Strain Fitness in Escherichia coli BW25113 around b3017

Experiment: LB + carbenicillin Time4hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygiQ and sufI are separated by 110 nucleotidessufI and plsC are separated by 74 nucleotides b4469: ygiQ - hypothetical protein (NCBI), at 3,156,949 to 3,159,168 ygiQ b3017: sufI - repressor protein for FtsI (NCBI), at 3,159,279 to 3,160,691 sufI b3018: plsC - 1-acyl-sn-glycerol-3-phosphate acyltransferase (NCBI), at 3,160,766 to 3,161,503 plsC Position (kb) 3159 3160 3161Strain fitness (log2 ratio) -1 0 1at 3158.313 kb on + strand, within ygiQat 3158.313 kb on + strand, within ygiQat 3158.313 kb on + strand, within ygiQat 3158.336 kb on + strand, within ygiQat 3158.336 kb on + strand, within ygiQat 3158.344 kb on - strand, within ygiQat 3158.395 kb on + strand, within ygiQat 3158.395 kb on + strand, within ygiQat 3158.395 kb on + strand, within ygiQat 3158.448 kb on + strand, within ygiQat 3158.448 kb on + strand, within ygiQat 3158.472 kb on - strand, within ygiQat 3158.509 kb on - strand, within ygiQat 3158.511 kb on - strand, within ygiQat 3158.511 kb on - strand, within ygiQat 3158.568 kb on - strand, within ygiQat 3158.614 kb on + strand, within ygiQat 3158.634 kb on + strand, within ygiQat 3158.650 kb on - strand, within ygiQat 3158.650 kb on - strand, within ygiQat 3158.658 kb on + strand, within ygiQat 3158.658 kb on + strand, within ygiQat 3158.680 kb on - strand, within ygiQat 3158.735 kb on + strand, within ygiQat 3158.740 kb on + strand, within ygiQat 3158.757 kb on - strand, within ygiQat 3158.757 kb on - strand, within ygiQat 3158.783 kb on + strand, within ygiQat 3159.010 kb on - strandat 3159.057 kb on - strandat 3159.057 kb on - strandat 3159.062 kb on - strandat 3159.062 kb on - strandat 3159.107 kb on + strandat 3159.107 kb on + strandat 3159.143 kb on + strandat 3159.250 kb on - strandat 3159.250 kb on - strandat 3159.277 kb on - strandat 3159.302 kb on - strandat 3159.302 kb on - strandat 3159.308 kb on - strandat 3159.314 kb on + strandat 3159.314 kb on + strandat 3159.378 kb on - strandat 3159.388 kb on + strandat 3159.422 kb on - strand, within sufIat 3159.500 kb on - strand, within sufIat 3159.500 kb on - strand, within sufIat 3159.537 kb on + strand, within sufIat 3159.540 kb on - strand, within sufIat 3159.540 kb on - strand, within sufIat 3159.550 kb on - strand, within sufIat 3159.574 kb on - strand, within sufIat 3159.583 kb on + strand, within sufIat 3159.591 kb on - strand, within sufIat 3159.620 kb on + strand, within sufIat 3159.620 kb on + strand, within sufIat 3159.624 kb on + strand, within sufIat 3159.737 kb on + strand, within sufIat 3159.768 kb on - strand, within sufIat 3159.769 kb on - strand, within sufIat 3159.769 kb on - strand, within sufIat 3159.785 kb on + strand, within sufIat 3159.785 kb on + strand, within sufIat 3159.818 kb on + strand, within sufIat 3159.818 kb on + strand, within sufIat 3159.837 kb on - strand, within sufIat 3159.959 kb on + strand, within sufIat 3159.970 kb on - strand, within sufIat 3160.019 kb on - strand, within sufIat 3160.028 kb on - strand, within sufIat 3160.236 kb on + strand, within sufIat 3160.266 kb on - strand, within sufIat 3160.266 kb on - strand, within sufIat 3160.269 kb on + strand, within sufIat 3160.269 kb on + strand, within sufIat 3160.270 kb on + strand, within sufIat 3160.272 kb on + strand, within sufIat 3160.287 kb on + strand, within sufIat 3160.308 kb on - strand, within sufIat 3160.335 kb on + strand, within sufIat 3160.370 kb on + strand, within sufIat 3160.371 kb on + strand, within sufIat 3160.494 kb on - strand, within sufIat 3160.494 kb on - strand, within sufIat 3160.518 kb on - strand, within sufIat 3160.524 kb on + strand, within sufIat 3160.538 kb on - strand, within sufIat 3160.540 kb on + strand, within sufIat 3160.607 kb on - strandat 3160.620 kb on - strandat 3160.634 kb on + strandat 3160.634 kb on + strandat 3160.634 kb on + strandat 3160.637 kb on - strandat 3160.685 kb on + strandat 3160.685 kb on + strandat 3160.687 kb on + strandat 3160.691 kb on + strandat 3160.701 kb on + strandat 3160.716 kb on + strandat 3160.724 kb on - strandat 3160.726 kb on - strandat 3161.508 kb on - strandat 3161.532 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time4hr
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3,158,313 + ygiQ b4469 0.61 +0.2
3,158,313 + ygiQ b4469 0.61 -0.2
3,158,313 + ygiQ b4469 0.61 -0.2
3,158,336 + ygiQ b4469 0.62 +0.6
3,158,336 + ygiQ b4469 0.62 -0.5
3,158,344 - ygiQ b4469 0.63 -0.1
3,158,395 + ygiQ b4469 0.65 -0.8
3,158,395 + ygiQ b4469 0.65 -0.0
3,158,395 + ygiQ b4469 0.65 -0.1
3,158,448 + ygiQ b4469 0.68 +0.1
3,158,448 + ygiQ b4469 0.68 +0.2
3,158,472 - ygiQ b4469 0.69 -0.8
3,158,509 - ygiQ b4469 0.70 -0.4
3,158,511 - ygiQ b4469 0.70 -0.5
3,158,511 - ygiQ b4469 0.70 -0.3
3,158,568 - ygiQ b4469 0.73 +0.3
3,158,614 + ygiQ b4469 0.75 -0.3
3,158,634 + ygiQ b4469 0.76 +0.0
3,158,650 - ygiQ b4469 0.77 -0.1
3,158,650 - ygiQ b4469 0.77 +0.0
3,158,658 + ygiQ b4469 0.77 +0.6
3,158,658 + ygiQ b4469 0.77 -0.3
3,158,680 - ygiQ b4469 0.78 +0.3
3,158,735 + ygiQ b4469 0.80 -0.1
3,158,740 + ygiQ b4469 0.81 +0.6
3,158,757 - ygiQ b4469 0.81 +0.0
3,158,757 - ygiQ b4469 0.81 -0.2
3,158,783 + ygiQ b4469 0.83 +0.1
3,159,010 - +0.2
3,159,057 - -0.1
3,159,057 - +0.3
3,159,062 - -0.6
3,159,062 - +0.6
3,159,107 + +0.7
3,159,107 + -0.3
3,159,143 + -0.5
3,159,250 - -0.3
3,159,250 - -0.2
3,159,277 - +0.3
3,159,302 - +0.5
3,159,302 - -0.0
3,159,308 - +0.4
3,159,314 + +0.5
3,159,314 + +0.6
3,159,378 - -0.1
3,159,388 + -0.3
3,159,422 - sufI b3017 0.10 +0.2
3,159,500 - sufI b3017 0.16 +0.7
3,159,500 - sufI b3017 0.16 +0.4
3,159,537 + sufI b3017 0.18 +0.2
3,159,540 - sufI b3017 0.18 +1.0
3,159,540 - sufI b3017 0.18 -0.3
3,159,550 - sufI b3017 0.19 +0.7
3,159,574 - sufI b3017 0.21 +0.1
3,159,583 + sufI b3017 0.22 +0.2
3,159,591 - sufI b3017 0.22 +0.4
3,159,620 + sufI b3017 0.24 +0.1
3,159,620 + sufI b3017 0.24 +0.8
3,159,624 + sufI b3017 0.24 +0.5
3,159,737 + sufI b3017 0.32 -0.6
3,159,768 - sufI b3017 0.35 -0.1
3,159,769 - sufI b3017 0.35 +0.1
3,159,769 - sufI b3017 0.35 -0.1
3,159,785 + sufI b3017 0.36 -0.2
3,159,785 + sufI b3017 0.36 +0.5
3,159,818 + sufI b3017 0.38 +0.8
3,159,818 + sufI b3017 0.38 +0.1
3,159,837 - sufI b3017 0.39 +0.4
3,159,959 + sufI b3017 0.48 +0.1
3,159,970 - sufI b3017 0.49 -0.3
3,160,019 - sufI b3017 0.52 +0.1
3,160,028 - sufI b3017 0.53 +0.4
3,160,236 + sufI b3017 0.68 +0.2
3,160,266 - sufI b3017 0.70 +0.2
3,160,266 - sufI b3017 0.70 +0.9
3,160,269 + sufI b3017 0.70 -0.0
3,160,269 + sufI b3017 0.70 -0.0
3,160,270 + sufI b3017 0.70 +0.2
3,160,272 + sufI b3017 0.70 -0.2
3,160,287 + sufI b3017 0.71 +0.2
3,160,308 - sufI b3017 0.73 +0.3
3,160,335 + sufI b3017 0.75 +0.1
3,160,370 + sufI b3017 0.77 +0.2
3,160,371 + sufI b3017 0.77 +0.1
3,160,494 - sufI b3017 0.86 +0.5
3,160,494 - sufI b3017 0.86 +0.3
3,160,518 - sufI b3017 0.88 -0.4
3,160,524 + sufI b3017 0.88 +0.4
3,160,538 - sufI b3017 0.89 +0.1
3,160,540 + sufI b3017 0.89 +0.3
3,160,607 - +0.3
3,160,620 - +0.1
3,160,634 + -0.2
3,160,634 + +0.2
3,160,634 + -0.3
3,160,637 - +0.4
3,160,685 + +0.3
3,160,685 + +0.5
3,160,687 + -0.1
3,160,691 + +0.1
3,160,701 + +0.0
3,160,716 + +0.3
3,160,724 - +0.2
3,160,726 - -0.3
3,161,508 - -0.1
3,161,532 + -0.2

Or see this region's nucleotide sequence