Strain Fitness in Escherichia coli BW25113 around b4513

Experiment: LB + carbenicillin Time4hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntkdpA and kdpF overlap by 1 nucleotideskdpF and ybfA are separated by 312 nucleotidesybfA and rhsC are separated by 242 nucleotides b0698: kdpA - potassium-transporting ATPase subunit A (NCBI), at 726,282 to 727,955 kdpA b4513: kdpF - potassium ion accessory transporter subunit (NCBI), at 727,955 to 728,044 kdpF b0699: ybfA - hypothetical protein (NCBI), at 728,357 to 728,563 ybfA b0700: rhsC - rhsC element core protein RshC (NCBI), at 728,806 to 732,999 rhsC Position (kb) 727 728 729Strain fitness (log2 ratio) -1 0 1at 726.981 kb on + strand, within kdpAat 727.062 kb on - strand, within kdpAat 727.075 kb on - strand, within kdpAat 727.075 kb on - strand, within kdpAat 727.107 kb on - strand, within kdpAat 727.325 kb on - strand, within kdpAat 727.338 kb on + strand, within kdpAat 727.338 kb on + strand, within kdpAat 727.435 kb on - strand, within kdpAat 727.464 kb on + strand, within kdpAat 727.600 kb on + strand, within kdpAat 727.600 kb on + strand, within kdpAat 727.640 kb on - strand, within kdpAat 727.687 kb on - strand, within kdpAat 727.692 kb on - strand, within kdpAat 727.696 kb on + strand, within kdpAat 727.696 kb on + strand, within kdpAat 727.696 kb on + strand, within kdpAat 727.767 kb on + strand, within kdpAat 727.792 kb on + strandat 727.871 kb on + strandat 727.871 kb on + strandat 727.884 kb on + strandat 727.904 kb on - strandat 727.904 kb on - strandat 727.963 kb on + strandat 727.966 kb on - strandat 727.988 kb on - strand, within kdpFat 728.022 kb on - strand, within kdpFat 728.054 kb on - strandat 728.054 kb on - strandat 728.059 kb on - strandat 728.068 kb on - strandat 728.076 kb on - strandat 728.580 kb on + strandat 728.597 kb on + strandat 728.641 kb on + strandat 728.641 kb on + strandat 728.758 kb on - strandat 728.810 kb on - strandat 728.840 kb on - strandat 728.863 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time4hr
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726,981 + kdpA b0698 0.42 -0.0
727,062 - kdpA b0698 0.47 -0.2
727,075 - kdpA b0698 0.47 +0.0
727,075 - kdpA b0698 0.47 -0.4
727,107 - kdpA b0698 0.49 -0.3
727,325 - kdpA b0698 0.62 +0.2
727,338 + kdpA b0698 0.63 +0.1
727,338 + kdpA b0698 0.63 -0.3
727,435 - kdpA b0698 0.69 -0.2
727,464 + kdpA b0698 0.71 -0.0
727,600 + kdpA b0698 0.79 +0.0
727,600 + kdpA b0698 0.79 +0.2
727,640 - kdpA b0698 0.81 -0.1
727,687 - kdpA b0698 0.84 -0.4
727,692 - kdpA b0698 0.84 +0.4
727,696 + kdpA b0698 0.84 -0.6
727,696 + kdpA b0698 0.84 +0.7
727,696 + kdpA b0698 0.84 -0.4
727,767 + kdpA b0698 0.89 -0.0
727,792 + -0.1
727,871 + -0.6
727,871 + -1.2
727,884 + +0.2
727,904 - +0.2
727,904 - +0.0
727,963 + +0.0
727,966 - -1.6
727,988 - kdpF b4513 0.37 +0.7
728,022 - kdpF b4513 0.74 -0.2
728,054 - +0.4
728,054 - +0.6
728,059 - +0.1
728,068 - +0.6
728,076 - +0.3
728,580 + +0.3
728,597 + -0.3
728,641 + -0.2
728,641 + +0.6
728,758 - -0.1
728,810 - +0.6
728,840 - +0.1
728,863 - +0.2

Or see this region's nucleotide sequence