Strain Fitness in Escherichia coli BW25113 around b2736

Experiment: LB + carbenicillin Time4hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygbI and ygbJ are separated by 195 nucleotidesygbJ and ygbK overlap by 4 nucleotides b2735: ygbI - predicted DNA-binding transcriptional regulator (RefSeq), at 2,858,489 to 2,859,256 ygbI b2736: ygbJ - predicted dehydrogenase, with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,859,452 to 2,860,360 ygbJ b2737: ygbK - hypothetical protein (NCBI), at 2,860,357 to 2,861,523 ygbK Position (kb) 2859 2860 2861Strain fitness (log2 ratio) -1 0 1 2at 2858.457 kb on + strandat 2858.457 kb on - strandat 2858.466 kb on - strandat 2858.471 kb on - strandat 2858.471 kb on - strandat 2858.473 kb on - strandat 2858.476 kb on - strandat 2858.514 kb on + strandat 2858.518 kb on + strandat 2858.568 kb on + strand, within ygbIat 2858.568 kb on + strand, within ygbIat 2858.618 kb on - strand, within ygbIat 2858.719 kb on + strand, within ygbIat 2858.822 kb on - strand, within ygbIat 2858.827 kb on - strand, within ygbIat 2858.827 kb on - strand, within ygbIat 2858.912 kb on - strand, within ygbIat 2859.114 kb on - strand, within ygbIat 2859.160 kb on - strand, within ygbIat 2859.528 kb on - strandat 2859.528 kb on - strandat 2859.539 kb on - strandat 2859.664 kb on + strand, within ygbJat 2859.852 kb on - strand, within ygbJat 2859.962 kb on - strand, within ygbJat 2859.965 kb on + strand, within ygbJat 2860.243 kb on + strand, within ygbJat 2860.243 kb on + strand, within ygbJat 2860.296 kb on + strandat 2860.464 kb on - strandat 2860.540 kb on - strand, within ygbKat 2860.598 kb on - strand, within ygbKat 2860.598 kb on - strand, within ygbKat 2860.657 kb on + strand, within ygbKat 2860.721 kb on + strand, within ygbKat 2860.847 kb on - strand, within ygbKat 2860.851 kb on + strand, within ygbKat 2860.895 kb on - strand, within ygbKat 2860.990 kb on + strand, within ygbKat 2860.990 kb on + strand, within ygbKat 2860.997 kb on + strand, within ygbKat 2860.998 kb on - strand, within ygbKat 2861.029 kb on - strand, within ygbKat 2861.044 kb on + strand, within ygbKat 2861.044 kb on + strand, within ygbKat 2861.080 kb on + strand, within ygbKat 2861.111 kb on + strand, within ygbKat 2861.166 kb on - strand, within ygbKat 2861.195 kb on + strand, within ygbKat 2861.301 kb on + strand, within ygbKat 2861.301 kb on + strand, within ygbK

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time4hr
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2,858,457 + +1.1
2,858,457 - -0.3
2,858,466 - -0.3
2,858,471 - -0.0
2,858,471 - +0.1
2,858,473 - +0.2
2,858,476 - -0.3
2,858,514 + -1.5
2,858,518 + +1.8
2,858,568 + ygbI b2735 0.10 -0.1
2,858,568 + ygbI b2735 0.10 -0.1
2,858,618 - ygbI b2735 0.17 -0.3
2,858,719 + ygbI b2735 0.30 +0.1
2,858,822 - ygbI b2735 0.43 -0.1
2,858,827 - ygbI b2735 0.44 -1.4
2,858,827 - ygbI b2735 0.44 +0.3
2,858,912 - ygbI b2735 0.55 -0.4
2,859,114 - ygbI b2735 0.81 -0.2
2,859,160 - ygbI b2735 0.87 -0.2
2,859,528 - -0.1
2,859,528 - +0.1
2,859,539 - -0.2
2,859,664 + ygbJ b2736 0.23 +0.3
2,859,852 - ygbJ b2736 0.44 -0.1
2,859,962 - ygbJ b2736 0.56 +0.2
2,859,965 + ygbJ b2736 0.56 -0.2
2,860,243 + ygbJ b2736 0.87 +0.4
2,860,243 + ygbJ b2736 0.87 +1.0
2,860,296 + +0.0
2,860,464 - +0.1
2,860,540 - ygbK b2737 0.16 -0.5
2,860,598 - ygbK b2737 0.21 -0.0
2,860,598 - ygbK b2737 0.21 +0.1
2,860,657 + ygbK b2737 0.26 -0.0
2,860,721 + ygbK b2737 0.31 +0.4
2,860,847 - ygbK b2737 0.42 -0.6
2,860,851 + ygbK b2737 0.42 -0.1
2,860,895 - ygbK b2737 0.46 -0.4
2,860,990 + ygbK b2737 0.54 +0.6
2,860,990 + ygbK b2737 0.54 +0.3
2,860,997 + ygbK b2737 0.55 +0.2
2,860,998 - ygbK b2737 0.55 +0.1
2,861,029 - ygbK b2737 0.58 +0.0
2,861,044 + ygbK b2737 0.59 +0.3
2,861,044 + ygbK b2737 0.59 +0.2
2,861,080 + ygbK b2737 0.62 +0.1
2,861,111 + ygbK b2737 0.65 -0.0
2,861,166 - ygbK b2737 0.69 -0.2
2,861,195 + ygbK b2737 0.72 +0.1
2,861,301 + ygbK b2737 0.81 -0.1
2,861,301 + ygbK b2737 0.81 +0.2

Or see this region's nucleotide sequence