Experiment: LB + ciprofloxacin Time3hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b2989 and hybG are separated by 122 nucleotides hybG and hybF are separated by 12 nucleotides hybF and hybE overlap by 8 nucleotides hybE and hybD overlap by 8 nucleotides hybD and hybC overlap by 1 nucleotides
b2989: b2989 - orf, hypothetical protein (VIMSS), at 3,136,749 to 3,137,615
b2989
b2990: hybG - hydrogenase 2 accessory protein (NCBI), at 3,137,738 to 3,137,986
hybG
b2991: hybF - protein involved with the maturation of hydrogenases 1 and 2 (NCBI), at 3,137,999 to 3,138,340
hybF
b2992: hybE - hydrogenase 2-specific chaperone (NCBI), at 3,138,333 to 3,138,821
hybE
b2993: hybD - predicted maturation element for hydrogenase 2 (NCBI), at 3,138,814 to 3,139,308
hybD
b2994: hybC - hydrogenase 2, large subunit (NCBI), at 3,139,308 to 3,141,011
hybC
Position (kb)
3138
3139 Strain fitness (log2 ratio)
-1
0
1 at 3137.339 kb on - strand, within b2989 at 3137.360 kb on + strand at 3137.372 kb on + strand, within b2989 at 3137.372 kb on + strand, within b2989 at 3137.406 kb on + strand, within b2989 at 3137.414 kb on - strand, within b2989 at 3137.463 kb on + strand, within b2989 at 3137.525 kb on - strand, within b2989 at 3137.528 kb on + strand, within b2989 at 3137.615 kb on - strand at 3137.615 kb on - strand at 3137.691 kb on - strand at 3137.700 kb on + strand at 3137.714 kb on + strand at 3137.717 kb on - strand at 3137.839 kb on - strand, within hybG at 3137.839 kb on - strand, within hybG at 3137.907 kb on + strand, within hybG at 3137.986 kb on + strand at 3137.986 kb on + strand at 3138.086 kb on + strand, within hybF at 3138.146 kb on - strand, within hybF at 3138.152 kb on + strand, within hybF at 3138.166 kb on + strand, within hybF at 3138.233 kb on - strand, within hybF at 3138.274 kb on - strand, within hybF at 3138.274 kb on - strand, within hybF at 3138.363 kb on + strand at 3138.474 kb on + strand, within hybE at 3138.474 kb on + strand, within hybE at 3138.640 kb on + strand, within hybE at 3138.640 kb on + strand, within hybE at 3138.649 kb on + strand, within hybE at 3138.658 kb on - strand, within hybE at 3138.707 kb on + strand, within hybE at 3138.715 kb on - strand, within hybE at 3138.839 kb on - strand at 3138.839 kb on - strand at 3138.928 kb on + strand, within hybD at 3139.075 kb on - strand, within hybD at 3139.075 kb on - strand, within hybD at 3139.157 kb on + strand, within hybD at 3139.227 kb on + strand, within hybD at 3139.242 kb on - strand, within hybD at 3139.245 kb on + strand, within hybD at 3139.288 kb on + strand at 3139.308 kb on + strand at 3139.371 kb on - strand at 3139.371 kb on - strand at 3139.379 kb on + strand at 3139.379 kb on + strand at 3139.379 kb on + strand at 3139.396 kb on + strand at 3139.443 kb on - strand at 3139.538 kb on - strand, within hybC at 3139.613 kb on - strand, within hybC at 3139.676 kb on + strand, within hybC at 3139.683 kb on + strand, within hybC at 3139.701 kb on + strand, within hybC at 3139.716 kb on + strand, within hybC at 3139.763 kb on + strand, within hybC
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time3hr remove 3,137,339 - b2989 b2989 0.68 -0.0 3,137,360 + +0.1 3,137,372 + b2989 b2989 0.72 +0.2 3,137,372 + b2989 b2989 0.72 -0.2 3,137,406 + b2989 b2989 0.76 +0.3 3,137,414 - b2989 b2989 0.77 +0.2 3,137,463 + b2989 b2989 0.82 +0.2 3,137,525 - b2989 b2989 0.90 +0.1 3,137,528 + b2989 b2989 0.90 -1.3 3,137,615 - +0.0 3,137,615 - +0.1 3,137,691 - -0.2 3,137,700 + +0.4 3,137,714 + +0.5 3,137,717 - +0.1 3,137,839 - hybG b2990 0.41 +0.0 3,137,839 - hybG b2990 0.41 -0.3 3,137,907 + hybG b2990 0.68 -0.0 3,137,986 + +0.2 3,137,986 + +0.1 3,138,086 + hybF b2991 0.25 +0.1 3,138,146 - hybF b2991 0.43 -0.3 3,138,152 + hybF b2991 0.45 -0.4 3,138,166 + hybF b2991 0.49 +0.1 3,138,233 - hybF b2991 0.68 -0.1 3,138,274 - hybF b2991 0.80 +0.0 3,138,274 - hybF b2991 0.80 +0.1 3,138,363 + -0.2 3,138,474 + hybE b2992 0.29 +0.1 3,138,474 + hybE b2992 0.29 +0.1 3,138,640 + hybE b2992 0.63 +1.0 3,138,640 + hybE b2992 0.63 +0.9 3,138,649 + hybE b2992 0.65 +0.2 3,138,658 - hybE b2992 0.66 +0.0 3,138,707 + hybE b2992 0.76 +0.3 3,138,715 - hybE b2992 0.78 -0.0 3,138,839 - -0.1 3,138,839 - +0.0 3,138,928 + hybD b2993 0.23 +0.2 3,139,075 - hybD b2993 0.53 -0.4 3,139,075 - hybD b2993 0.53 -0.3 3,139,157 + hybD b2993 0.69 -0.0 3,139,227 + hybD b2993 0.83 +0.2 3,139,242 - hybD b2993 0.86 +0.9 3,139,245 + hybD b2993 0.87 -0.3 3,139,288 + +0.3 3,139,308 + -0.9 3,139,371 - +0.1 3,139,371 - -0.2 3,139,379 + +0.3 3,139,379 + +0.2 3,139,379 + -0.1 3,139,396 + +0.2 3,139,443 - +0.5 3,139,538 - hybC b2994 0.13 +0.1 3,139,613 - hybC b2994 0.18 +0.1 3,139,676 + hybC b2994 0.22 +0.2 3,139,683 + hybC b2994 0.22 +0.1 3,139,701 + hybC b2994 0.23 +0.2 3,139,716 + hybC b2994 0.24 -1.0 3,139,763 + hybC b2994 0.27 +0.0
Or see this region's nucleotide sequence