Experiment: LB + carbenicillin Time4hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaeQ and yaeJ overlap by 4 nucleotides yaeJ and nlpE are separated by 13 nucleotides nlpE and yaeF are separated by 199 nucleotides
b0190: yaeQ - hypothetical protein (NCBI), at 214,291 to 214,836
yaeQ
b0191: yaeJ - hypothetical protein (NCBI), at 214,833 to 215,255
yaeJ
b0192: nlpE - lipoprotein involved with copper homeostasis and adhesion (NCBI), at 215,269 to 215,979
nlpE
b0193: yaeF - orf, hypothetical protein (VIMSS), at 216,179 to 217,003
yaeF
Position (kb) 215
216 Strain fitness (log2 ratio) -1
0
1 at 214.369 kb on - strand, within yaeQ at 214.391 kb on - strand, within yaeQ at 214.404 kb on - strand, within yaeQ at 214.419 kb on - strand, within yaeQ at 214.468 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.542 kb on + strand, within yaeQ at 214.572 kb on + strand, within yaeQ at 214.574 kb on + strand, within yaeQ at 214.600 kb on - strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.764 kb on - strand, within yaeQ at 214.773 kb on - strand, within yaeQ at 214.808 kb on + strand at 214.825 kb on - strand at 214.836 kb on - strand at 214.872 kb on + strand at 214.874 kb on + strand at 214.919 kb on + strand, within yaeJ at 215.045 kb on + strand, within yaeJ at 215.045 kb on + strand, within yaeJ at 215.091 kb on + strand, within yaeJ at 215.123 kb on + strand, within yaeJ at 215.123 kb on + strand, within yaeJ at 215.299 kb on + strand at 215.372 kb on + strand, within nlpE at 215.378 kb on + strand, within nlpE at 215.410 kb on + strand, within nlpE at 215.476 kb on - strand, within nlpE at 215.498 kb on - strand, within nlpE at 215.498 kb on - strand, within nlpE at 215.558 kb on + strand, within nlpE at 215.582 kb on - strand, within nlpE at 215.621 kb on + strand, within nlpE at 215.635 kb on - strand, within nlpE at 215.635 kb on - strand, within nlpE at 215.694 kb on + strand, within nlpE at 215.756 kb on + strand, within nlpE at 215.816 kb on + strand, within nlpE at 215.816 kb on + strand, within nlpE at 215.824 kb on - strand, within nlpE at 215.864 kb on - strand, within nlpE at 215.864 kb on - strand, within nlpE at 215.895 kb on - strand, within nlpE at 215.895 kb on - strand, within nlpE at 215.897 kb on - strand, within nlpE at 215.915 kb on - strand at 215.915 kb on - strand at 215.931 kb on - strand at 215.931 kb on - strand at 215.933 kb on - strand at 215.933 kb on - strand at 215.958 kb on + strand at 215.968 kb on - strand at 216.101 kb on - strand at 216.101 kb on - strand at 216.101 kb on - strand at 216.151 kb on + strand at 216.159 kb on - strand at 216.159 kb on - strand at 216.160 kb on - strand at 216.160 kb on - strand at 216.235 kb on + strand at 216.235 kb on + strand at 216.282 kb on + strand, within yaeF at 216.285 kb on + strand, within yaeF at 216.285 kb on + strand, within yaeF at 216.326 kb on - strand, within yaeF at 216.332 kb on - strand, within yaeF at 216.332 kb on - strand, within yaeF at 216.334 kb on + strand, within yaeF at 216.334 kb on + strand, within yaeF at 216.342 kb on - strand, within yaeF at 216.342 kb on - strand, within yaeF at 216.417 kb on + strand, within yaeF at 216.417 kb on - strand, within yaeF at 216.417 kb on - strand, within yaeF at 216.444 kb on + strand, within yaeF at 216.505 kb on + strand, within yaeF at 216.635 kb on + strand, within yaeF at 216.733 kb on + strand, within yaeF at 216.749 kb on - strand, within yaeF at 216.774 kb on - strand, within yaeF at 216.786 kb on - strand, within yaeF at 216.804 kb on + strand, within yaeF at 216.868 kb on - strand, within yaeF at 216.896 kb on + strand, within yaeF at 216.896 kb on + strand, within yaeF
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time4hr remove 214,369 - yaeQ b0190 0.14 -0.3 214,391 - yaeQ b0190 0.18 +0.4 214,404 - yaeQ b0190 0.21 -0.2 214,419 - yaeQ b0190 0.23 -0.4 214,468 + yaeQ b0190 0.32 -0.1 214,487 + yaeQ b0190 0.36 +0.1 214,487 + yaeQ b0190 0.36 +0.2 214,542 + yaeQ b0190 0.46 -0.1 214,572 + yaeQ b0190 0.51 +0.1 214,574 + yaeQ b0190 0.52 -0.2 214,600 - yaeQ b0190 0.57 +0.2 214,606 + yaeQ b0190 0.58 +0.3 214,606 + yaeQ b0190 0.58 +0.2 214,764 - yaeQ b0190 0.87 -0.0 214,773 - yaeQ b0190 0.88 -0.0 214,808 + +0.5 214,825 - +0.3 214,836 - +0.4 214,872 + +0.2 214,874 + -0.1 214,919 + yaeJ b0191 0.20 +0.3 215,045 + yaeJ b0191 0.50 +0.1 215,045 + yaeJ b0191 0.50 +0.2 215,091 + yaeJ b0191 0.61 +0.4 215,123 + yaeJ b0191 0.69 -0.0 215,123 + yaeJ b0191 0.69 +0.1 215,299 + -0.0 215,372 + nlpE b0192 0.14 -0.1 215,378 + nlpE b0192 0.15 +0.3 215,410 + nlpE b0192 0.20 +0.1 215,476 - nlpE b0192 0.29 +0.2 215,498 - nlpE b0192 0.32 +0.7 215,498 - nlpE b0192 0.32 +0.1 215,558 + nlpE b0192 0.41 +0.3 215,582 - nlpE b0192 0.44 +0.0 215,621 + nlpE b0192 0.50 +0.0 215,635 - nlpE b0192 0.51 +1.4 215,635 - nlpE b0192 0.51 +0.9 215,694 + nlpE b0192 0.60 +0.0 215,756 + nlpE b0192 0.68 +1.1 215,816 + nlpE b0192 0.77 +0.4 215,816 + nlpE b0192 0.77 -0.1 215,824 - nlpE b0192 0.78 +0.0 215,864 - nlpE b0192 0.84 +0.4 215,864 - nlpE b0192 0.84 -0.4 215,895 - nlpE b0192 0.88 +0.9 215,895 - nlpE b0192 0.88 +0.1 215,897 - nlpE b0192 0.88 -0.2 215,915 - +0.1 215,915 - +0.2 215,931 - -0.1 215,931 - +0.4 215,933 - -0.2 215,933 - +0.3 215,958 + -0.2 215,968 - +0.5 216,101 - +0.2 216,101 - -0.3 216,101 - +0.1 216,151 + -0.0 216,159 - -0.0 216,159 - +0.0 216,160 - -0.2 216,160 - +0.0 216,235 + +0.3 216,235 + +0.1 216,282 + yaeF b0193 0.12 +0.1 216,285 + yaeF b0193 0.13 +0.1 216,285 + yaeF b0193 0.13 +0.3 216,326 - yaeF b0193 0.18 +0.6 216,332 - yaeF b0193 0.19 +0.1 216,332 - yaeF b0193 0.19 -0.5 216,334 + yaeF b0193 0.19 -0.1 216,334 + yaeF b0193 0.19 +0.2 216,342 - yaeF b0193 0.20 +0.2 216,342 - yaeF b0193 0.20 +0.2 216,417 + yaeF b0193 0.29 +0.0 216,417 - yaeF b0193 0.29 +0.3 216,417 - yaeF b0193 0.29 +0.4 216,444 + yaeF b0193 0.32 +0.2 216,505 + yaeF b0193 0.40 +1.0 216,635 + yaeF b0193 0.55 +0.3 216,733 + yaeF b0193 0.67 -0.1 216,749 - yaeF b0193 0.69 -0.2 216,774 - yaeF b0193 0.72 +0.0 216,786 - yaeF b0193 0.74 +0.4 216,804 + yaeF b0193 0.76 -0.1 216,868 - yaeF b0193 0.84 +0.6 216,896 + yaeF b0193 0.87 +0.2 216,896 + yaeF b0193 0.87 +0.3
Or see this region's nucleotide sequence