Experiment: P. putida Sci small MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0646 and PP_0647 overlap by 8 nucleotides PP_0647 and PP_0648 are separated by 191 nucleotides PP_0648 and PP_0649 are separated by 87 nucleotides PP_0649 and PP_0650 are separated by 27 nucleotides PP_0650 and PP_0651 are separated by 108 nucleotides
PP_0646: PP_0646 - conserved protein of unknown function, at 755,405 to 756,805
_0646
PP_0647: PP_0647 - conserved membrane protein of unknown function, at 756,798 to 757,187
_0647
PP_0648: PP_0648 - conserved protein of unknown function, at 757,379 to 757,834
_0648
PP_0649: PP_0649 - MutT/nudix family protein, at 757,922 to 758,332
_0649
PP_0650: PP_0650 - conserved protein of unknown function, at 758,360 to 758,470
_0650
PP_0651: PP_0651 - Acetyltransferase, GNAT family, at 758,579 to 759,028
_0651
Position (kb) 757
758 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 756.431 kb on + strand, within PP_0646 at 756.431 kb on + strand, within PP_0646 at 756.431 kb on + strand, within PP_0646 at 756.431 kb on + strand, within PP_0646 at 756.432 kb on - strand, within PP_0646 at 756.432 kb on - strand, within PP_0646 at 756.432 kb on - strand, within PP_0646 at 756.432 kb on - strand, within PP_0646 at 756.432 kb on - strand, within PP_0646 at 756.454 kb on + strand, within PP_0646 at 756.455 kb on - strand, within PP_0646 at 756.457 kb on + strand, within PP_0646 at 756.457 kb on + strand, within PP_0646 at 756.457 kb on + strand, within PP_0646 at 756.457 kb on + strand, within PP_0646 at 756.458 kb on - strand, within PP_0646 at 756.458 kb on - strand, within PP_0646 at 756.458 kb on - strand, within PP_0646 at 756.458 kb on - strand, within PP_0646 at 756.458 kb on - strand, within PP_0646 at 756.458 kb on - strand, within PP_0646 at 756.461 kb on + strand, within PP_0646 at 756.461 kb on + strand, within PP_0646 at 756.462 kb on - strand, within PP_0646 at 756.462 kb on - strand, within PP_0646 at 756.495 kb on + strand, within PP_0646 at 756.502 kb on + strand, within PP_0646 at 756.502 kb on + strand, within PP_0646 at 756.502 kb on + strand, within PP_0646 at 756.502 kb on + strand, within PP_0646 at 756.503 kb on - strand, within PP_0646 at 756.503 kb on - strand, within PP_0646 at 756.503 kb on - strand, within PP_0646 at 756.512 kb on + strand, within PP_0646 at 756.512 kb on + strand, within PP_0646 at 756.654 kb on + strand, within PP_0646 at 756.654 kb on + strand, within PP_0646 at 756.654 kb on + strand, within PP_0646 at 756.654 kb on + strand, within PP_0646 at 756.655 kb on - strand, within PP_0646 at 756.655 kb on - strand, within PP_0646 at 756.655 kb on - strand, within PP_0646 at 756.655 kb on - strand, within PP_0646 at 756.655 kb on - strand, within PP_0646 at 756.677 kb on - strand at 756.699 kb on + strand at 756.699 kb on + strand at 756.700 kb on - strand at 756.700 kb on - strand at 756.785 kb on + strand at 756.785 kb on + strand at 756.785 kb on + strand at 756.786 kb on - strand at 756.797 kb on + strand at 756.797 kb on + strand at 756.797 kb on + strand at 757.027 kb on + strand, within PP_0647 at 757.045 kb on + strand, within PP_0647 at 757.045 kb on + strand, within PP_0647 at 757.045 kb on + strand, within PP_0647 at 757.045 kb on + strand, within PP_0647 at 757.045 kb on + strand, within PP_0647 at 757.045 kb on + strand, within PP_0647 at 757.045 kb on + strand, within PP_0647 at 757.045 kb on + strand, within PP_0647 at 757.046 kb on - strand, within PP_0647 at 757.046 kb on - strand, within PP_0647 at 757.049 kb on + strand, within PP_0647 at 757.049 kb on + strand, within PP_0647 at 757.049 kb on + strand, within PP_0647 at 757.050 kb on - strand, within PP_0647 at 757.050 kb on - strand, within PP_0647 at 757.050 kb on - strand, within PP_0647 at 757.050 kb on - strand, within PP_0647 at 757.114 kb on + strand, within PP_0647 at 757.120 kb on + strand, within PP_0647 at 757.121 kb on - strand, within PP_0647 at 757.167 kb on + strand at 757.167 kb on + strand at 757.167 kb on + strand at 757.167 kb on + strand at 757.167 kb on + strand at 757.167 kb on + strand at 757.168 kb on - strand at 757.168 kb on - strand at 757.168 kb on - strand at 757.168 kb on - strand at 757.172 kb on - strand at 757.172 kb on - strand at 757.172 kb on - strand at 757.209 kb on + strand at 757.209 kb on + strand at 757.209 kb on + strand at 757.210 kb on - strand at 757.210 kb on - strand at 757.261 kb on - strand at 757.261 kb on - strand at 757.301 kb on - strand at 757.301 kb on - strand at 757.301 kb on - strand at 757.311 kb on + strand at 757.316 kb on + strand at 757.317 kb on - strand at 757.320 kb on + strand at 757.320 kb on + strand at 757.321 kb on - strand at 757.379 kb on - strand at 757.412 kb on + strand at 757.440 kb on + strand, within PP_0648 at 757.539 kb on + strand, within PP_0648 at 757.540 kb on - strand, within PP_0648 at 757.594 kb on + strand, within PP_0648 at 757.595 kb on - strand, within PP_0648 at 757.606 kb on + strand, within PP_0648 at 757.606 kb on + strand, within PP_0648 at 757.607 kb on - strand, within PP_0648 at 757.607 kb on - strand, within PP_0648 at 757.607 kb on - strand, within PP_0648 at 757.607 kb on - strand, within PP_0648 at 757.614 kb on - strand, within PP_0648 at 757.614 kb on - strand, within PP_0648 at 757.614 kb on - strand, within PP_0648 at 757.633 kb on + strand, within PP_0648 at 757.634 kb on - strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.639 kb on + strand, within PP_0648 at 757.639 kb on + strand, within PP_0648 at 757.640 kb on - strand, within PP_0648 at 757.640 kb on - strand, within PP_0648 at 757.641 kb on - strand, within PP_0648 at 757.673 kb on + strand, within PP_0648 at 757.674 kb on - strand, within PP_0648 at 757.674 kb on - strand, within PP_0648 at 757.693 kb on + strand, within PP_0648 at 757.756 kb on + strand, within PP_0648 at 757.756 kb on + strand, within PP_0648 at 757.757 kb on - strand, within PP_0648 at 757.757 kb on - strand, within PP_0648 at 757.767 kb on + strand, within PP_0648 at 757.767 kb on + strand, within PP_0648 at 757.803 kb on - strand at 757.838 kb on + strand at 757.880 kb on + strand at 757.880 kb on + strand at 757.880 kb on + strand at 757.900 kb on + strand at 757.900 kb on + strand at 757.900 kb on + strand at 757.947 kb on + strand at 757.947 kb on + strand at 757.948 kb on - strand at 757.948 kb on - strand at 757.948 kb on - strand at 757.948 kb on - strand at 757.948 kb on - strand at 757.980 kb on + strand, within PP_0649 at 757.998 kb on + strand, within PP_0649 at 757.998 kb on + strand, within PP_0649 at 757.999 kb on - strand, within PP_0649 at 758.087 kb on - strand, within PP_0649 at 758.087 kb on - strand, within PP_0649 at 758.189 kb on - strand, within PP_0649 at 758.189 kb on - strand, within PP_0649 at 758.306 kb on - strand at 758.330 kb on + strand at 758.331 kb on - strand at 758.331 kb on - strand at 758.331 kb on - strand at 758.331 kb on - strand at 758.340 kb on + strand at 758.340 kb on + strand at 758.341 kb on - strand at 758.341 kb on - strand at 758.358 kb on - strand at 758.384 kb on + strand, within PP_0650 at 758.384 kb on + strand, within PP_0650 at 758.385 kb on - strand, within PP_0650 at 758.385 kb on - strand, within PP_0650 at 758.387 kb on + strand, within PP_0650 at 758.387 kb on + strand, within PP_0650 at 758.388 kb on - strand, within PP_0650 at 758.388 kb on - strand, within PP_0650 at 758.388 kb on - strand, within PP_0650 at 758.388 kb on - strand, within PP_0650 at 758.446 kb on - strand, within PP_0650 at 758.446 kb on - strand, within PP_0650 at 758.451 kb on + strand, within PP_0650 at 758.451 kb on + strand, within PP_0650 at 758.451 kb on + strand, within PP_0650 at 758.452 kb on - strand, within PP_0650 at 758.452 kb on - strand, within PP_0650 at 758.486 kb on + strand at 758.486 kb on + strand at 758.486 kb on + strand at 758.514 kb on + strand at 758.514 kb on + strand at 758.515 kb on - strand at 758.538 kb on + strand at 758.539 kb on - strand at 758.539 kb on - strand at 758.539 kb on - strand at 758.560 kb on - strand at 758.562 kb on - strand at 758.626 kb on - strand, within PP_0651 at 758.626 kb on - strand, within PP_0651 at 758.638 kb on + strand, within PP_0651 at 758.638 kb on + strand, within PP_0651 at 758.639 kb on - strand, within PP_0651 at 758.640 kb on + strand, within PP_0651 at 758.640 kb on + strand, within PP_0651 at 758.737 kb on + strand, within PP_0651 at 758.738 kb on - strand at 758.757 kb on + strand, within PP_0651 at 758.757 kb on + strand, within PP_0651 at 758.758 kb on - strand, within PP_0651 at 758.758 kb on - strand, within PP_0651 at 758.763 kb on + strand, within PP_0651 at 758.764 kb on - strand, within PP_0651
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Sci small MOI 0.1 remove 756,431 + PP_0646 0.73 +1.5 756,431 + PP_0646 0.73 -0.6 756,431 + PP_0646 0.73 -0.0 756,431 + PP_0646 0.73 +2.1 756,432 - PP_0646 0.73 -0.5 756,432 - PP_0646 0.73 +0.8 756,432 - PP_0646 0.73 +1.6 756,432 - PP_0646 0.73 -1.3 756,432 - PP_0646 0.73 +0.3 756,454 + PP_0646 0.75 +0.3 756,455 - PP_0646 0.75 +1.4 756,457 + PP_0646 0.75 -0.2 756,457 + PP_0646 0.75 -1.1 756,457 + PP_0646 0.75 +0.9 756,457 + PP_0646 0.75 +0.8 756,458 - PP_0646 0.75 +0.9 756,458 - PP_0646 0.75 +1.5 756,458 - PP_0646 0.75 +0.2 756,458 - PP_0646 0.75 +2.3 756,458 - PP_0646 0.75 -2.4 756,458 - PP_0646 0.75 +0.7 756,461 + PP_0646 0.75 +2.3 756,461 + PP_0646 0.75 -3.3 756,462 - PP_0646 0.75 +1.4 756,462 - PP_0646 0.75 -3.4 756,495 + PP_0646 0.78 -3.1 756,502 + PP_0646 0.78 +1.4 756,502 + PP_0646 0.78 +0.1 756,502 + PP_0646 0.78 -3.5 756,502 + PP_0646 0.78 +0.4 756,503 - PP_0646 0.78 +2.5 756,503 - PP_0646 0.78 +0.1 756,503 - PP_0646 0.78 +2.2 756,512 + PP_0646 0.79 +1.7 756,512 + PP_0646 0.79 +0.7 756,654 + PP_0646 0.89 +0.7 756,654 + PP_0646 0.89 -4.9 756,654 + PP_0646 0.89 +2.3 756,654 + PP_0646 0.89 +0.1 756,655 - PP_0646 0.89 +0.7 756,655 - PP_0646 0.89 +2.0 756,655 - PP_0646 0.89 +1.3 756,655 - PP_0646 0.89 -3.2 756,655 - PP_0646 0.89 -1.0 756,677 - -1.2 756,699 + +0.7 756,699 + -3.1 756,700 - -0.6 756,700 - -3.8 756,785 + +2.2 756,785 + -0.1 756,785 + -3.8 756,786 - +1.3 756,797 + +0.3 756,797 + -3.8 756,797 + -1.8 757,027 + PP_0647 0.59 -0.3 757,045 + PP_0647 0.63 +0.8 757,045 + PP_0647 0.63 -0.8 757,045 + PP_0647 0.63 -0.2 757,045 + PP_0647 0.63 -3.4 757,045 + PP_0647 0.63 -0.0 757,045 + PP_0647 0.63 +0.7 757,045 + PP_0647 0.63 +1.5 757,045 + PP_0647 0.63 +0.4 757,046 - PP_0647 0.64 -0.0 757,046 - PP_0647 0.64 +1.4 757,049 + PP_0647 0.64 +0.2 757,049 + PP_0647 0.64 +1.4 757,049 + PP_0647 0.64 +1.5 757,050 - PP_0647 0.65 +1.0 757,050 - PP_0647 0.65 -0.8 757,050 - PP_0647 0.65 -2.4 757,050 - PP_0647 0.65 -3.7 757,114 + PP_0647 0.81 +0.1 757,120 + PP_0647 0.83 -0.3 757,121 - PP_0647 0.83 +2.0 757,167 + +1.0 757,167 + -4.2 757,167 + -0.8 757,167 + +0.0 757,167 + +1.7 757,167 + -3.6 757,168 - +0.4 757,168 - -3.2 757,168 - -3.7 757,168 - +1.5 757,172 - +2.2 757,172 - +0.5 757,172 - -3.6 757,209 + +0.4 757,209 + +2.0 757,209 + +0.1 757,210 - +1.5 757,210 - -3.9 757,261 - +0.3 757,261 - -3.9 757,301 - +0.5 757,301 - -0.2 757,301 - +1.2 757,311 + +0.3 757,316 + +1.4 757,317 - +0.0 757,320 + +1.4 757,320 + +0.9 757,321 - +2.3 757,379 - +1.4 757,412 + -4.1 757,440 + PP_0648 0.13 +1.0 757,539 + PP_0648 0.35 -4.2 757,540 - PP_0648 0.35 +0.8 757,594 + PP_0648 0.47 -2.4 757,595 - PP_0648 0.47 -3.1 757,606 + PP_0648 0.50 +0.4 757,606 + PP_0648 0.50 -1.3 757,607 - PP_0648 0.50 -4.3 757,607 - PP_0648 0.50 -4.0 757,607 - PP_0648 0.50 +1.3 757,607 - PP_0648 0.50 +0.9 757,614 - PP_0648 0.52 +0.6 757,614 - PP_0648 0.52 +0.6 757,614 - PP_0648 0.52 +1.2 757,633 + PP_0648 0.56 +0.7 757,634 - PP_0648 0.56 -0.3 757,637 + PP_0648 0.57 +1.2 757,637 + PP_0648 0.57 +1.1 757,637 + PP_0648 0.57 -1.2 757,637 + PP_0648 0.57 +0.1 757,637 + PP_0648 0.57 -3.5 757,637 + PP_0648 0.57 +1.7 757,637 + PP_0648 0.57 -2.4 757,638 - PP_0648 0.57 -2.8 757,638 - PP_0648 0.57 +1.2 757,638 - PP_0648 0.57 +2.3 757,638 - PP_0648 0.57 +1.2 757,638 - PP_0648 0.57 +1.0 757,638 - PP_0648 0.57 -2.2 757,638 - PP_0648 0.57 +1.4 757,638 - PP_0648 0.57 +0.9 757,638 - PP_0648 0.57 +1.3 757,639 + PP_0648 0.57 +1.5 757,639 + PP_0648 0.57 +0.8 757,640 - PP_0648 0.57 -0.3 757,640 - PP_0648 0.57 -0.3 757,641 - PP_0648 0.57 -3.4 757,673 + PP_0648 0.64 +1.4 757,674 - PP_0648 0.65 +1.3 757,674 - PP_0648 0.65 -0.2 757,693 + PP_0648 0.69 +1.6 757,756 + PP_0648 0.83 +2.4 757,756 + PP_0648 0.83 -1.8 757,757 - PP_0648 0.83 +1.5 757,757 - PP_0648 0.83 -4.6 757,767 + PP_0648 0.85 -3.3 757,767 + PP_0648 0.85 +1.0 757,803 - -0.1 757,838 + +1.1 757,880 + +1.5 757,880 + +0.8 757,880 + +3.0 757,900 + -0.2 757,900 + +0.6 757,900 + -0.0 757,947 + +1.0 757,947 + +1.0 757,948 - -0.9 757,948 - +1.4 757,948 - +2.0 757,948 - -1.6 757,948 - +0.2 757,980 + PP_0649 0.14 +0.5 757,998 + PP_0649 0.18 +2.1 757,998 + PP_0649 0.18 +1.1 757,999 - PP_0649 0.19 +1.1 758,087 - PP_0649 0.40 +0.1 758,087 - PP_0649 0.40 +1.2 758,189 - PP_0649 0.65 +0.7 758,189 - PP_0649 0.65 +0.3 758,306 - -1.9 758,330 + +1.1 758,331 - +0.3 758,331 - +2.1 758,331 - +0.8 758,331 - +1.8 758,340 + -3.3 758,340 + -4.3 758,341 - -3.6 758,341 - -0.4 758,358 - -1.6 758,384 + PP_0650 0.22 -3.8 758,384 + PP_0650 0.22 +0.9 758,385 - PP_0650 0.23 +0.4 758,385 - PP_0650 0.23 +0.9 758,387 + PP_0650 0.24 +1.2 758,387 + PP_0650 0.24 +0.5 758,388 - PP_0650 0.25 +0.9 758,388 - PP_0650 0.25 +1.6 758,388 - PP_0650 0.25 +0.7 758,388 - PP_0650 0.25 +0.3 758,446 - PP_0650 0.77 +1.2 758,446 - PP_0650 0.77 -0.6 758,451 + PP_0650 0.82 -0.6 758,451 + PP_0650 0.82 +1.3 758,451 + PP_0650 0.82 +0.9 758,452 - PP_0650 0.83 -0.2 758,452 - PP_0650 0.83 +1.8 758,486 + +0.4 758,486 + -3.9 758,486 + -1.5 758,514 + +1.7 758,514 + +1.4 758,515 - +1.0 758,538 + +0.5 758,539 - +0.5 758,539 - +0.7 758,539 - -4.1 758,560 - +1.8 758,562 - +1.2 758,626 - PP_0651 0.10 +0.3 758,626 - PP_0651 0.10 +2.4 758,638 + PP_0651 0.13 -4.5 758,638 + PP_0651 0.13 -2.2 758,639 - PP_0651 0.13 -3.8 758,640 + PP_0651 0.14 -0.8 758,640 + PP_0651 0.14 +0.3 758,737 + PP_0651 0.35 +1.0 758,738 - +1.4 758,757 + PP_0651 0.40 +1.1 758,757 + PP_0651 0.40 +2.0 758,758 - PP_0651 0.40 +2.0 758,758 - PP_0651 0.40 +1.1 758,763 + PP_0651 0.41 +1.8 758,764 - PP_0651 0.41 +0.4
Or see this region's nucleotide sequence