Strain Fitness in Escherichia coli BW25113 around b1172

Experiment: LB + carbenicillin Time4hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycgH and b1171 are separated by 56 nucleotidesb1171 and ymgG are separated by 9 nucleotidesymgG and ymgI are separated by 1 nucleotidesymgI and ymgJ are separated by 100 nucleotidesymgJ and ycgI are separated by 114 nucleotides b4491: ycgH - no description, at 1,218,824 to 1,221,471 ycgH b1171: b1171 - orf, hypothetical protein (VIMSS), at 1,221,528 to 1,221,857 b1171 b1172: ymgG - conserved protein, UPF0757 family (RefSeq), at 1,221,867 to 1,222,211 ymgG b4593: ymgI - hypothetical protein (RefSeq), at 1,222,213 to 1,222,386 ymgI b4594: ymgJ - hypothetical protein (RefSeq), at 1,222,487 to 1,222,672 ymgJ b4521: ycgI - hypothetical protein (NCBI), at 1,222,787 to 1,223,130 ycgI Position (kb) 1221 1222 1223Strain fitness (log2 ratio) -2 -1 0 1 2at 1220.907 kb on + strand, within ycgHat 1220.907 kb on + strand, within ycgHat 1220.922 kb on - strand, within ycgHat 1220.932 kb on - strand, within ycgHat 1220.932 kb on - strand, within ycgHat 1221.029 kb on - strand, within ycgHat 1221.103 kb on - strand, within ycgHat 1221.128 kb on + strand, within ycgHat 1221.137 kb on + strand, within ycgHat 1221.137 kb on + strand, within ycgHat 1221.271 kb on + strandat 1221.306 kb on + strandat 1221.396 kb on + strandat 1221.396 kb on + strandat 1221.424 kb on - strandat 1221.429 kb on + strandat 1221.429 kb on + strandat 1221.440 kb on + strandat 1221.440 kb on + strandat 1221.517 kb on - strandat 1221.549 kb on - strandat 1221.551 kb on + strandat 1221.552 kb on + strandat 1221.553 kb on + strandat 1221.553 kb on - strandat 1221.635 kb on + strand, within b1171at 1221.815 kb on - strand, within b1171at 1221.815 kb on - strand, within b1171at 1221.892 kb on - strandat 1221.934 kb on - strand, within ymgGat 1221.940 kb on + strand, within ymgGat 1222.035 kb on + strand, within ymgGat 1222.052 kb on + strand, within ymgGat 1222.060 kb on - strand, within ymgGat 1222.060 kb on - strand, within ymgGat 1222.135 kb on - strand, within ymgGat 1222.137 kb on + strand, within ymgGat 1222.141 kb on + strand, within ymgGat 1222.152 kb on + strand, within ymgGat 1222.160 kb on - strand, within ymgGat 1222.160 kb on - strand, within ymgGat 1222.162 kb on + strand, within ymgGat 1222.162 kb on + strand, within ymgGat 1222.162 kb on + strand, within ymgGat 1222.162 kb on + strand, within ymgGat 1222.162 kb on + strand, within ymgGat 1222.167 kb on + strand, within ymgGat 1222.170 kb on - strand, within ymgGat 1222.170 kb on - strand, within ymgGat 1222.180 kb on - strandat 1222.219 kb on + strandat 1222.243 kb on - strand, within ymgIat 1222.283 kb on - strand, within ymgIat 1222.330 kb on - strand, within ymgIat 1222.383 kb on - strandat 1222.415 kb on + strandat 1222.423 kb on + strandat 1222.423 kb on + strandat 1222.436 kb on + strandat 1222.512 kb on + strand, within ymgJat 1222.568 kb on + strand, within ymgJat 1222.571 kb on - strand, within ymgJat 1222.571 kb on - strand, within ymgJat 1222.583 kb on - strand, within ymgJat 1222.630 kb on - strand, within ymgJat 1222.630 kb on - strand, within ymgJat 1222.659 kb on - strandat 1222.703 kb on - strandat 1222.703 kb on - strandat 1222.703 kb on - strandat 1222.722 kb on - strandat 1222.753 kb on + strandat 1222.760 kb on + strandat 1222.760 kb on + strandat 1222.763 kb on + strandat 1222.768 kb on - strandat 1222.783 kb on + strandat 1222.846 kb on + strand, within ycgIat 1222.857 kb on - strand, within ycgIat 1222.981 kb on + strand, within ycgIat 1223.000 kb on + strand, within ycgIat 1223.000 kb on + strand, within ycgI

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time4hr
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1,220,907 + ycgH b4491 0.79 +0.4
1,220,907 + ycgH b4491 0.79 -0.1
1,220,922 - ycgH b4491 0.79 +0.1
1,220,932 - ycgH b4491 0.80 -0.0
1,220,932 - ycgH b4491 0.80 -0.3
1,221,029 - ycgH b4491 0.83 +0.4
1,221,103 - ycgH b4491 0.86 +0.3
1,221,128 + ycgH b4491 0.87 +0.2
1,221,137 + ycgH b4491 0.87 +0.3
1,221,137 + ycgH b4491 0.87 -0.5
1,221,271 + +0.1
1,221,306 + -0.1
1,221,396 + -0.0
1,221,396 + -0.2
1,221,424 - +1.0
1,221,429 + +0.4
1,221,429 + -0.0
1,221,440 + +0.1
1,221,440 + +0.0
1,221,517 - -0.3
1,221,549 - +0.4
1,221,551 + -1.0
1,221,552 + +1.7
1,221,553 + +1.0
1,221,553 - +0.3
1,221,635 + b1171 b1171 0.32 -1.2
1,221,815 - b1171 b1171 0.87 -0.2
1,221,815 - b1171 b1171 0.87 -0.6
1,221,892 - +0.4
1,221,934 - ymgG b1172 0.19 -0.0
1,221,940 + ymgG b1172 0.21 +0.4
1,222,035 + ymgG b1172 0.49 +0.9
1,222,052 + ymgG b1172 0.54 +1.0
1,222,060 - ymgG b1172 0.56 +0.1
1,222,060 - ymgG b1172 0.56 +0.3
1,222,135 - ymgG b1172 0.78 +0.1
1,222,137 + ymgG b1172 0.78 +1.1
1,222,141 + ymgG b1172 0.79 +0.6
1,222,152 + ymgG b1172 0.83 +0.4
1,222,160 - ymgG b1172 0.85 -0.4
1,222,160 - ymgG b1172 0.85 +0.1
1,222,162 + ymgG b1172 0.86 +2.0
1,222,162 + ymgG b1172 0.86 +1.5
1,222,162 + ymgG b1172 0.86 +0.4
1,222,162 + ymgG b1172 0.86 +0.5
1,222,162 + ymgG b1172 0.86 -0.6
1,222,167 + ymgG b1172 0.87 -1.0
1,222,170 - ymgG b1172 0.88 -0.2
1,222,170 - ymgG b1172 0.88 +0.2
1,222,180 - +0.4
1,222,219 + -0.0
1,222,243 - ymgI b4593 0.17 -1.3
1,222,283 - ymgI b4593 0.40 -1.8
1,222,330 - ymgI b4593 0.67 +1.6
1,222,383 - +1.0
1,222,415 + -1.0
1,222,423 + -0.1
1,222,423 + +1.8
1,222,436 + +0.1
1,222,512 + ymgJ b4594 0.13 +1.0
1,222,568 + ymgJ b4594 0.44 -0.4
1,222,571 - ymgJ b4594 0.45 +1.1
1,222,571 - ymgJ b4594 0.45 +0.6
1,222,583 - ymgJ b4594 0.52 -0.0
1,222,630 - ymgJ b4594 0.77 +0.4
1,222,630 - ymgJ b4594 0.77 +0.0
1,222,659 - +0.1
1,222,703 - +0.2
1,222,703 - -0.2
1,222,703 - +0.2
1,222,722 - +0.8
1,222,753 + +0.0
1,222,760 + +0.3
1,222,760 + +0.5
1,222,763 + -0.2
1,222,768 - -0.0
1,222,783 + +0.7
1,222,846 + ycgI b4521 0.17 +0.2
1,222,857 - ycgI b4521 0.20 +0.5
1,222,981 + ycgI b4521 0.56 -0.3
1,223,000 + ycgI b4521 0.62 -0.4
1,223,000 + ycgI b4521 0.62 -0.0

Or see this region's nucleotide sequence