on + strand at 772.249 kb on + strand at 772.250 kb on - strand at 772.254 kb on - strand at 772.254 kb on - strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.293 kb on + strand at 772.294 kb on - strand at 772.298 kb on + strand at 772.299 kb on - strand at 772.299 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Sci small MOI 0.1 remove 769,372 - +0.8 769,372 - -3.7 769,372 - +0.2 769,374 + -4.5 769,374 + -3.6 769,374 + -0.5 769,374 + +0.4 769,374 + +1.0 769,506 + PP_0661 0.11 +1.3 769,506 + PP_0661 0.11 -3.7 769,615 + PP_0661 0.23 -0.4 769,615 + PP_0661 0.23 +1.6 769,615 + PP_0661 0.23 -4.0 769,616 - PP_0661 0.24 +1.2 769,638 + PP_0661 0.26 -3.7 769,703 + PP_0661 0.33 +0.4 769,789 + PP_0661 0.43 -3.3 769,790 - PP_0661 0.43 -0.8 769,790 - PP_0661 0.43 -2.2 770,012 - PP_0661 0.68 -4.1 770,012 - PP_0661 0.68 +0.3 770,138 + PP_0661 0.82 -0.6 770,290 + +0.2 770,291 - -4.5 770,295 + +0.2 770,295 + -3.4 770,295 + -2.7 770,295 + -0.6 770,295 + +2.0 770,295 + +0.6 770,295 + +1.2 770,295 + -2.4 770,296 - -3.7 770,296 - -4.3 770,296 - -0.4 770,299 + -4.4 770,299 + +0.7 770,299 + -0.2 770,299 + -2.9 770,300 - +1.2 770,300 - -2.4 770,300 - -4.6 770,300 - +0.5 770,300 - -0.9 770,300 - -4.3 770,300 - -3.3 770,300 - +0.6 770,303 + -2.9 770,304 - +1.8 770,304 - -3.0 770,304 - -4.3 770,323 + -4.2 770,324 - -4.2 770,324 - +0.9 770,324 - +1.5 770,324 - -0.9 770,404 + -5.0 770,404 + -4.9 770,526 + PP_0662 0.15 -3.4 770,555 - PP_0662 0.17 -4.1 770,555 - PP_0662 0.17 +0.8 770,805 - PP_0662 0.35 +0.8 770,805 - PP_0662 0.35 -3.5 770,806 + PP_0662 0.35 -3.6 770,807 - PP_0662 0.35 -3.2 770,807 - PP_0662 0.35 +0.2 771,014 - PP_0662 0.50 +0.7 771,014 - PP_0662 0.50 -2.3 771,014 - PP_0662 0.50 -0.1 771,014 - PP_0662 0.50 -3.5 771,014 - PP_0662 0.50 -3.7 771,094 + PP_0662 0.56 -3.2 771,094 + PP_0662 0.56 +2.7 771,095 - PP_0662 0.56 +0.6 771,095 - PP_0662 0.56 -0.5 771,095 - PP_0662 0.56 +2.5 771,196 + PP_0662 0.63 -3.9 771,283 + PP_0662 0.70 -4.8 771,283 + PP_0662 0.70 +0.0 771,284 - PP_0662 0.70 -1.9 771,284 - PP_0662 0.70 -0.2 771,284 - PP_0662 0.70 -4.0 771,394 + PP_0662 0.78 -3.2 771,395 - PP_0662 0.78 -3.7 771,461 + PP_0662 0.82 +1.9 771,485 + PP_0662 0.84 +1.0 771,486 - PP_0662 0.84 +1.1 771,863 + PP_0663 0.37 +1.1 771,863 + PP_0663 0.37 -1.2 771,864 - PP_0663 0.37 -0.2 771,864 - PP_0663 0.37 +0.4 771,864 - PP_0663 0.37 +0.1 771,924 + PP_0663 0.51 +2.9 771,924 + PP_0663 0.51 -3.3 771,931 - PP_0663 0.52 -0.5 772,159 + +1.3 772,249 + -2.2 772,250 - +2.7 772,254 - -1.2 772,254 - +0.3 772,293 + +0.6 772,293 + -3.4 772,293 + -4.0 772,293 + -0.5 772,293 + +0.9 772,293 + -2.2 772,294 - -0.9 772,298 + -0.5 772,299 - -2.4 772,299 - +1.1
Or see this region's nucleotide sequence