Strain Fitness in Escherichia coli BW25113 around b1958

Experiment: LB + carbenicillin Time4hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb1956 and yodC are separated by 170 nucleotidesyodC and yedI are separated by 78 nucleotidesyedI and yedA are separated by 172 nucleotides b1956: b1956 - orf, hypothetical protein (VIMSS), at 2,024,347 to 2,026,041 b1956 b1957: yodC - hypothetical protein (NCBI), at 2,026,212 to 2,026,394 yodC b1958: yedI - conserved inner membrane protein (NCBI), at 2,026,473 to 2,027,390 yedI b1959: yedA - predicted inner membrane protein (NCBI), at 2,027,563 to 2,028,483 yedA Position (kb) 2026 2027 2028Strain fitness (log2 ratio) -1 0 1at 2025.574 kb on + strand, within b1956at 2025.591 kb on + strand, within b1956at 2025.627 kb on - strand, within b1956at 2025.848 kb on + strand, within b1956at 2025.848 kb on + strand, within b1956at 2025.893 kb on + strandat 2026.076 kb on + strandat 2026.320 kb on - strand, within yodCat 2026.382 kb on + strandat 2026.439 kb on - strandat 2026.470 kb on + strandat 2026.610 kb on - strand, within yedIat 2026.657 kb on - strand, within yedIat 2026.834 kb on + strand, within yedIat 2026.859 kb on - strand, within yedIat 2026.880 kb on + strand, within yedIat 2026.906 kb on - strand, within yedIat 2027.009 kb on - strand, within yedIat 2027.080 kb on - strand, within yedIat 2027.285 kb on - strand, within yedIat 2027.321 kb on - strandat 2027.339 kb on - strandat 2027.339 kb on - strandat 2027.416 kb on - strandat 2027.416 kb on - strandat 2027.424 kb on - strandat 2027.450 kb on - strandat 2027.459 kb on - strandat 2027.608 kb on + strandat 2027.686 kb on + strand, within yedAat 2027.723 kb on + strand, within yedAat 2028.038 kb on + strand, within yedAat 2028.081 kb on - strand, within yedAat 2028.322 kb on + strand, within yedA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time4hr
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2,025,574 + b1956 b1956 0.72 -0.0
2,025,591 + b1956 b1956 0.73 +0.1
2,025,627 - b1956 b1956 0.76 +0.1
2,025,848 + b1956 b1956 0.89 +0.1
2,025,848 + b1956 b1956 0.89 -0.1
2,025,893 + -0.3
2,026,076 + -0.9
2,026,320 - yodC b1957 0.59 -0.2
2,026,382 + +0.3
2,026,439 - +0.5
2,026,470 + -0.4
2,026,610 - yedI b1958 0.15 +0.5
2,026,657 - yedI b1958 0.20 -0.0
2,026,834 + yedI b1958 0.39 +1.1
2,026,859 - yedI b1958 0.42 +0.3
2,026,880 + yedI b1958 0.44 +0.1
2,026,906 - yedI b1958 0.47 +0.7
2,027,009 - yedI b1958 0.58 -0.1
2,027,080 - yedI b1958 0.66 +0.5
2,027,285 - yedI b1958 0.88 -0.1
2,027,321 - -0.2
2,027,339 - -0.1
2,027,339 - -0.4
2,027,416 - +0.7
2,027,416 - +0.4
2,027,424 - +0.4
2,027,450 - -0.1
2,027,459 - +0.1
2,027,608 + -0.1
2,027,686 + yedA b1959 0.13 +0.0
2,027,723 + yedA b1959 0.17 +0.1
2,028,038 + yedA b1959 0.52 +0.4
2,028,081 - yedA b1959 0.56 +0.1
2,028,322 + yedA b1959 0.82 +0.2

Or see this region's nucleotide sequence