Strain Fitness in Escherichia coli BW25113 around b1976

Experiment: LB + carbenicillin Time1-2hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyodB and serU are separated by 569 nucleotidesserU and b1976 are separated by 93 nucleotidesb1976 and asnT are separated by 100 nucleotidesasnT and yeeJ are separated by 313 nucleotides b1974: yodB - putative cytochrome (VIMSS), at 2,040,392 to 2,040,922 yodB b1975: serU - tRNA-Ser (NCBI), at 2,041,492 to 2,041,581 serU b1976: b1976 - orf, hypothetical protein (VIMSS), at 2,041,675 to 2,042,472 b1976 b1977: asnT - tRNA-Asn (NCBI), at 2,042,573 to 2,042,648 asnT b1978: yeeJ - adhesin (RefSeq), at 2,042,962 to 2,050,038 yeeJ Position (kb) 2041 2042 2043Strain fitness (log2 ratio) -1 0 1 2at 2040.681 kb on + strand, within yodBat 2040.681 kb on + strand, within yodBat 2040.755 kb on + strand, within yodBat 2040.757 kb on + strand, within yodBat 2040.767 kb on - strand, within yodBat 2040.772 kb on - strand, within yodBat 2040.772 kb on - strand, within yodBat 2040.860 kb on - strand, within yodBat 2040.957 kb on + strandat 2040.957 kb on + strandat 2040.992 kb on + strandat 2041.038 kb on + strandat 2041.054 kb on - strandat 2041.236 kb on + strandat 2041.236 kb on + strandat 2041.359 kb on - strandat 2041.359 kb on - strandat 2041.395 kb on + strandat 2041.515 kb on - strand, within serUat 2041.871 kb on + strand, within b1976at 2041.999 kb on - strand, within b1976at 2042.029 kb on + strand, within b1976at 2042.037 kb on - strand, within b1976at 2042.042 kb on + strand, within b1976at 2042.055 kb on - strand, within b1976at 2042.095 kb on + strand, within b1976at 2042.160 kb on - strand, within b1976at 2042.160 kb on - strand, within b1976at 2042.403 kb on + strandat 2042.418 kb on - strandat 2042.434 kb on + strandat 2042.434 kb on + strandat 2042.619 kb on + strand, within asnTat 2042.619 kb on + strand, within asnTat 2042.747 kb on + strandat 2042.824 kb on - strandat 2042.917 kb on + strandat 2042.917 kb on + strandat 2042.923 kb on - strandat 2042.923 kb on - strandat 2042.923 kb on - strandat 2042.925 kb on - strandat 2042.963 kb on + strandat 2042.963 kb on + strandat 2042.972 kb on - strandat 2043.094 kb on + strandat 2043.094 kb on + strandat 2043.118 kb on + strandat 2043.176 kb on + strandat 2043.184 kb on - strandat 2043.319 kb on - strandat 2043.354 kb on + strandat 2043.432 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time1-2hr
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2,040,681 + yodB b1974 0.54 -0.4
2,040,681 + yodB b1974 0.54 +2.4
2,040,755 + yodB b1974 0.68 -0.2
2,040,757 + yodB b1974 0.69 +0.5
2,040,767 - yodB b1974 0.71 -0.3
2,040,772 - yodB b1974 0.72 +0.2
2,040,772 - yodB b1974 0.72 -0.2
2,040,860 - yodB b1974 0.88 -0.6
2,040,957 + +0.3
2,040,957 + -0.4
2,040,992 + +2.4
2,041,038 + -0.5
2,041,054 - +0.3
2,041,236 + +0.4
2,041,236 + +0.1
2,041,359 - -0.3
2,041,359 - +0.9
2,041,395 + -0.2
2,041,515 - serU b1975 0.26 +0.2
2,041,871 + b1976 b1976 0.25 +0.3
2,041,999 - b1976 b1976 0.41 +0.2
2,042,029 + b1976 b1976 0.44 +0.6
2,042,037 - b1976 b1976 0.45 +0.1
2,042,042 + b1976 b1976 0.46 +0.8
2,042,055 - b1976 b1976 0.48 +0.5
2,042,095 + b1976 b1976 0.53 +0.4
2,042,160 - b1976 b1976 0.61 +0.0
2,042,160 - b1976 b1976 0.61 +2.7
2,042,403 + +0.1
2,042,418 - +0.2
2,042,434 + +0.1
2,042,434 + +0.5
2,042,619 + asnT b1977 0.61 -0.4
2,042,619 + asnT b1977 0.61 +0.1
2,042,747 + -0.6
2,042,824 - -0.8
2,042,917 + -0.1
2,042,917 + +0.3
2,042,923 - +0.3
2,042,923 - +0.5
2,042,923 - +0.7
2,042,925 - -0.7
2,042,963 + -0.1
2,042,963 + -1.0
2,042,972 - +0.1
2,043,094 + +0.8
2,043,094 + +0.6
2,043,118 + -0.2
2,043,176 + -0.5
2,043,184 - +0.3
2,043,319 - +0.7
2,043,354 + -0.6
2,043,432 - +0.5

Or see this region's nucleotide sequence