Experiment: P. putida Sci small MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0335 and PP_0336 are separated by 3 nucleotides PP_0336 and PP_0337 are separated by 168 nucleotides PP_0337 and PP_0338 are separated by 307 nucleotides
PP_0335: PP_0335 - Glutathione S-transferase family protein, at 401,902 to 402,525
_0335
PP_0336: PP_0336 - Peptide methionine sulfoxide reductase MsrA, at 402,529 to 403,197
_0336
PP_0337: PP_0337 - Sensory box protein, at 403,366 to 406,056
_0337
PP_0338: PP_0338 - AceF-S-acetyldihydrolipoate, at 406,364 to 408,004
_0338
Position (kb) 403
404
405
406
407 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 402.412 kb on + strand, within PP_0335 at 402.412 kb on + strand, within PP_0335 at 402.412 kb on + strand, within PP_0335 at 402.412 kb on + strand, within PP_0335 at 402.412 kb on + strand, within PP_0335 at 402.413 kb on - strand, within PP_0335 at 402.413 kb on - strand, within PP_0335 at 402.413 kb on - strand, within PP_0335 at 402.413 kb on - strand, within PP_0335 at 402.534 kb on + strand at 402.608 kb on + strand, within PP_0336 at 402.608 kb on + strand, within PP_0336 at 402.621 kb on - strand, within PP_0336 at 402.635 kb on + strand, within PP_0336 at 402.755 kb on + strand, within PP_0336 at 402.755 kb on + strand, within PP_0336 at 402.756 kb on - strand, within PP_0336 at 402.756 kb on - strand, within PP_0336 at 402.756 kb on - strand, within PP_0336 at 402.852 kb on - strand, within PP_0336 at 402.926 kb on + strand, within PP_0336 at 402.926 kb on + strand, within PP_0336 at 402.926 kb on + strand, within PP_0336 at 402.926 kb on + strand, within PP_0336 at 402.926 kb on + strand, within PP_0336 at 402.927 kb on - strand, within PP_0336 at 402.927 kb on - strand, within PP_0336 at 402.930 kb on + strand, within PP_0336 at 402.930 kb on + strand, within PP_0336 at 402.930 kb on + strand, within PP_0336 at 402.930 kb on + strand, within PP_0336 at 402.930 kb on + strand, within PP_0336 at 403.094 kb on + strand, within PP_0336 at 403.094 kb on + strand, within PP_0336 at 403.095 kb on - strand, within PP_0336 at 403.095 kb on - strand, within PP_0336 at 403.095 kb on - strand, within PP_0336 at 403.216 kb on - strand at 403.267 kb on + strand at 403.268 kb on - strand at 403.268 kb on - strand at 403.268 kb on - strand at 403.340 kb on + strand at 403.340 kb on + strand at 403.340 kb on + strand at 403.340 kb on + strand at 403.340 kb on + strand at 403.341 kb on - strand at 403.341 kb on - strand at 403.343 kb on - strand at 403.343 kb on - strand at 403.356 kb on + strand at 403.356 kb on + strand at 403.389 kb on - strand at 403.442 kb on + strand at 403.641 kb on + strand, within PP_0337 at 403.874 kb on - strand, within PP_0337 at 403.874 kb on - strand, within PP_0337 at 403.874 kb on - strand, within PP_0337 at 403.874 kb on - strand, within PP_0337 at 404.087 kb on + strand, within PP_0337 at 404.087 kb on + strand, within PP_0337 at 404.088 kb on - strand, within PP_0337 at 404.088 kb on - strand, within PP_0337 at 404.088 kb on - strand, within PP_0337 at 404.150 kb on + strand, within PP_0337 at 404.150 kb on + strand, within PP_0337 at 404.151 kb on - strand, within PP_0337 at 404.189 kb on - strand, within PP_0337 at 404.199 kb on + strand, within PP_0337 at 404.651 kb on + strand, within PP_0337 at 404.651 kb on + strand, within PP_0337 at 404.652 kb on - strand, within PP_0337 at 404.705 kb on + strand, within PP_0337 at 404.717 kb on + strand, within PP_0337 at 404.717 kb on + strand, within PP_0337 at 404.717 kb on + strand, within PP_0337 at 404.717 kb on + strand, within PP_0337 at 404.718 kb on - strand, within PP_0337 at 404.718 kb on - strand, within PP_0337 at 404.718 kb on - strand, within PP_0337 at 404.745 kb on - strand, within PP_0337 at 404.775 kb on - strand, within PP_0337 at 404.924 kb on + strand, within PP_0337 at 404.924 kb on + strand, within PP_0337 at 404.925 kb on - strand, within PP_0337 at 404.925 kb on - strand, within PP_0337 at 404.957 kb on + strand, within PP_0337 at 404.958 kb on - strand, within PP_0337 at 404.958 kb on - strand, within PP_0337 at 404.958 kb on - strand, within PP_0337 at 405.434 kb on + strand, within PP_0337 at 405.497 kb on + strand, within PP_0337 at 405.522 kb on + strand, within PP_0337 at 405.523 kb on - strand, within PP_0337 at 405.716 kb on + strand, within PP_0337 at 405.716 kb on + strand, within PP_0337 at 405.717 kb on - strand, within PP_0337 at 405.770 kb on + strand, within PP_0337 at 405.771 kb on - strand, within PP_0337 at 405.783 kb on - strand, within PP_0337 at 405.783 kb on - strand, within PP_0337 at 405.831 kb on - strand at 405.932 kb on + strand at 405.933 kb on - strand at 406.001 kb on - strand at 406.001 kb on - strand at 406.305 kb on + strand at 406.305 kb on + strand at 406.365 kb on + strand at 406.779 kb on + strand, within PP_0338 at 406.779 kb on + strand, within PP_0338
Per-strain Table
Position Strand Gene LocusTag Fraction P. putida Sci small MOI 0.1 remove 402,412 + PP_0335 0.82 -0.6 402,412 + PP_0335 0.82 +0.5 402,412 + PP_0335 0.82 -3.8 402,412 + PP_0335 0.82 -0.7 402,412 + PP_0335 0.82 +1.2 402,413 - PP_0335 0.82 +2.8 402,413 - PP_0335 0.82 +2.4 402,413 - PP_0335 0.82 -3.0 402,413 - PP_0335 0.82 -3.0 402,534 + +1.1 402,608 + PP_0336 0.12 -0.8 402,608 + PP_0336 0.12 +0.3 402,621 - PP_0336 0.14 +0.8 402,635 + PP_0336 0.16 +2.3 402,755 + PP_0336 0.34 -0.2 402,755 + PP_0336 0.34 +1.8 402,756 - PP_0336 0.34 -4.6 402,756 - PP_0336 0.34 +0.9 402,756 - PP_0336 0.34 -4.4 402,852 - PP_0336 0.48 -4.4 402,926 + PP_0336 0.59 +1.2 402,926 + PP_0336 0.59 -2.5 402,926 + PP_0336 0.59 -4.0 402,926 + PP_0336 0.59 -3.2 402,926 + PP_0336 0.59 -4.3 402,927 - PP_0336 0.59 +0.8 402,927 - PP_0336 0.59 -0.1 402,930 + PP_0336 0.60 +1.3 402,930 + PP_0336 0.60 -0.2 402,930 + PP_0336 0.60 +0.2 402,930 + PP_0336 0.60 -1.5 402,930 + PP_0336 0.60 +1.2 403,094 + PP_0336 0.84 +1.9 403,094 + PP_0336 0.84 +0.5 403,095 - PP_0336 0.85 +0.6 403,095 - PP_0336 0.85 -4.2 403,095 - PP_0336 0.85 +0.7 403,216 - +0.6 403,267 + -4.0 403,268 - +1.9 403,268 - -1.7 403,268 - +1.5 403,340 + -0.5 403,340 + +1.0 403,340 + -0.2 403,340 + -0.3 403,340 + +0.7 403,341 - -2.9 403,341 - -4.0 403,343 - +1.6 403,343 - -0.5 403,356 + +1.9 403,356 + +1.1 403,389 - -1.0 403,442 + -1.5 403,641 + PP_0337 0.10 -4.1 403,874 - PP_0337 0.19 -2.8 403,874 - PP_0337 0.19 -0.5 403,874 - PP_0337 0.19 -4.4 403,874 - PP_0337 0.19 -3.4 404,087 + PP_0337 0.27 +0.6 404,087 + PP_0337 0.27 +0.8 404,088 - PP_0337 0.27 +1.1 404,088 - PP_0337 0.27 +1.8 404,088 - PP_0337 0.27 +1.3 404,150 + PP_0337 0.29 -2.7 404,150 + PP_0337 0.29 +1.0 404,151 - PP_0337 0.29 +1.6 404,189 - PP_0337 0.31 -0.6 404,199 + PP_0337 0.31 +1.4 404,651 + PP_0337 0.48 +0.9 404,651 + PP_0337 0.48 -0.5 404,652 - PP_0337 0.48 -4.6 404,705 + PP_0337 0.50 -1.1 404,717 + PP_0337 0.50 +0.3 404,717 + PP_0337 0.50 +0.4 404,717 + PP_0337 0.50 -3.2 404,717 + PP_0337 0.50 -1.5 404,718 - PP_0337 0.50 -4.3 404,718 - PP_0337 0.50 +0.7 404,718 - PP_0337 0.50 +0.2 404,745 - PP_0337 0.51 -4.6 404,775 - PP_0337 0.52 +0.7 404,924 + PP_0337 0.58 +1.8 404,924 + PP_0337 0.58 -3.7 404,925 - PP_0337 0.58 -3.9 404,925 - PP_0337 0.58 -0.1 404,957 + PP_0337 0.59 +0.1 404,958 - PP_0337 0.59 +0.4 404,958 - PP_0337 0.59 +0.9 404,958 - PP_0337 0.59 +0.6 405,434 + PP_0337 0.77 +1.9 405,497 + PP_0337 0.79 +0.5 405,522 + PP_0337 0.80 +1.5 405,523 - PP_0337 0.80 -4.0 405,716 + PP_0337 0.87 -0.2 405,716 + PP_0337 0.87 +1.4 405,717 - PP_0337 0.87 +1.2 405,770 + PP_0337 0.89 +0.9 405,771 - PP_0337 0.89 -1.2 405,783 - PP_0337 0.90 -0.1 405,783 - PP_0337 0.90 +1.2 405,831 - +0.1 405,932 + +0.9 405,933 - +0.3 406,001 - -3.7 406,001 - -1.4 406,305 + -3.3 406,305 + +2.0 406,365 + +0.5 406,779 + PP_0338 0.25 +0.5 406,779 + PP_0338 0.25 +0.5
Or see this region's nucleotide sequence