Experiment: LB + carbenicillin Time1-2hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfdQ and b2361 are separated by 127 nucleotides b2361 and yfdS overlap by 10 nucleotides yfdS and yfdT overlap by 1 nucleotides yfdT and ypdJ are separated by 2 nucleotides ypdJ and torI are separated by 78 nucleotides torI and pawZ are separated by 73 nucleotides pawZ and dsdC are separated by 95 nucleotides
b2360: yfdQ - CPS-53 (KpLE1) prophage; predicted protein (NCBI), at 2,472,054 to 2,472,878
yfdQ
b2361: b2361 - orf, hypothetical protein (VIMSS), at 2,473,006 to 2,473,542
b2361
b2362: yfdS - CPS-53 (KpLE1) prophage; predicted protein (NCBI), at 2,473,533 to 2,473,895
yfdS
b2363: yfdT - CPS-53 (KpLE1) prophage; predicted protein (NCBI), at 2,473,895 to 2,474,200
yfdT
b4545: ypdJ - no description, at 2,474,203 to 2,474,253
ypdJ
b4501: torI - response regulator inhibitor for tor operon (NCBI), at 2,474,332 to 2,474,532
torI
b4643: pawZ - tRNA-OTHER (RefSeq), at 2,474,606 to 2,474,620
pawZ
b2364: dsdC - DNA-binding transcriptional dual regulator (NCBI), at 2,474,716 to 2,475,651
dsdC
Position (kb)
2473
2474 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2472.577 kb on - strand, within yfdQ at 2472.577 kb on - strand, within yfdQ at 2472.578 kb on - strand, within yfdQ at 2472.591 kb on - strand, within yfdQ at 2472.618 kb on + strand, within yfdQ at 2472.618 kb on + strand, within yfdQ at 2472.626 kb on - strand, within yfdQ at 2472.626 kb on - strand, within yfdQ at 2472.630 kb on + strand, within yfdQ at 2472.679 kb on - strand, within yfdQ at 2472.684 kb on + strand, within yfdQ at 2472.684 kb on + strand, within yfdQ at 2472.686 kb on + strand, within yfdQ at 2472.686 kb on + strand, within yfdQ at 2472.704 kb on - strand, within yfdQ at 2472.731 kb on + strand, within yfdQ at 2472.745 kb on + strand, within yfdQ at 2472.745 kb on + strand, within yfdQ at 2472.745 kb on + strand, within yfdQ at 2472.745 kb on + strand, within yfdQ at 2472.745 kb on + strand, within yfdQ at 2472.745 kb on + strand, within yfdQ at 2472.745 kb on + strand, within yfdQ at 2472.745 kb on + strand, within yfdQ at 2472.746 kb on + strand, within yfdQ at 2472.750 kb on + strand, within yfdQ at 2472.759 kb on - strand, within yfdQ at 2472.763 kb on + strand, within yfdQ at 2472.763 kb on + strand, within yfdQ at 2472.768 kb on + strand, within yfdQ at 2472.773 kb on + strand, within yfdQ at 2472.783 kb on + strand, within yfdQ at 2472.783 kb on + strand, within yfdQ at 2472.896 kb on + strand at 2472.896 kb on + strand at 2472.906 kb on - strand at 2472.943 kb on + strand at 2472.943 kb on + strand at 2472.946 kb on + strand at 2472.946 kb on + strand at 2472.951 kb on + strand at 2472.951 kb on + strand at 2472.952 kb on - strand at 2472.954 kb on - strand at 2472.954 kb on - strand at 2472.958 kb on + strand at 2472.958 kb on + strand at 2472.958 kb on + strand at 2472.962 kb on + strand at 2472.974 kb on + strand at 2472.974 kb on + strand at 2472.977 kb on - strand at 2472.979 kb on + strand at 2472.979 kb on + strand at 2472.979 kb on + strand at 2472.979 kb on + strand at 2472.979 kb on + strand at 2472.979 kb on + strand at 2472.984 kb on + strand at 2472.984 kb on + strand at 2472.985 kb on + strand at 2472.986 kb on + strand at 2472.987 kb on + strand at 2472.987 kb on + strand at 2472.987 kb on + strand at 2472.989 kb on + strand at 2472.992 kb on - strand at 2472.992 kb on - strand at 2472.992 kb on - strand at 2472.995 kb on - strand at 2473.009 kb on - strand at 2473.061 kb on - strand, within b2361 at 2473.061 kb on - strand, within b2361 at 2473.124 kb on + strand, within b2361 at 2473.134 kb on + strand, within b2361 at 2473.134 kb on + strand, within b2361 at 2473.137 kb on + strand, within b2361 at 2473.142 kb on + strand, within b2361 at 2473.142 kb on - strand, within b2361 at 2473.142 kb on - strand, within b2361 at 2473.142 kb on - strand, within b2361 at 2473.142 kb on - strand, within b2361 at 2473.142 kb on - strand, within b2361 at 2473.145 kb on - strand, within b2361 at 2473.145 kb on - strand, within b2361 at 2473.148 kb on + strand, within b2361 at 2473.150 kb on - strand, within b2361 at 2473.150 kb on - strand, within b2361 at 2473.152 kb on - strand, within b2361 at 2473.152 kb on - strand, within b2361 at 2473.155 kb on - strand, within b2361 at 2473.155 kb on - strand, within b2361 at 2473.156 kb on - strand, within b2361 at 2473.156 kb on - strand, within b2361 at 2473.171 kb on - strand, within b2361 at 2473.201 kb on + strand, within b2361 at 2473.222 kb on - strand, within b2361 at 2473.222 kb on - strand, within b2361 at 2473.239 kb on - strand, within b2361 at 2473.240 kb on - strand, within b2361 at 2473.241 kb on + strand, within b2361 at 2473.242 kb on + strand, within b2361 at 2473.249 kb on - strand, within b2361 at 2473.257 kb on - strand, within b2361 at 2473.257 kb on - strand, within b2361 at 2473.260 kb on - strand, within b2361 at 2473.264 kb on - strand, within b2361 at 2473.300 kb on + strand, within b2361 at 2473.300 kb on + strand, within b2361 at 2473.315 kb on + strand, within b2361 at 2473.333 kb on + strand, within b2361 at 2473.333 kb on + strand, within b2361 at 2473.341 kb on - strand, within b2361 at 2473.351 kb on - strand, within b2361 at 2473.410 kb on - strand, within b2361 at 2473.445 kb on - strand, within b2361 at 2473.479 kb on - strand, within b2361 at 2473.607 kb on + strand, within yfdS at 2473.627 kb on - strand, within yfdS at 2473.627 kb on - strand, within yfdS at 2473.629 kb on + strand, within yfdS at 2473.649 kb on + strand, within yfdS at 2473.724 kb on + strand, within yfdS at 2473.740 kb on + strand, within yfdS at 2473.763 kb on + strand, within yfdS at 2473.770 kb on + strand, within yfdS at 2473.788 kb on - strand, within yfdS at 2473.788 kb on - strand, within yfdS at 2473.866 kb on + strand at 2473.888 kb on + strand at 2473.888 kb on + strand at 2473.950 kb on + strand, within yfdT at 2473.950 kb on + strand, within yfdT at 2473.950 kb on + strand, within yfdT at 2473.950 kb on + strand, within yfdT at 2473.970 kb on + strand, within yfdT at 2473.982 kb on + strand, within yfdT at 2474.110 kb on - strand, within yfdT at 2474.119 kb on + strand, within yfdT at 2474.127 kb on - strand, within yfdT at 2474.213 kb on - strand, within ypdJ at 2474.221 kb on - strand, within ypdJ at 2474.227 kb on - strand, within ypdJ at 2474.316 kb on + strand at 2474.316 kb on + strand at 2474.320 kb on - strand at 2474.329 kb on + strand at 2474.343 kb on + strand at 2474.344 kb on + strand at 2474.352 kb on - strand, within torI at 2474.390 kb on - strand, within torI at 2474.390 kb on - strand, within torI at 2474.453 kb on - strand, within torI at 2474.480 kb on + strand, within torI at 2474.634 kb on + strand at 2474.668 kb on + strand at 2474.689 kb on - strand at 2474.691 kb on + strand at 2474.734 kb on - strand at 2474.734 kb on - strand at 2474.747 kb on - strand at 2474.809 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time1-2hr remove 2,472,577 - yfdQ b2360 0.63 +1.0 2,472,577 - yfdQ b2360 0.63 +1.0 2,472,578 - yfdQ b2360 0.64 +1.0 2,472,591 - yfdQ b2360 0.65 +1.0 2,472,618 + yfdQ b2360 0.68 +0.4 2,472,618 + yfdQ b2360 0.68 -0.3 2,472,626 - yfdQ b2360 0.69 +0.6 2,472,626 - yfdQ b2360 0.69 -0.3 2,472,630 + yfdQ b2360 0.70 -1.4 2,472,679 - yfdQ b2360 0.76 +1.0 2,472,684 + yfdQ b2360 0.76 -0.4 2,472,684 + yfdQ b2360 0.76 -0.0 2,472,686 + yfdQ b2360 0.77 +0.1 2,472,686 + yfdQ b2360 0.77 -0.2 2,472,704 - yfdQ b2360 0.79 +1.0 2,472,731 + yfdQ b2360 0.82 -0.1 2,472,745 + yfdQ b2360 0.84 +0.5 2,472,745 + yfdQ b2360 0.84 -1.0 2,472,745 + yfdQ b2360 0.84 -0.2 2,472,745 + yfdQ b2360 0.84 +0.4 2,472,745 + yfdQ b2360 0.84 +0.4 2,472,745 + yfdQ b2360 0.84 +0.7 2,472,745 + yfdQ b2360 0.84 -0.1 2,472,745 + yfdQ b2360 0.84 -0.0 2,472,746 + yfdQ b2360 0.84 +1.0 2,472,750 + yfdQ b2360 0.84 -0.7 2,472,759 - yfdQ b2360 0.85 +1.0 2,472,763 + yfdQ b2360 0.86 -0.1 2,472,763 + yfdQ b2360 0.86 -0.4 2,472,768 + yfdQ b2360 0.87 -0.2 2,472,773 + yfdQ b2360 0.87 +0.0 2,472,783 + yfdQ b2360 0.88 -0.2 2,472,783 + yfdQ b2360 0.88 -1.2 2,472,896 + -0.8 2,472,896 + -0.0 2,472,906 - +1.0 2,472,943 + -1.6 2,472,943 + +0.5 2,472,946 + +1.0 2,472,946 + +1.0 2,472,951 + -0.0 2,472,951 + +1.6 2,472,952 - +0.6 2,472,954 - -0.2 2,472,954 - +0.4 2,472,958 + -0.0 2,472,958 + -1.0 2,472,958 + -0.6 2,472,962 + -0.0 2,472,974 + +1.4 2,472,974 + +0.8 2,472,977 - +0.2 2,472,979 + +0.2 2,472,979 + -0.7 2,472,979 + -0.2 2,472,979 + -0.4 2,472,979 + -0.3 2,472,979 + +0.1 2,472,984 + -0.0 2,472,984 + -0.4 2,472,985 + -0.0 2,472,986 + -0.5 2,472,987 + +2.8 2,472,987 + -1.0 2,472,987 + -1.2 2,472,989 + +0.2 2,472,992 - +0.6 2,472,992 - +0.1 2,472,992 - +0.5 2,472,995 - -0.0 2,473,009 - -3.0 2,473,061 - b2361 b2361 0.10 +0.7 2,473,061 - b2361 b2361 0.10 -0.0 2,473,124 + b2361 b2361 0.22 -0.3 2,473,134 + b2361 b2361 0.24 +0.2 2,473,134 + b2361 b2361 0.24 -0.9 2,473,137 + b2361 b2361 0.24 -0.9 2,473,142 + b2361 b2361 0.25 +1.0 2,473,142 - b2361 b2361 0.25 -0.4 2,473,142 - b2361 b2361 0.25 -0.3 2,473,142 - b2361 b2361 0.25 -0.1 2,473,142 - b2361 b2361 0.25 +0.4 2,473,142 - b2361 b2361 0.25 -0.1 2,473,145 - b2361 b2361 0.26 +0.2 2,473,145 - b2361 b2361 0.26 -0.9 2,473,148 + b2361 b2361 0.26 +0.3 2,473,150 - b2361 b2361 0.27 +0.4 2,473,150 - b2361 b2361 0.27 +0.3 2,473,152 - b2361 b2361 0.27 -0.8 2,473,152 - b2361 b2361 0.27 +0.2 2,473,155 - b2361 b2361 0.28 +0.5 2,473,155 - b2361 b2361 0.28 -0.4 2,473,156 - b2361 b2361 0.28 -0.1 2,473,156 - b2361 b2361 0.28 +0.4 2,473,171 - b2361 b2361 0.31 -0.2 2,473,201 + b2361 b2361 0.36 +0.4 2,473,222 - b2361 b2361 0.40 -0.4 2,473,222 - b2361 b2361 0.40 +0.2 2,473,239 - b2361 b2361 0.43 +0.0 2,473,240 - b2361 b2361 0.44 +0.3 2,473,241 + b2361 b2361 0.44 -0.3 2,473,242 + b2361 b2361 0.44 +0.8 2,473,249 - b2361 b2361 0.45 +0.3 2,473,257 - b2361 b2361 0.47 -0.1 2,473,257 - b2361 b2361 0.47 -0.1 2,473,260 - b2361 b2361 0.47 +1.4 2,473,264 - b2361 b2361 0.48 +0.0 2,473,300 + b2361 b2361 0.55 -0.8 2,473,300 + b2361 b2361 0.55 -0.1 2,473,315 + b2361 b2361 0.58 +1.6 2,473,333 + b2361 b2361 0.61 -0.1 2,473,333 + b2361 b2361 0.61 -0.2 2,473,341 - b2361 b2361 0.62 -0.5 2,473,351 - b2361 b2361 0.64 -1.0 2,473,410 - b2361 b2361 0.75 +0.4 2,473,445 - b2361 b2361 0.82 -0.0 2,473,479 - b2361 b2361 0.88 +0.0 2,473,607 + yfdS b2362 0.20 +4.1 2,473,627 - yfdS b2362 0.26 +1.0 2,473,627 - yfdS b2362 0.26 +1.0 2,473,629 + yfdS b2362 0.26 -0.1 2,473,649 + yfdS b2362 0.32 +0.3 2,473,724 + yfdS b2362 0.53 +0.5 2,473,740 + yfdS b2362 0.57 -0.3 2,473,763 + yfdS b2362 0.63 +0.1 2,473,770 + yfdS b2362 0.65 -0.0 2,473,788 - yfdS b2362 0.70 -1.0 2,473,788 - yfdS b2362 0.70 +1.2 2,473,866 + -1.0 2,473,888 + +0.4 2,473,888 + +0.4 2,473,950 + yfdT b2363 0.18 -0.4 2,473,950 + yfdT b2363 0.18 +0.0 2,473,950 + yfdT b2363 0.18 +0.1 2,473,950 + yfdT b2363 0.18 +1.7 2,473,970 + yfdT b2363 0.25 -0.0 2,473,982 + yfdT b2363 0.28 -1.0 2,474,110 - yfdT b2363 0.70 -0.8 2,474,119 + yfdT b2363 0.73 +1.0 2,474,127 - yfdT b2363 0.76 -0.4 2,474,213 - ypdJ b4545 0.20 -0.2 2,474,221 - ypdJ b4545 0.35 -1.4 2,474,227 - ypdJ b4545 0.47 +0.2 2,474,316 + -0.4 2,474,316 + -0.7 2,474,320 - -0.0 2,474,329 + -0.2 2,474,343 + -0.2 2,474,344 + -0.4 2,474,352 - torI b4501 0.10 -0.3 2,474,390 - torI b4501 0.29 -1.0 2,474,390 - torI b4501 0.29 -0.3 2,474,453 - torI b4501 0.60 -0.4 2,474,480 + torI b4501 0.74 +0.0 2,474,634 + +0.1 2,474,668 + -1.0 2,474,689 - +0.3 2,474,691 + +0.0 2,474,734 - +0.0 2,474,734 - +0.4 2,474,747 - +0.8 2,474,809 - +0.1
Or see this region's nucleotide sequence