Strain Fitness in Escherichia coli BW25113 around b1053

Experiment: LB + carbenicillin Time4hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmdoH and yceK are separated by 172 nucleotidesyceK and msyB are separated by 0 nucleotidesmsyB and mdtG are separated by 82 nucleotidesmdtG and lpxL are separated by 171 nucleotides b1049: mdoH - glucosyltransferase MdoH (NCBI), at 1,110,086 to 1,112,629 mdoH b1050: yceK - predicted lipoprotein (NCBI), at 1,112,802 to 1,113,029 yceK b1051: msyB - acidic protein suppresses mutants lacking function of protein export (VIMSS), at 1,113,030 to 1,113,404 msyB b1053: mdtG - predicted drug efflux system (NCBI), at 1,113,487 to 1,114,713 mdtG b1054: lpxL - lipid A biosynthesis lauroyl acyltransferase (NCBI), at 1,114,885 to 1,115,805 lpxL Position (kb) 1113 1114 1115Strain fitness (log2 ratio) -2 -1 0 1at 1112.561 kb on - strandat 1112.670 kb on - strandat 1112.866 kb on + strand, within yceKat 1113.040 kb on + strandat 1113.040 kb on + strandat 1113.311 kb on - strand, within msyBat 1113.311 kb on - strand, within msyBat 1113.450 kb on - strandat 1113.524 kb on - strandat 1113.533 kb on + strandat 1113.533 kb on + strandat 1113.665 kb on - strandat 1113.703 kb on - strand, within mdtGat 1113.816 kb on + strand, within mdtGat 1113.893 kb on - strand, within mdtGat 1113.915 kb on + strand, within mdtGat 1113.915 kb on + strand, within mdtGat 1114.083 kb on - strand, within mdtGat 1114.083 kb on - strand, within mdtGat 1114.104 kb on + strand, within mdtGat 1114.136 kb on + strand, within mdtGat 1114.136 kb on + strand, within mdtGat 1114.274 kb on + strand, within mdtGat 1114.411 kb on + strand, within mdtGat 1114.411 kb on + strand, within mdtGat 1114.437 kb on + strand, within mdtGat 1114.508 kb on - strand, within mdtGat 1114.600 kb on - strandat 1114.600 kb on - strandat 1114.703 kb on - strandat 1114.833 kb on + strandat 1114.846 kb on - strandat 1114.889 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time4hr
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1,112,561 - +0.4
1,112,670 - -1.0
1,112,866 + yceK b1050 0.28 +1.3
1,113,040 + +0.5
1,113,040 + -2.0
1,113,311 - msyB b1051 0.75 +0.6
1,113,311 - msyB b1051 0.75 -1.1
1,113,450 - +0.1
1,113,524 - +0.2
1,113,533 + +0.2
1,113,533 + +0.3
1,113,665 - -0.7
1,113,703 - mdtG b1053 0.18 -0.0
1,113,816 + mdtG b1053 0.27 +0.3
1,113,893 - mdtG b1053 0.33 +0.1
1,113,915 + mdtG b1053 0.35 -0.3
1,113,915 + mdtG b1053 0.35 +0.2
1,114,083 - mdtG b1053 0.49 -0.2
1,114,083 - mdtG b1053 0.49 -0.5
1,114,104 + mdtG b1053 0.50 +0.2
1,114,136 + mdtG b1053 0.53 +0.1
1,114,136 + mdtG b1053 0.53 +0.7
1,114,274 + mdtG b1053 0.64 +0.2
1,114,411 + mdtG b1053 0.75 +0.7
1,114,411 + mdtG b1053 0.75 +0.8
1,114,437 + mdtG b1053 0.77 +0.8
1,114,508 - mdtG b1053 0.83 +1.0
1,114,600 - +0.1
1,114,600 - +0.3
1,114,703 - +0.0
1,114,833 + +0.1
1,114,846 - -0.2
1,114,889 - -0.0

Or see this region's nucleotide sequence