Strain Fitness in Escherichia coli BW25113 around b3037

Experiment: LB + carbenicillin Time1-2hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttolC and ygiB are separated by 147 nucleotidesygiB and ygiC are separated by 5 nucleotides b3035: tolC - outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS), at 3,176,137 to 3,177,618 tolC b3037: ygiB - conserved outer membrane protein (RefSeq), at 3,177,766 to 3,178,437 ygiB b3038: ygiC - predicted enzyme (NCBI), at 3,178,443 to 3,179,603 ygiC Position (kb) 3177 3178 3179Strain fitness (log2 ratio) -3 -2 -1 0 1at 3176.844 kb on - strand, within tolCat 3176.844 kb on - strand, within tolCat 3176.869 kb on - strand, within tolCat 3176.869 kb on - strand, within tolCat 3176.887 kb on + strand, within tolCat 3176.887 kb on + strand, within tolCat 3176.887 kb on + strand, within tolCat 3176.932 kb on + strand, within tolCat 3176.934 kb on + strand, within tolCat 3176.942 kb on - strand, within tolCat 3176.979 kb on - strand, within tolCat 3176.979 kb on - strand, within tolCat 3176.981 kb on + strand, within tolCat 3176.981 kb on + strand, within tolCat 3176.988 kb on - strand, within tolCat 3177.093 kb on + strand, within tolCat 3177.105 kb on + strand, within tolCat 3177.113 kb on - strand, within tolCat 3177.120 kb on + strand, within tolCat 3177.339 kb on + strand, within tolCat 3177.339 kb on + strand, within tolCat 3177.437 kb on + strand, within tolCat 3177.488 kb on + strandat 3177.516 kb on - strandat 3177.517 kb on + strandat 3177.517 kb on + strandat 3177.717 kb on + strandat 3177.737 kb on + strandat 3177.747 kb on - strandat 3177.964 kb on + strand, within ygiBat 3177.964 kb on + strand, within ygiBat 3177.964 kb on + strand, within ygiBat 3177.964 kb on - strand, within ygiBat 3178.163 kb on + strand, within ygiBat 3178.238 kb on + strand, within ygiBat 3178.238 kb on + strand, within ygiBat 3178.276 kb on + strand, within ygiBat 3178.276 kb on + strand, within ygiBat 3178.294 kb on - strand, within ygiBat 3178.298 kb on - strand, within ygiBat 3178.298 kb on - strand, within ygiBat 3178.390 kb on + strandat 3178.390 kb on + strandat 3178.398 kb on - strandat 3178.423 kb on - strandat 3178.454 kb on + strandat 3178.540 kb on - strandat 3178.542 kb on + strandat 3178.545 kb on - strandat 3178.546 kb on - strandat 3178.546 kb on - strandat 3178.653 kb on - strand, within ygiCat 3178.754 kb on - strand, within ygiCat 3178.765 kb on - strand, within ygiCat 3178.900 kb on - strand, within ygiCat 3178.900 kb on - strand, within ygiCat 3178.900 kb on - strand, within ygiCat 3178.927 kb on + strand, within ygiCat 3178.927 kb on + strand, within ygiCat 3178.946 kb on + strand, within ygiCat 3178.989 kb on + strand, within ygiCat 3178.997 kb on - strand, within ygiCat 3179.016 kb on + strand, within ygiCat 3179.016 kb on + strand, within ygiCat 3179.016 kb on + strand, within ygiCat 3179.016 kb on + strand, within ygiCat 3179.024 kb on - strand, within ygiCat 3179.026 kb on + strand, within ygiCat 3179.057 kb on + strand, within ygiCat 3179.078 kb on - strand, within ygiCat 3179.078 kb on - strand, within ygiCat 3179.105 kb on + strand, within ygiCat 3179.179 kb on - strand, within ygiCat 3179.179 kb on - strand, within ygiCat 3179.196 kb on + strand, within ygiCat 3179.196 kb on + strand, within ygiCat 3179.253 kb on + strand, within ygiC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time1-2hr
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3,176,844 - tolC b3035 0.48 +0.7
3,176,844 - tolC b3035 0.48 +0.7
3,176,869 - tolC b3035 0.49 -0.9
3,176,869 - tolC b3035 0.49 +0.7
3,176,887 + tolC b3035 0.51 +0.7
3,176,887 + tolC b3035 0.51 -1.9
3,176,887 + tolC b3035 0.51 -1.6
3,176,932 + tolC b3035 0.54 -0.3
3,176,934 + tolC b3035 0.54 -1.9
3,176,942 - tolC b3035 0.54 -1.3
3,176,979 - tolC b3035 0.57 -0.3
3,176,979 - tolC b3035 0.57 +0.7
3,176,981 + tolC b3035 0.57 +0.7
3,176,981 + tolC b3035 0.57 +0.7
3,176,988 - tolC b3035 0.57 -2.3
3,177,093 + tolC b3035 0.65 +0.7
3,177,105 + tolC b3035 0.65 -2.4
3,177,113 - tolC b3035 0.66 +0.7
3,177,120 + tolC b3035 0.66 -1.9
3,177,339 + tolC b3035 0.81 -0.3
3,177,339 + tolC b3035 0.81 +0.7
3,177,437 + tolC b3035 0.88 -3.5
3,177,488 + -0.4
3,177,516 - -2.2
3,177,517 + -0.1
3,177,517 + -0.0
3,177,717 + +0.1
3,177,737 + -0.0
3,177,747 - -0.1
3,177,964 + ygiB b3037 0.29 +1.6
3,177,964 + ygiB b3037 0.29 +0.4
3,177,964 + ygiB b3037 0.29 +0.4
3,177,964 - ygiB b3037 0.29 +0.2
3,178,163 + ygiB b3037 0.59 +0.0
3,178,238 + ygiB b3037 0.70 +0.3
3,178,238 + ygiB b3037 0.70 +0.4
3,178,276 + ygiB b3037 0.76 +0.4
3,178,276 + ygiB b3037 0.76 +0.5
3,178,294 - ygiB b3037 0.79 -0.3
3,178,298 - ygiB b3037 0.79 -0.8
3,178,298 - ygiB b3037 0.79 -0.4
3,178,390 + -0.2
3,178,390 + -1.3
3,178,398 - -0.2
3,178,423 - -0.1
3,178,454 + -0.3
3,178,540 - -0.7
3,178,542 + +0.2
3,178,545 - +0.1
3,178,546 - +0.6
3,178,546 - -0.3
3,178,653 - ygiC b3038 0.18 -0.8
3,178,754 - ygiC b3038 0.27 +0.2
3,178,765 - ygiC b3038 0.28 -0.0
3,178,900 - ygiC b3038 0.39 +0.1
3,178,900 - ygiC b3038 0.39 +0.2
3,178,900 - ygiC b3038 0.39 -0.0
3,178,927 + ygiC b3038 0.42 -0.1
3,178,927 + ygiC b3038 0.42 -1.0
3,178,946 + ygiC b3038 0.43 +0.2
3,178,989 + ygiC b3038 0.47 -0.1
3,178,997 - ygiC b3038 0.48 -0.3
3,179,016 + ygiC b3038 0.49 +0.3
3,179,016 + ygiC b3038 0.49 -0.3
3,179,016 + ygiC b3038 0.49 +0.0
3,179,016 + ygiC b3038 0.49 -0.1
3,179,024 - ygiC b3038 0.50 -0.2
3,179,026 + ygiC b3038 0.50 -0.1
3,179,057 + ygiC b3038 0.53 -0.3
3,179,078 - ygiC b3038 0.55 -0.0
3,179,078 - ygiC b3038 0.55 -0.2
3,179,105 + ygiC b3038 0.57 -0.0
3,179,179 - ygiC b3038 0.63 -0.4
3,179,179 - ygiC b3038 0.63 -0.4
3,179,196 + ygiC b3038 0.65 -0.0
3,179,196 + ygiC b3038 0.65 -0.1
3,179,253 + ygiC b3038 0.70 -0.3

Or see this region's nucleotide sequence