Strain Fitness in Escherichia coli BW25113 around b1415

Experiment: LB + ciprofloxacin Time3hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydcF and aldA are separated by 196 nucleotidesaldA and gapC are separated by 41 nucleotides b1414: ydcF - hypothetical protein (NCBI), at 1,485,259 to 1,486,059 ydcF b1415: aldA - Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data), at 1,486,256 to 1,487,695 aldA b4493: gapC - no description, at 1,487,737 to 1,488,737 gapC Position (kb) 1486 1487 1488Strain fitness (log2 ratio) -1 0 1at 1485.446 kb on + strand, within ydcFat 1485.502 kb on + strand, within ydcFat 1485.553 kb on - strand, within ydcFat 1485.833 kb on + strand, within ydcFat 1485.869 kb on - strand, within ydcFat 1485.881 kb on + strand, within ydcFat 1485.881 kb on + strand, within ydcFat 1485.887 kb on - strand, within ydcFat 1485.899 kb on - strand, within ydcFat 1485.899 kb on - strand, within ydcFat 1486.182 kb on - strandat 1486.274 kb on - strandat 1486.276 kb on - strandat 1486.276 kb on - strandat 1486.392 kb on + strandat 1486.392 kb on + strandat 1486.401 kb on - strand, within aldAat 1486.401 kb on - strand, within aldAat 1486.721 kb on - strand, within aldAat 1486.932 kb on + strand, within aldAat 1486.932 kb on + strand, within aldAat 1487.097 kb on + strand, within aldAat 1487.294 kb on - strand, within aldAat 1487.351 kb on - strand, within aldAat 1487.490 kb on + strand, within aldAat 1487.801 kb on + strandat 1487.801 kb on + strandat 1487.921 kb on + strand, within gapCat 1488.063 kb on - strand, within gapCat 1488.092 kb on - strand, within gapCat 1488.654 kb on - strandat 1488.684 kb on + strandat 1488.684 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time3hr
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1,485,446 + ydcF b1414 0.23 +0.3
1,485,502 + ydcF b1414 0.30 +0.2
1,485,553 - ydcF b1414 0.37 -0.1
1,485,833 + ydcF b1414 0.72 +0.3
1,485,869 - ydcF b1414 0.76 -0.1
1,485,881 + ydcF b1414 0.78 +0.5
1,485,881 + ydcF b1414 0.78 +0.5
1,485,887 - ydcF b1414 0.78 +0.4
1,485,899 - ydcF b1414 0.80 -0.2
1,485,899 - ydcF b1414 0.80 -0.2
1,486,182 - +0.3
1,486,274 - +0.2
1,486,276 - +0.0
1,486,276 - -0.2
1,486,392 + +0.7
1,486,392 + +0.4
1,486,401 - aldA b1415 0.10 +0.3
1,486,401 - aldA b1415 0.10 +0.6
1,486,721 - aldA b1415 0.32 -0.5
1,486,932 + aldA b1415 0.47 +0.0
1,486,932 + aldA b1415 0.47 +0.1
1,487,097 + aldA b1415 0.58 -0.1
1,487,294 - aldA b1415 0.72 +0.1
1,487,351 - aldA b1415 0.76 +1.1
1,487,490 + aldA b1415 0.86 +0.5
1,487,801 + +0.9
1,487,801 + -0.4
1,487,921 + gapC b4493 0.18 -0.9
1,488,063 - gapC b4493 0.33 +0.3
1,488,092 - gapC b4493 0.35 +0.0
1,488,654 - +0.2
1,488,684 + +0.4
1,488,684 + +0.1

Or see this region's nucleotide sequence