Strain Fitness in Escherichia coli BW25113 around b2204

Experiment: LB + ciprofloxacin Time3hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnapC and napB are separated by 9 nucleotidesnapB and napH overlap by 4 nucleotidesnapH and napG overlap by 14 nucleotidesnapG and napA are separated by 6 nucleotides b2202: napC - nitrate reductase, cytochrome c-type, periplasmic (NCBI), at 2,295,679 to 2,296,281 napC b2203: napB - cytochrome c-type protein (VIMSS), at 2,296,291 to 2,296,740 napB b2204: napH - quinol dehydrogenase membrane component (NCBI), at 2,296,737 to 2,297,600 napH b2205: napG - quinol dehydrogenase periplasmic component (NCBI), at 2,297,587 to 2,298,282 napG b2206: napA - nitrate reductase, periplasmic, large subunit (NCBI), at 2,298,289 to 2,300,775 napA Position (kb) 2296 2297 2298Strain fitness (log2 ratio) -1 0 1at 2295.760 kb on + strand, within napCat 2295.760 kb on + strand, within napCat 2295.769 kb on + strand, within napCat 2295.806 kb on + strand, within napCat 2295.880 kb on + strand, within napCat 2295.921 kb on - strand, within napCat 2296.069 kb on - strand, within napCat 2296.154 kb on - strand, within napCat 2296.254 kb on + strandat 2296.254 kb on + strandat 2296.348 kb on + strand, within napBat 2296.348 kb on + strand, within napBat 2296.356 kb on + strand, within napBat 2296.356 kb on + strand, within napBat 2296.368 kb on + strand, within napBat 2296.431 kb on + strand, within napBat 2296.491 kb on + strand, within napBat 2296.491 kb on + strand, within napBat 2296.491 kb on - strand, within napBat 2296.545 kb on + strand, within napBat 2296.578 kb on + strand, within napBat 2296.578 kb on + strand, within napBat 2296.675 kb on + strand, within napBat 2296.695 kb on - strand, within napBat 2296.773 kb on + strandat 2296.773 kb on + strandat 2296.779 kb on - strandat 2296.880 kb on - strand, within napHat 2296.953 kb on + strand, within napHat 2297.095 kb on - strand, within napHat 2297.341 kb on + strand, within napHat 2297.444 kb on - strand, within napHat 2297.464 kb on + strand, within napHat 2297.476 kb on - strand, within napHat 2297.476 kb on - strand, within napHat 2297.517 kb on + strandat 2297.535 kb on - strandat 2297.535 kb on - strandat 2297.585 kb on - strandat 2297.585 kb on - strandat 2297.615 kb on - strandat 2297.676 kb on - strand, within napGat 2297.791 kb on + strand, within napGat 2297.798 kb on + strand, within napGat 2297.949 kb on + strand, within napGat 2297.953 kb on - strand, within napGat 2298.068 kb on + strand, within napGat 2298.073 kb on + strand, within napGat 2298.161 kb on - strand, within napGat 2298.190 kb on + strand, within napGat 2298.194 kb on + strand, within napGat 2298.340 kb on - strandat 2298.340 kb on - strandat 2298.469 kb on + strandat 2298.562 kb on + strand, within napAat 2298.565 kb on + strand, within napAat 2298.570 kb on - strand, within napAat 2298.572 kb on + strand, within napAat 2298.578 kb on - strand, within napAat 2298.582 kb on + strand, within napAat 2298.582 kb on + strand, within napA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time3hr
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2,295,760 + napC b2202 0.13 +0.6
2,295,760 + napC b2202 0.13 +0.4
2,295,769 + napC b2202 0.15 +0.3
2,295,806 + napC b2202 0.21 -0.6
2,295,880 + napC b2202 0.33 +0.5
2,295,921 - napC b2202 0.40 +0.4
2,296,069 - napC b2202 0.65 +0.2
2,296,154 - napC b2202 0.79 -0.2
2,296,254 + +0.4
2,296,254 + +0.3
2,296,348 + napB b2203 0.13 +0.2
2,296,348 + napB b2203 0.13 +0.2
2,296,356 + napB b2203 0.14 -0.1
2,296,356 + napB b2203 0.14 -0.1
2,296,368 + napB b2203 0.17 +0.1
2,296,431 + napB b2203 0.31 -0.2
2,296,491 + napB b2203 0.44 +0.3
2,296,491 + napB b2203 0.44 +0.3
2,296,491 - napB b2203 0.44 -0.6
2,296,545 + napB b2203 0.56 -0.3
2,296,578 + napB b2203 0.64 +0.6
2,296,578 + napB b2203 0.64 +0.1
2,296,675 + napB b2203 0.85 +0.0
2,296,695 - napB b2203 0.90 -0.2
2,296,773 + +0.5
2,296,773 + +0.6
2,296,779 - -0.4
2,296,880 - napH b2204 0.17 +0.5
2,296,953 + napH b2204 0.25 +1.2
2,297,095 - napH b2204 0.41 +0.1
2,297,341 + napH b2204 0.70 +0.4
2,297,444 - napH b2204 0.82 -0.1
2,297,464 + napH b2204 0.84 +0.1
2,297,476 - napH b2204 0.86 +0.1
2,297,476 - napH b2204 0.86 -0.1
2,297,517 + -0.9
2,297,535 - +0.2
2,297,535 - +0.9
2,297,585 - +0.2
2,297,585 - -0.1
2,297,615 - +0.4
2,297,676 - napG b2205 0.13 -0.2
2,297,791 + napG b2205 0.29 +0.1
2,297,798 + napG b2205 0.30 -0.1
2,297,949 + napG b2205 0.52 +0.3
2,297,953 - napG b2205 0.53 +0.2
2,298,068 + napG b2205 0.69 -0.7
2,298,073 + napG b2205 0.70 -0.1
2,298,161 - napG b2205 0.82 -0.1
2,298,190 + napG b2205 0.87 -0.3
2,298,194 + napG b2205 0.87 -0.4
2,298,340 - -0.1
2,298,340 - +0.0
2,298,469 + -0.1
2,298,562 + napA b2206 0.11 +0.6
2,298,565 + napA b2206 0.11 +0.4
2,298,570 - napA b2206 0.11 -0.1
2,298,572 + napA b2206 0.11 +0.1
2,298,578 - napA b2206 0.12 -0.5
2,298,582 + napA b2206 0.12 +0.8
2,298,582 + napA b2206 0.12 +0.1

Or see this region's nucleotide sequence