Strain Fitness in Escherichia coli BW25113 around b3100

Experiment: LB + ciprofloxacin Time3hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyqjC and yqjD are separated by 37 nucleotidesyqjD and yqjE are separated by 2 nucleotidesyqjE and yqjK overlap by 11 nucleotidesyqjK and yqjF are separated by 185 nucleotidesyqjF and yqjG are separated by 69 nucleotides b3097: yqjC - orf, hypothetical protein (VIMSS), at 3,246,991 to 3,247,359 yqjC b3098: yqjD - hypothetical protein (NCBI), at 3,247,397 to 3,247,702 yqjD b3099: yqjE - conserved inner membrane protein (NCBI), at 3,247,705 to 3,248,109 yqjE b3100: yqjK - hypothetical protein (NCBI), at 3,248,099 to 3,248,398 yqjK b3101: yqjF - orf, hypothetical protein (VIMSS), at 3,248,584 to 3,248,976 yqjF b3102: yqjG - predicted S-transferase (NCBI), at 3,249,046 to 3,250,032 yqjG Position (kb) 3248 3249Strain fitness (log2 ratio) -1 0 1at 3247.140 kb on + strand, within yqjCat 3247.140 kb on + strand, within yqjCat 3247.170 kb on + strand, within yqjCat 3247.177 kb on - strand, within yqjCat 3247.257 kb on - strand, within yqjCat 3247.257 kb on - strand, within yqjCat 3247.377 kb on - strandat 3247.382 kb on + strandat 3247.558 kb on + strand, within yqjDat 3247.616 kb on + strand, within yqjDat 3247.616 kb on + strand, within yqjDat 3247.649 kb on + strand, within yqjDat 3247.784 kb on + strand, within yqjEat 3247.801 kb on - strand, within yqjEat 3247.801 kb on - strand, within yqjEat 3247.995 kb on + strand, within yqjEat 3248.030 kb on + strand, within yqjEat 3248.030 kb on + strand, within yqjEat 3248.030 kb on + strand, within yqjEat 3248.041 kb on - strand, within yqjEat 3248.076 kb on + strandat 3248.140 kb on - strand, within yqjKat 3248.140 kb on - strand, within yqjKat 3248.162 kb on + strand, within yqjKat 3248.281 kb on + strand, within yqjKat 3248.281 kb on + strand, within yqjKat 3248.309 kb on - strand, within yqjKat 3248.309 kb on - strand, within yqjKat 3248.323 kb on - strand, within yqjKat 3248.326 kb on + strand, within yqjKat 3248.384 kb on - strandat 3248.676 kb on + strand, within yqjFat 3248.790 kb on + strand, within yqjFat 3248.797 kb on + strand, within yqjFat 3248.940 kb on + strandat 3248.948 kb on - strandat 3249.159 kb on - strand, within yqjGat 3249.159 kb on - strand, within yqjGat 3249.259 kb on - strand, within yqjGat 3249.267 kb on + strand, within yqjGat 3249.309 kb on - strand, within yqjGat 3249.309 kb on - strand, within yqjGat 3249.335 kb on + strand, within yqjGat 3249.347 kb on - strand, within yqjGat 3249.347 kb on - strand, within yqjG

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time3hr
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3,247,140 + yqjC b3097 0.40 +0.5
3,247,140 + yqjC b3097 0.40 +0.3
3,247,170 + yqjC b3097 0.49 -0.2
3,247,177 - yqjC b3097 0.50 -0.1
3,247,257 - yqjC b3097 0.72 -1.6
3,247,257 - yqjC b3097 0.72 -1.2
3,247,377 - +0.2
3,247,382 + -0.4
3,247,558 + yqjD b3098 0.53 -0.1
3,247,616 + yqjD b3098 0.72 -0.2
3,247,616 + yqjD b3098 0.72 -0.5
3,247,649 + yqjD b3098 0.82 -0.2
3,247,784 + yqjE b3099 0.20 +0.1
3,247,801 - yqjE b3099 0.24 -0.1
3,247,801 - yqjE b3099 0.24 -0.0
3,247,995 + yqjE b3099 0.72 -0.1
3,248,030 + yqjE b3099 0.80 -0.5
3,248,030 + yqjE b3099 0.80 +0.2
3,248,030 + yqjE b3099 0.80 +0.3
3,248,041 - yqjE b3099 0.83 +1.0
3,248,076 + -0.1
3,248,140 - yqjK b3100 0.14 +0.6
3,248,140 - yqjK b3100 0.14 +1.1
3,248,162 + yqjK b3100 0.21 +0.2
3,248,281 + yqjK b3100 0.61 +0.3
3,248,281 + yqjK b3100 0.61 +0.4
3,248,309 - yqjK b3100 0.70 +0.5
3,248,309 - yqjK b3100 0.70 +0.3
3,248,323 - yqjK b3100 0.75 -0.3
3,248,326 + yqjK b3100 0.76 -0.3
3,248,384 - -0.8
3,248,676 + yqjF b3101 0.23 -0.5
3,248,790 + yqjF b3101 0.52 -0.2
3,248,797 + yqjF b3101 0.54 +0.2
3,248,940 + +0.1
3,248,948 - -0.2
3,249,159 - yqjG b3102 0.11 -0.1
3,249,159 - yqjG b3102 0.11 +0.5
3,249,259 - yqjG b3102 0.22 -1.1
3,249,267 + yqjG b3102 0.22 +0.3
3,249,309 - yqjG b3102 0.27 +0.2
3,249,309 - yqjG b3102 0.27 +1.0
3,249,335 + yqjG b3102 0.29 +0.3
3,249,347 - yqjG b3102 0.30 -0.0
3,249,347 - yqjG b3102 0.30 +0.2

Or see this region's nucleotide sequence